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Micro-culture device for environmental condition control and quantitative electrochemical analysis

Research Project

Project/Area Number 19750055
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionTohoku University

Principal Investigator

SHIKU Hitoshi  Tohoku University, 大学院・環境科学研究科, 准教授 (10361164)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsチップ分析 / 電気化学 / レポーター遺伝子 / 細胞培養
Research Abstract

我々はこれまでに、ベータ・ガラクトシダーゼ(βGAL)およびアルカリホスファターゼ(AP)をレポーター酵素とし、遺伝子発現のon/offにより制御されるシグナル伝達の電気化学的追跡が可能であることを示した。本課題では、単一細胞レベルでの酵素活性検出を目標とする。これを実現するために、分泌型酵素を利用しレポーター酵素を膜外に分泌させ、大幅な感度の向上を目指す(分泌型APの適用)。電極・ウェルデザインの最適化により酵素反応生成物が効率的に電極に到達するよう検討を加える。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (32 results)

All 2009 2008 2007 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (11 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Development of Electrochemical Reporter Assay Using HeLa Cells Transfected with Vector PLasmids Encoding Various Responsive Elements2009

    • Author(s)
      H. Shiku, M. Takeda, T. Murata, U. Akiba, F. Hamada, T. Matsue
    • Journal Title

      Anal. Chim. Acta 640

      Pages: 87-92

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochcmical Charactcrization of Enzymatic Activity of Ycast Cells Entrapped in a Poly(dimethylsiloxane)Microwell on the Basis of Limited Diffusion System2009

    • Author(s)
      H. Shiku, S. Goto, S. Jung, K. Nagamine, M. Koide, T. Itayama, T. Yasukawa, T. Matsue
    • Journal Title

      Analyst 134

      Pages: 182-187

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A microfluidic dual capillary probe to collect messenger RNA from adherent cells and spheroids2009

    • Author(s)
      H. Shiku, T. Yamakawa, Y. Nashimoto, Y. Takahashi, Y. Torisawa, T. Yasukawa, T. Ito-Sasaki, M. Yokoo, H. Abe, H. Kambara, T. Matsue
    • Journal Title

      Analytical Biochemistry 385

      Pages: 138-142

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Entrapment and Measurement of a Biologically Functionalized Microbead with a Microwell Electrode2009

    • Author(s)
      C. -Y. Chang, T. Murata, Y. Takahashi, H. Shiku, H. -C. Chang, T. Matsue
    • Journal Title

      Lab Chip 2009,9

      Pages: 1185-1192

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy2009

    • Author(s)
      . Takahashi, T. Miyamoto, H. Shiku, R. Asano, T. Yasukawa, I. Kumagai, T. Matsue
    • Journal Title

      Anal. Chem. 81(7)

      Pages: 2785-2790

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Characterization of Enzymatic Activity of Yeast Cells Entrapped in a Poly (dimethylsiloxane) Microwell on the Basis of Limited Diffusion System2009

    • Author(s)
      H. Shiku, et.al.
    • Journal Title

      Analyst 134

      Pages: 182-187

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A microfluidic dual capillary probe to collect messenger RNA from adherent cells and spheroidsAnalytical Biochemistry2009

    • Author(s)
      H. Shiku, et.al.
    • Journal Title

      Analytical Biochemistry 385

      Pages: 138-142

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical gene-function analysis for single cells with addressable electrode/microwell arrays2009

    • Author(s)
      Z. Lin, et.al.
    • Journal Title

      Angew. Chem. Mt. Ed. 48

      Pages: 2044-2046

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Entrapment and Measurement of a Biologically Functionalized Microbead with a Microwell Electrode2009

    • Author(s)
      C.-Y. Chang, et.al.
    • Journal Title

      Lab Chip 9(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Detection of Epidermal Growth Factor Receptors on a Si ngle Living Cell Surface by Scanning Electrochemical Microscopy2009

    • Author(s)
      Y. Takahashi, et.al.
    • Journal Title

      Anal. Chem 81

      Pages: 2785-2790

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Electrochemical Reporter Assay Using HeLa Cells Transfected with Vector Plasmids Encoding Various Responsive Elements2009

    • Author(s)
      H. Shiku, et.al.
    • Journal Title

      Anal. Chim. Acta 640

      Pages: 87-92

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrophoretic cell manipulation and elettrochemical gene-function analys is based on a yeast two-hybrid system in a microfluidic device2008

    • Author(s)
      T. Yasukawa, et.al.
    • Journal Title

      Anal. Chem 80

      Pages: 3722-3727

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An addressable microelectrode array for electrochemical detection2008

    • Author(s)
      Z. Lin, et.al.
    • Journal Title

      Anal. Chem 80

      Pages: 6830-6833

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryos2007

    • Author(s)
      C. -C. Wu, T. Saito, T. Yasukawa, H. Shiku, H. Abe, H. Hoshi, T. Matsue
    • Journal Title

      Sens. Actuat. B 125

      Pages: 680-687

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy(SECM)2007

    • Author(s)
      K. Nagamine, et. al.
    • Journal Title

      Biotechnol. Bioeng. 96

      Pages: 1008-1013

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A multicellular spheroid array chip to realize spheroid formation, culture and viability assay2007

