Development of Single Molecule Analysis and Mathematical Model for Biomacromolecules Flowing Near Solid-liquid Interface

• Project/Area Number: 19760117
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Fluid engineering
• Research Institution: Osaka University
• Principal Investigator: SHINTAKU Hirofumi Osaka University, 基礎工学研究科, 助教 (80448050)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: バイオ流体工学 / ナノバイオ / 界面動電位 / 壁面効果 / 1分子計測 / 生体高分子微小流路 / 電場計測 / 電気二重層 / 生体高分子 / 微小流路 / 界面

Research Abstract

In this research, we developed a measurement method for electrokinetic phenomena and a numerical model for biomacromolecules to clarify the detailed dynamics of biomacromolecules flowing near solid-liquid interfaces. The dynamics including electro osmosis flow and electrophoresis in micro- and nano- channels were investigated using the developed methods. The result obtained here suggested that the electrokinetic phenomena could be used for the development of a novel single molecule analysis to investigate properties of biomacromolecules.

Research Products

• [Journal Article] Developments of Coarse Graining DNA Models for Single Nucleotide Resolution Analysis (2010)
  • Author(s): Kentaro DOI, Tomoaki HAGA, Hirofumi SHINTAKU, Satoyuki KAWANO
  • Journal Title: Phil. Trans. R. Soc. A Vol.368 Pages: 2615-2628
  • Peer Reviewed
• [Journal Article] Oxygenation to Bovine Blood in Artificial Heart and Lung Using Vibrating Flow Pump: Experiment and Numerical Analysis Based on Non-Newtonian Model (2010)
  • Author(s): Hirofumi SHINTAKU, Tsubasa YONEMURA, Kazuaki TSURU, Takashi ISOYAMA, Tomoyuki YAMBE, Satoyuki KAWANO
  • Journal Title: Journal of Fluid Science and Technology Vol.5,No.2 Pages: 292-304
  • Peer Reviewed
• [Journal Article] Measurements of Electric Field and Electrokinetic Phenomena Using Two Kinds of Tracer Particles with Different Mobilities (2009)
  • Author(s): Hirofumi SHINTAKU, Shuji AZUMA, Satoyuki KAWANO
  • Journal Title: Journal of Fluid Science and Technology Vol.4,No.3 Pages: 687-698
  • Peer Reviewed
• [Journal Article] 移動度の異なるトレーサ粒子を用いた電場と界面動電現象の計測 (2009)
  • Author(s): 新宅博文, 東修士, 川野聡恭
  • Journal Title: 日本機械学会論文集(B編) 第75巻,753号 Pages: 945-952
  • Peer Reviewed
• [Journal Article] Structural and Tensile Properties of Self-assembled DNA Network on Mica Surface (2009)
  • Author(s): Itsuo HANASAKI, Hirofumi SHINTAKU, Satoshi MATSUNAMI, Satoyuki KAWANO
  • Journal Title: Computer Modeling in Engineering & Sciences Vol.46,No.2 Pages: 191-207
  • Peer Reviewed
• [Journal Article] Droplet Transportation on Vertical Parallel Electrodes Using Electrowetting and Interfacial Oscillation (2009)
  • Author(s): Hirofumi SHINTAKU, Yukio TATARA, Satoyuki KAWANO
  • Journal Title: Journal of Fluid Science and Technology Vol.4,No.3 Pages: 636-647
  • NAID: 110006950958
  • Peer Reviewed
• [Journal Article] Measurements of Electric Field and Electrokinetic Phenomena Using Two Kinds of Tracer Particles with Different Mobilities (2009)
