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Development of fluorescent sensors for intracellular transition metal ions and their applications to ratiometric imaging

Research Project

Project/Area Number 19750139
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionKyoto University

Principal Investigator

TAKI Masayasu  Kyoto University, 大学院・地球環境学堂, 助教 (70378850)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywords生物無機化学 / 化学センサー / 金属イオンセンサー / 蛍光 / イメージング / レシオ測定 / カドミウムイオン / 水銀イオン / 銀イオン / 蛍光プローブ / レシオ型 / 選択性
Research Abstract

生体内における金属イオンの動的挙動を解析するためのツール分子として種々のセンサー分子の開発を行った。その結果、銀、カドミウム、亜鉛、および水銀などの金属イオンに対して特異的な応答を示す分子の開発に成功した。中でもカドミウムイオンセンサーを用いることで、レシオ法による細胞内カドミウムの濃度変化を世界で初めてイメージングすることに成功した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (36 results)

All 2009 2008 2007 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (23 results) Remarks (2 results)

  • [Journal Article] Development of ratiometric fluorescent probe for zinc ion based on indole fluoronhore2009

    • Author(s)
      M. Taki, Y. Watanabe, Y. Yamamoto
    • Journal Title

      Tetrahedron Letters 50

      Pages: 1345-1347

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Rosamine-based Fluorescent Chemosensor for Selective Detection of Silver(I) in Aqueous Solution2008

    • Author(s)
      Shohei Iyoshi, Masayasu Taki, Yukio Yamamoto
    • Journal Title

      Inorganic Chemistry 47

      Pages: 3946-3948

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects on Heteroatom Substitution on the Structures, Physicochemical Properties, and Redox Behavior of Nickel(II) Complexes with Pyridine-Containing Macrocyclic Ligands2008

    • Author(s)
      Masayasu Taki, Yoshiaki Kawashima, Naoko Sakai, Tasuku Hirayama, Yukio Yamamoto
    • Journal Title

      Bulletin of the Chemical Society of Japan 81

      Pages: 590-597

    • NAID

      10021075440

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Colorimetric Response to Mercury -induced Abstraction of Triethylene Glycol Ligand from Gold Nanoparticle Surface2008

    • Author(s)
      Tasuku Hirayama, Masayasu Taki, Yukiyasu Kashiwagi, Masami Nakamoto, Atsushi Kunishita, Shinobu Itoh, Yukio Yamamoto
    • Journal Title

      Dalton Transactions

      Pages: 4705-4707

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Imaging of Intracellular Cadmium Using a Dual-Excitation Ratiometric Chemosensor2008

    • Author(s)
      Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto
    • Journal Title

      Journal of the American Chemical Society 130

      Pages: 12564-12565

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Rosamine-based Fluorescent Chemosensor for Selective Detection of Silver(I) in Aqueous Solution2008

    • Author(s)
      S. Iyoshi, M. Taki, Y. Yamamoto
    • Journal Title

      Inorganic Chemistry 47

      Pages: 3946-3948

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects on Heteroatom Substitution on the Structures, Physicochemical Properties, and Redox Behavior of Nickel(II) Complexes with Pyridine-Containing Macrocyclic Ligands2008

    • Author(s)
      M. Taki, Y. Kawashima, N. Sakai, T. Hiravama Y. Yamamoto
    • Journal Title

      Bulletin of the Chemical Society of Japan 81

      Pages: 590-597

    • NAID

      10021075440

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Colorimetric Response to Mercury -induced Abstraction of Triethylene Glycol Ligand from Gold Nanonarticle Surface2008

    • Author(s)
      T. Hirayama, M. Taki, Y. Kashiwagi, M. Nakamoto, A. Kunishita, S. Itoh, Y. Yamamoto
    • Journal Title

      Dalton Transactions

      Pages: 4705-4707

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Imaging of Intracellular Cadmium Using a Dual-Excitation Ratiometric Chemosensor2008

    • Author(s)
      M. Taki, M. Desaki, A. Ojida, S. Iyoshi, T. Hirayama, I. Hamachi, Y. Yamamoto
    • Journal Title

      Journal of the American Chemical Society 130

      Pages: 12564-12565

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model peptide of troponin C2007

    • Author(s)
      Tasuku Hirayama, Syohei Iyoshi, Masa Taki, Yuichiro Maeda, Yukio Yamamoto
    • Journal Title

      Organic & Biomolecular Chemistry 5

      Pages: 2040-2045

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model2007

    • Author(s)
      T. Hirayama, S. Iyoshi, M. Taki, Y. Maeda, Y. Yamamoto
    • Journal Title

      Organic & Biomolecular Chemistry 5

      Pages: 2040-2045

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ピリジン環含有環状ニッケル錯体と外部配位子との結合挙動2009

    • Author(s)
      旭史悦・多喜正泰・山本行男
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] チオエーテル系多座配位子による銅-価酸化還元挙動の制御および隣接蛍光画の発光挙動に与える影響2009

    • Author(s)
      伊吉祥平・多喜正泰・山本行男
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヌンチャク型ペプチドを利用した希土類亜鉛蛍光センサーの開発2009

    • Author(s)
      平山祐・多喜正泰・山本行男
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Ratiometric Fluorescence Imaging of Intracellular Cadmium Using a Coumarin-based Chemocensor2008

