Design of pyrene-labeled oligonucleotide probes for SNps genotyping

• Project/Area Number: 19750146
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Nihon University
• Principal Investigator: SAITO Yoshlo Nihon University, 工学部, 講師 (40385985)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,320,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: ビスピレン / SNPs / エキシマー / DNA

Research Abstract

本研究では、ビスピレン構造を有するユニットを核酸塩某に導入し、その向かいに特定の塩基が来たときにのみエキシマーを形成するようなプローブの開発を行った。実際にビスピレンユニットを含むプローブの開発に成功し、ピレンエキシマー発光により標的DNAの有無を確認できた。

Research Products

• [Journal Article] C8-alkynyl-and alkylamino substituted 2'-deoxyguanosines : A universal linker for nucleic acids modification (2008)
  • Author(s): Yoshio Saito, Katsuhiko Matsumoto, lsao Saito
  • Journal Title: Tetrahedron 64 Pages: 3578-3588
  • Peer Reviewed
• [Journal Article] C8-alkynyl- and alkylamino substituted T-deoxyguanosines : A universal linker for nucleic acids modification (2008)
  • Author(s): Toshio Saito
  • Journal Title: Tetrahedron 64 Pages: 3578-3588
  • Peer Reviewed
• [Journal Article] Isao Saito, Design of a novel G-quenched molecular beacon : A simple and efficient strategy for DNA sequence analysis (2007)
  • Author(s): CD Yoshio Saito, Erika Mizuno, Sekhar S. Bag, Isamu Suzuka
  • Journal Title: Chem. Commun Pages: 4492-4494
  • Peer Reviewed
• [Journal Article] Design of dual-labeled oligonucleotide probes for SNPs genotyping (2007)
  • Author(s): Yoshio Saito, Subhendu Sekhar Bag, Satoshi Kodate, Isamu Suzuka, Isao Saito
  • Journal Title: Nucleic Acids Symp. Ser vol.51 Pages: 23-24
• [Journal Article] Design of a novel G-quenched molecular beacon: A simple and efficient strategy for DNA sequence analysis (2007)
  • Author(s): Yoshio Saito
  • Journal Title: Chem. Commun. Pages: 4492-4494
  • Peer Reviewed
• [Presentation] 診断のためのクエンチャー分子を用いないモレキュラービーコンの開発 (2008)
  • Author(s): 齋藤義雄、水野絵梨香、沼尻恭子、BAG Subhendu Sekhar、齋藤烈、遺伝子
  • Organizer: 第30回日本光医学・光生物学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-07-18
• [Presentation] 遺伝子診断のためのクエンチャー分子を用いないモレキュラービーコンの開発 (2008)
  • Author(s): 齋藤義雄
  • Organizer: 第30回日本光医学・光生物学会
  • Place of Presentation: 松江市
  • Year and Date: 2008-07-11
• [Presentation] FRETを用いた塩基識別型蛍光性核酸塩基の開発 (2007)
  • Author(s): 齋藤義雄
  • Organizer: 第29回日本光医学・光生物学会
  • Place of Presentation: 富山市
  • Year and Date: 2007-07-13
• [Presentation] FRETを用いた塩基識別型蛍光性核酸塩基の開発 (2007)
  • Author(s): 齋藤義雄、松本桂彦、水野絵梨香、小舘知史、鈴鹿敢、齋藤烈
  • Organizer: 第29回日本光医学・光生物学会
  • Place of Presentation: 冨山市
  • Year and Date: 2007-03-13
• [Remarks]
  • URL: http://kenkyu-web.cin.nihon-u.ac.jp/Profiles/JA/0006756/profile.html