  • Author(s): Hirofumi SHINTAKU, Shuji AZUMA, Satoyuki KAWANO
  • Journal Title: Journal of Fluid Science and Technology Vol.4, No.3 Pages: 687-698
  • Peer Reviewed
• [Journal Article] Droplet Transportation on Vertical Parallel Electrodes Using Electrowetting and Interfacial Oscillation (2009)
  • Author(s): Hirofumi SHINTAKU, Yukio TATARA, Satoyuki KAWANO
  • Journal Title: Journal of Fluid Science and Technology Vol.4, No.3 Pages: 636-647
  • NAID: 110006950958
  • Peer Reviewed
• [Journal Article] Structural and Tensile Properties of Self-assembled DNA Network on Mica Surface (2009)
  • Author(s): Itsuo HANASAKI, Hirofumi SHINTAKU, Satoshi MATSUNAMI, Satoyuki KAWANO
  • Journal Title: Computer Modeling in Engineering & Sciences V6l.46, No.2 Pages: 191-207
  • Peer Reviewed
• [Journal Article] Electrowettingおよび界面振動を用いた鉛直平行電極上における微小液滴の輸送 (2008)
  • Author(s): 新宅博文, 多田羅晋生, 川野聡恭
  • Journal Title: 日本機械学会論文集(B編) 第74巻,745号 Pages: 1942-1950
  • NAID: 110006950958
  • Peer Reviewed
• [Journal Article] Microbubble Formations in MEMS-fabricated Rectangular Channels: A High-speed Observation (2008)
  • Author(s): Hirofumi SHINTAKU, Shosaku IMAMURA, Satoyuki KAWANO
  • Journal Title: Exp. Thermal Fluid Sci. Vol.32 Pages: 1132-1140
  • Peer Reviewed
• [Journal Article] 酸素付加能を有する振動流型人工心臓の開発とその牛血実験 (2008)
  • Author(s): 新宅博文, 米村翼, 磯山隆, 山家智之, 川野聡恭
  • Journal Title: 日本機械学会論文集(B編) 第74巻,第740号 Pages: 862-870
  • NAID: 110006655821
  • Peer Reviewed
• [Journal Article] Microbubble Formations in MEMS-Fabricated Rectangular Channels : A High-Speed Observation (2008)
  • Author(s): Hirofumi Shintaku, Shosaku Imamura, Satovuki Kawano
  • Journal Title: Experimental Thermal and Fluid Science 32 Pages: 1132-1140
  • Peer Reviewed
• [Journal Article] Electrowettingおよび界面振動を用いた鉛直平行電極上における微小液滴の輸送 (2008)
  • Author(s): 新宅博文, 多田羅晋生, 川野聡恭
  • Journal Title: 日本機械学会論文集(B編) 第74巻, 745号 Pages: 1942-1950
  • NAID: 110006950958
  • Peer Reviewed
• [Journal Article] Micro Cell Encapsulation and Its Hydrogel-Beads Production Using Microfluidic Device (2007)
  • Author(s): Hirofumi SHINTAKU, Takeo KUWABARA, Satoyuki KAWANO, Takaaki SUZUKI, Isaku KANNO, Hidetoshi KOTERA
  • Journal Title: Microsyst. Technol. Vol.13 Pages: 951-958
  • Peer Reviewed
• [Journal Article] Micro Cell Encapsulation and Its Hydrogel-Beads Production Using Microfluidic Device (2007)
  • Author(s): Hirofumi SHINTAKU, Takeo KUWABARA, Satoyuki KAWANO, Takaaki SUZUKL. Isaku KANNO, and Hidetoshi KOTERA
  • Journal Title: Microsystem Technologies 13 ・ 8-10 Pages: 951-958
  • Peer Reviewed
• [Journal Article] Microbubble Formations in MEMS-Fabricated Rectangular Channels: A High-Speed Observation
  • Author(s): Hirofunii Shintaku, Shosaku Imaniura, Satoyuki Kawano
  • Journal Title: Experimental Thermal and Fluid Science 掲載予定
  • Peer Reviewed
• [Journal Article] 酸素付加能を有する振動流型人工心臓の開発とその牛血実験
  • Author(s): 新宅博文.米村翼, 磯山隆, 山家智之, 川野聡恭
  • Journal Title: 日本機械学会論文集(B編) 掲載予定
  • NAID: 110006655821
  • Peer Reviewed