    • Author(s)
      S. lyoshi, M. Taki, A. Ojida, I. Hamachi, Y Yamamoto
    • Organizer
      The 4th Asian Biological Inorganic Chemistry Conference
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2008-11-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] チオエーテル含有多座配位子を用いた銀および銅(I)錯体の構造と発光特性2008

    • Author(s)
      伊吉祥平・多喜正泰・山本行男
    • Organizer
      第58回錯体化学討論会
    • Place of Presentation
      金沢大学
    • Year and Date
      2008-09-21
    • Related Report
      2008 Final Research Report
  • [Presentation] チオエーテル含有多座配位子を用いた銀および銅(1)錯体の構造と発光特性2008

    • Author(s)
      伊吉祥平・多喜正泰・山本行男
    • Organizer
      第58回錯体化学討論会
    • Place of Presentation
      金沢大学
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規ピリジン含有環状ニッケル錯体の構造と物性および外部配位子との挙動2008

    • Author(s)
      旭史悦・多喜正泰・山本行男
    • Organizer
      第58回錯体化学討論会
    • Place of Presentation
      金尺大学
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 外部配位子との配位挙動に及ぼす環状ニッケル錯体の置換基効果2008

    • Author(s)
      若松俊彦・多喜正泰・山本行男
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学
    • Year and Date
      2008-03-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] タンパク質の可逆的蛍光ラベル化を指向した二官能基性ローダミン分子の開発2008

    • Author(s)
      春木秀仁・平山祐・多喜正泰・山本行男
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] エチレングリコール担持金クラスターによる水銀イオンの特異的検出とその反応機構2008

    • Author(s)
      平山祐・多喜正泰・山本行男
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] チオエーテル配位子を有するロサミン型蛍光センサーの構造と機能2008

    • Author(s)
      伊吉祥平・多喜正泰・山本行男
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学
    • Year and Date
      2008-03-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Ratiometric Fluorescence Imaging of Intracellular Cadmium Using a Coumarin-based Chemosensor2008

    • Author(s)
      Shohei Iyoshi, Masayasu Taki, Akio Ojida, Itaru Hamachi, Yukio Yamamoto
    • Organizer
      The 4th Asian Biological Inorganic Chemistry Conference
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2008 Final Research Report
  • [Presentation] Membrane-permeable Fluorescent Chemo-sensor for Ratiometric Imaging of Cadmium in Living Cells2007

    • Author(s)
      Masaasu Taki and Yukio Yamamoto
    • Organizer
      Biomimetics Conference
    • Place of Presentation
      Doshisha, Kyoto, Japan
    • Year and Date
      2007-12-20
    • Related Report
      2008 Final Research Report
  • [Presentation] Membrane-permeable Fluorescent Chemosensor for Ratiometric Imaging of Cadmium in2007

    • Author(s)
      Masayasu Taki and Yukio Yamamto
    • Organizer
      Biomimetics Conference
    • Place of Presentation
      Doshisha University
    • Year and Date
      2007-12-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of New Lanthanide Complexes Binding to Peptide Tag2007

    • Author(s)
      〓suku Hirayama, Masayasu Taki, Yukio Yamamoto
    • Organizer
      Biomimetics Conference
    • Place of Presentation
      Doshisha University
    • Year and Date
      2007-12-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] アミノクマリンを骨格とした新規レシオ型カドミウム蛍光プローブの開発2007

    • Author(s)
      多喜正泰・出崎美佳・山本行男
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2007-09-28
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] 新しいペプチドタグ結合性希土類錯体の開発2007

    • Author(s)
      平山祐・多喜正泰・山本行男
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2007-09-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] エチレングリコール担持金クラスターを用いた水銀の特異的検出とその反応メカニズム2007

    • Author(s)
      平山祐・多喜正泰・山本行男
    • Organizer
      第57回錯体化学討論会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] チオエーテル配位子を有するロサミン型蛍光プローブの開発と金属イオンに対する蛍光挙動2007

    • Author(s)
      伊吉祥平・多喜正泰・山本行男
    • Organizer
      第57回錯体化学討論会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] タンパク質蛍光ラベル化を目指したペプチド結合性二ッケル錯体の合成と性質2007

    • Author(s)
      若松俊彦・平山祐・多喜正泰・山本行男
    • Organizer
      第57回錯体化学討論会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Highly Selective Ratiometric Fluorescent Probe with Picomolar sensitivity for Cadmium2007

    • Author(s)
      Masayasu Taki and Yukio Yamamoto
    • Organizer
      13th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      University of Vienna
    • Year and Date
      2007-07-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Mechanism of colorimetric sensing of Hg2+ with triethyleneglycol-supported gold nano-cluster2007

    • Author(s)
      Tasuku Hirayama, Masayasu Taki, Yukio Yamamoto
    • Organizer
      13th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      University of Vienna
    • Year and Date
      2007-07-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Highly Selective Ratiometric Fluorescent Probe with Picomolar sensitivity for Cadmium2007

    • Author(s)
      Masayasu Taki and Yukio Yamamoto
    • Organizer
      13th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.users.kudpe.kyoto-u.ac/jp/~t50544/

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.h.kyoto-u.acjp/staff/321_taki_m_O_j.html

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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