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Analysis of the siderophore genes in Nocardia

Research Project

Project/Area Number 19710170
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied genomics
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

HOSHINO Yasutaka  National Institute of Infectious Diseases (40399457)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsシデロフォア / ノカルジア / ゲノム / 生合成遺伝子 / ノコバクチン / nocobactin / nocardia / 二次代謝産物 / 生合成
Research Abstract

Recently, we have determined the complete genome sequence of Nocardia farcinica IFM 10152 and found gene clusters (nbt gene clusters) thought to be responsible for the biosynthesis of siderophore in the genome. In this study, as expected, a siderophore was isolated from this strain and identified as nocobactin NA. The chemical structure is in good agreement with the gene organization of the nbt gene clusters. Furthermore, gene disruption and heterologous expression experiments demonstrated that nbt gene clusters were responsible for the biosynthesis of nocobactin NA.
To our knowledge, this is the first example of the biosynthetic genes and pathway of mycobactin type siderophore in the Nocardia. Furthermore, this work highlights the importance of genome analysis in combination with metabolite analysis.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (4 results)

  • [Journal Article] Nocardia terpenica sp. nov.,isolated from Japanese patients with nocardiosis2007

    • Author(s)
      Yasutaka Hoshino(1番目)、Jun Ishikawa、Yuzuru Mikami, その他7名
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology Vol.57

      Pages: 1456-1460

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nocardia terpenica sp.nov.,isolated from Japanese patients with nocardiosis2007

    • Author(s)
      Yasutaka Hoshino
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 57

      Pages: 1456-1460

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification of two gene clusters for nocobactin biosynthesis in Nocardia farcinica2008

    • Author(s)
      Yasutaka Hoshino
    • Organizer
      UK-Japan Workshop on Genomics of Antibiotic-producing Actinomycetes: Implications and Applications
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 病原性放線菌Nocardia farcinicaにおけるシデロフォアnocobactinの生合成遺伝子の同定2008

    • Author(s)
      星野泰隆
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of two gene clusters for nocobactin biosynthesis in Nocardia farcinica.2008

    • Author(s)
      Yasutaka Hoshino
    • Organizer
      UK-Japan Workshop on Genomics of Antibiotic-producing Actinomycetes: Implications and Applications
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] 病原性放線菌Nocardia farcinicaにおけるシデロフォアnocobactinの生合成遺伝子の同定2008

    • Author(s)
      星野 泰隆
    • Organizer
      第81回目本細菌学会総会
    • Place of Presentation
      京都
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification of the salicylate synthase gene of Nocardia farcinica and its role in the biosynthesis of nocobactin2007

    • Author(s)
      Yasutaka Hoshino
    • Organizer
      International Symposium on the Biology of Actinomycetes
    • Place of Presentation
      ニューキャッスル(英国)
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of a genetic analysis system for Nocardia species2007

    • Author(s)
      Jun Ishikawa
    • Organizer
      International Symposium on the Biology of Actinomycetes
    • Place of Presentation
      ニユーキヤツスル(英国)
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] 病原性放線菌Nocardia属64種が産生するシデロフォアの多様性の解析2007

    • Author(s)
      五ノ井透
    • Organizer
      第22回日本放線菌学会大会
    • Place of Presentation
      広島
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] Identification of the salicylate synthase gene of Nocardia farcinica and its role in the biosynthesis of nocobactin.2007

    • Author(s)
      yasutaka Hoshino
    • Organizer
      International Symposium on the Biology of Actinomycetes
    • Place of Presentation
      ニューキャッスル(英国)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://nocardia.nih.go.jp/

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://nocardia.nih.go.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://nocardia.nih.go.jp/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://nocardia.nih.go.jp/

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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