• [Presentation] Measurement on Electrophoretic Flow Dynamics of lambdaDNA in Nanochannel (2009)
  • Author(s): Satoshi UEHARA, Hirofumi SHINTAKU, Satoyuki KAWANO
  • Organizer: 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: Singapore
  • Year and Date: 2009-12-21
• [Presentation] Measurement on Electrophoretic Flow Dynamics of lambdaDN A in Nanochannel (2009)
  • Author(s): Satoshi Uehara, Hirofumi Shintaku, Satoyuki Kawano
  • Organizer: 3rd East Asian Pacific Student Workshop on Nano-Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 2009-12-21
• [Presentation] 透明性電極を有する微小流路における界面動電現象の計測 (2009)
  • Author(s): 新宅博文, 川野聡恭
  • Organizer: 流体工学部門講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-07
• [Presentation] Flow dynamics of long DNA under electric field in nanochannel (2009)
  • Author(s): Hirofumi SHINTAKU, Satoshi UEHARA, Satoyuki KAWANO
  • Organizer: The 1st Symposium on Micro-Nano Engineering
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-10-15
• [Presentation] Flow dynamics of long DNA under electric field in nanochannel (2009)
  • Author(s): Hirofumi Shintaku, Satoshi Uehara, Satoyuki Kawano
  • Organizer: The 1st Symposium on Micro-Nano Engineering
  • Place of Presentation: 東京都 船堀
  • Year and Date: 2009-10-15
• [Presentation] Developments of Coarse Graining DNA Models for Single Nucleotide Resolution Analysis (2009)
  • Author(s): Kentaro DOI, Yukihiro TOYOKITA, Tomoaki HAGA, Hirofumi SHINTAKU, Satoyuki KAWANO
  • Organizer: IV International Congress on Computational Bioengineering
  • Place of Presentation: Bertinoro, Italy
  • Year and Date: 2009-09-18
• [Presentation] Developments of Coarse Graining DNA Models for Single Nucleotide Resolution Analysis (2009)
  • Author(s): Kentaro Doi, Yukihiro Toyokita, Tomoaki Haga, Hirofumi Shintaku, Satoyuki Kawano
  • Organizer: International Congress on Computational Bioengineering
  • Place of Presentation: Bertinoro, Italy
  • Year and Date: 2009-09-18
• [Presentation] 透明性微小電極を用いた生体高分子のナノ流動制御 (2009)
  • Author(s): 新宅博文, 川野聡恭
  • Organizer: 日本機械学会2009年度年次大会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-09-15
• [Presentation] ナノ流路における生体高分子流動の可視化観察 (2009)
  • Author(s): 上原聡司, 新宅博文, 川野聡恭
  • Organizer: 日本機械学会2009年度年次大会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-09-15
• [Presentation] ナノ流路における生体高分子流動の可視化観察 (2009)
  • Author(s): 上原聡司, 新宅博文, 川野聡恭
  • Organizer: 日本機械学会 2009年度年次大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2009-09-15
• [Presentation] 透明性微小電極を用いた生体高分子のナノ流動制御 (2009)
  • Author(s): 上原聡司, 新宅博文, 川野聡恭
  • Organizer: 日本機械学会 2009年度年次大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2009-09-15
• [Presentation] 鉛直平行電極を用いた微小液滴輸送法における接触線流動と動的接触角 (2009)
  • Author(s): 新宅博文, 多田羅晋生, 川野聡恭
  • Organizer: 関西支部第84期定時総会講演会
  • Place of Presentation: 東大阪
  • Year and Date: 2009-03-16
• [Presentation] ナノ流路における生体高分子の流動とその電気計測 (2009)
  • Author(s): 上原聡司, 新宅博文, 川野聡恭
  • Organizer: 関西学生会学生員卒業研究発表会
  • Place of Presentation: 東大阪