    • Author(s)
      Y. Torisawa, et. al.
    • Journal Title

      Biomaterials 28

      Pages: 559-566

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryos2007

    • Author(s)
      Y. Nashimoto, et. al.
    • Journal Title

      Analyitical Chemistry 79

      Pages: 6823-6830

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Single-cell analysis based on scanning probe technique and electrochemical microdevice2009

    • Author(s)
      H. Shiku
    • Organizer
      日本化学会第89回年会アジア国際シンポジウム
    • Place of Presentation
      日本大学理工学部舟橋キャンパス(招待講演)
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 一細胞採取およびPCR解析に基づくデジタル解析2009

    • Author(s)
      珠玖 仁
    • Organizer
      第8回日本再生医療学会総会
    • Place of Presentation
      東京国際フォーラム, 東京都千代田区(招待講演)
    • Year and Date
      2009-03-05
    • Related Report
      2008 Final Research Report
  • [Presentation] 一細胞採取およびPCR解析に基づくデジタル解析2009

    • Author(s)
      珠玖仁
    • Organizer
      第8回日本再生医療学会総会
    • Place of Presentation
      東京国際フォーラム, 東京都(招待講演)
    • Year and Date
      2009-03-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Single-cell gene expression analysis based on scanning probe microscopy2008

    • Author(s)
      H. Shiku, Y. Takahashi, T. Murata, T. Matsue
    • Organizer
      IUMRS international conference in Asia 2008
    • Place of Presentation
      Nagoya Congress Center, Nagoya
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] SECM (scanning electrochemical microscopy) for single cell bioimaging2008

    • Author(s)
      H. Shiku
    • Organizer
      PRiME 2008, 214th Meeting of The Electrochemical Society
    • Place of Presentation
      Honolulu, Hawaii (USA)
    • Year and Date
      2008-10-16
    • Related Report
      2008 Final Research Report
  • [Presentation] Scanning Probe technique and electrochemical microdevice for single cell analysis2008

    • Author(s)
      H. Shiku
    • Organizer
      JAIST Nano Technology Symposium 2008
    • Place of Presentation
      Ishikawa High-Tech Conference Center, Ishikawa(招待講演)
    • Related Report
      2008 Final Research Report
  • [Presentation] Electrochemical Analysis of Yeast Cells Expressing Beta-Galactosidase within a Poly (dimethylsiloxane) Microwell Array2008

    • Author(s)
      H. Shiku, S. Goto, K. Nagamine, T. Yasukawa, T. Itayama, T. Matsue
    • Organizer
      5^<th> Workshop on Scanning electrochemical Microscopy
    • Place of Presentation
      Blue Mountain Lake, NY (USA)
    • Related Report
      2008 Final Research Report
  • [Presentation] マイクロ・コンタクトプリンティング法におけるタンパク質パターニング効率の電気化学的定量評価2008

    • Author(s)
      珠玖 仁,熊谷 絢子,羅 紅群,高橋 康史,安川 智之,山田 弘,末永 智一
    • Organizer
      電気化学会第75回大会
    • Place of Presentation
      山梨大学
    • Related Report
      2008 Final Research Report
  • [Presentation] 密閉系マイクロウェルアレイによる酵母の電気化学評価2008

    • Author(s)
      珠玖 仁,後藤 俊,チョン ソンボン,長峯 邦明,安川 智之,末永 智一
    • Organizer
      電気化学会第75回大会
    • Place of Presentation
      山梨大学
    • Related Report
      2008 Final Research Report
  • [Presentation] Oxygen Consumption of Mammalian Embryos and Oocytes Monitored by Scanning Electrochemical Microscopy2007

    • Author(s)
      H. Shiku, T. Yasukawa, T. Matsue, T. Ito-Sasaki, M. Yokoo, H. Abe
    • Organizer
      IEEE Sensors 2007
    • Place of Presentation
      Atlanta Georgia USA
    • Related Report
      2008 Final Research Report
  • [Presentation] 単一細胞由来mRNA回収プローブと電気化学マイクロシステムの開発(招待講演)2007

    • Author(s)
      珠玖 仁
    • Organizer
      生体機能関連化学部会若手の会サマースクール
    • Place of Presentation
      八王子セミナーハウス、東京
    • Related Report
      2007 Annual Research Report
  • [Book] Bioelectrochemstry : fundamentals, experimental techniques, and applications2008

    • Author(s)
      H. Shiku, K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue
    • Publisher
      Chapter 7, John Wiley & Sons Ltd., West Sussex, UK. (Eds. P. N. Bartlett)
    • Related Report
      2008 Final Research Report
  • [Book] John Wiley & Sons Ltd., West Sussex, UK.(Eds.P.N.Bartlett)2008

    • Author(s)
      H. Shiku, et.al.
    • Publisher
      Bioelectrochemistry : fundamentals, experimental techniques, and applications, Chapter 7
    • Related Report
      2008 Annual Research Report
  • [Book] 「生体分子の局所分析と電気化学イメージング」、(バイオ電気化学の実際-バイオセンサ・バイオ電池の実用展開-、監修 池田篤治 、第13章2007

    • Author(s)
      高橋康史, 他
    • Publisher
      シーエムシー出版
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://db.tohoku.ac.jp/whois/detail/acd9a15637a319e79930f9ff86a2b16c.html

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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