  • Year and Date: 2009-03-15
• [Presentation] Droplet-basedμTASの液滴駆動における接触線流動 (2009)
  • Author(s): 多田羅晋生, 新宅博文, 川野聡恭
  • Organizer: 第21回バイオエンジニアリング講演会
  • Place of Presentation: 札幌
  • Year and Date: 2009-01-24
• [Presentation] 微小電極を有するナノ流路を用いた生体高分子の電気計測 (2009)
  • Author(s): 新宅博文, 上原聡司, 川野聡恭
  • Organizer: 第21回バイオエンジニアリング講演会
  • Place of Presentation: 札幌
  • Year and Date: 2009-01-23
• [Presentation] Droplet-based μTASの液滴駆動における接触線流動 (2009)
  • Author(s): 多田羅晋生, 新宅博文, 川野聡恭
  • Organizer: 第21回バイオエンジニアリング講演会
  • Place of Presentation: 札幌
• [Presentation] 微小電極を有するナノ流路を用いたい生体高分子の電気設計 (2009)
  • Author(s): 新宅博文, 上原聡司, 川野聡恭
  • Organizer: 第21回バイオエンジニアリング講演会
  • Place of Presentation: 札幌
• [Presentation] Effects of Interfacial Oscillation on Electrowetting-Induced Droplet Transportation Using Vertical Parallel Electrodes (2008)
  • Author(s): Yukio TATARA, Hirofumi SHINTAKU, Satoyuki KAWANO
  • Organizer: The Seventh JSME-KSME Thermal and Fluids Engineering Conference
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-10-15
• [Presentation] Two-color m-PIV for Measurement of Electrokinetic Flow Phenomena in Microchannels (2008)
  • Author(s): Hirofumi SHINTAKU, Shuji AZUMA, Satoyuki KAWANO
  • Organizer: International Conference on Computational & Experimental Engineering and Sciences 2008
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2008-03-17
• [Presentation] 多数の分岐を有する微小流路内における電気浸透流の計測 (2008)
  • Author(s): 東修士, 新宅博文, 川野聡恭
  • Organizer: 日本機械学会関西支部第83期定時総会講演会
  • Place of Presentation: 豊中
  • Year and Date: 2008-03-15
• [Presentation] 多数の分岐を有する微小流路内におる電気浸透の計測 (2008)
  • Author(s): 東修士, 新宅博文.川野聡恭
  • Organizer: 日本機械学会関西支部第83期定時総会講演会
  • Place of Presentation: 豊中
  • Year and Date: 2008-03-15
• [Presentation] 微小流路における界面動電現象と電場分布の計測 (2008)
  • Author(s): 新宅博文, 東修士.川野聡恭
  • Organizer: 第2回非線形テクノサイエンス講演会.
  • Place of Presentation: 吹田
  • Year and Date: 2008-03-14
• [Presentation] 微小流路における界面動電現象と電場分布の計測 (2008)
  • Author(s): 新宅博文, 東修士, 川野聡恭
  • Organizer: 第3回非線形テクノサイエンス講演会
  • Place of Presentation: 吹田
  • Year and Date: 2008-03-03
• [Presentation] Effects of Interfacial Oscillation on Electrowetting-Induced Droplet Transportation Using Vertical Parallel Electrodes. (2008)
  • Author(s): Yukio Tatara, Hirofumi Shintaku, and Satoyuki Kawano
  • Organizer: The Seventh JSME-KSME Thermal and Fluids Engineering Conference
  • Place of Presentation: Sapporo, Japan
• [Presentation] 界面振動とElectrowettingを用いた微小液滴の輸送とその数理モデルの構築 (2007)
  • Author(s): 多田羅晋生, 新宅博文, 川野聡恭
  • Organizer: 日本液体微粒化学会第16回微粒化シンポジウム
  • Place of Presentation: 吹田
  • Year and Date: 2007-12-21
• [Presentation] 界面振動とElectrowettmgを用いた原小液滴の輸送とその数理モデルの構築 (2007)
  • Author(s): 多田羅晋生, 新宅博文, 川野聡恭
  • Organizer: 日本液体微粒化学会第16回微粒化シンポジウム
  • Place of Presentation: 吹田
  • Year and Date: 2007-12-20
• [Presentation] MEMS流体技術による医療応用デバイスの開発 (2007)
  • Author(s): 新宅博文
  • Organizer: 数理医工学の学術基盤創成に向けて(主催:日本応用数理学会数理医学研究部会, 大阪大学基礎工学研究科未来研究ラボシズテム「非線形ダイナミクス研究会」, 大阪大学基礎工学研究科未来研究ラボシステム「ニューロ・ナノテクノロジー学術基盤の創成」, 大阪大学基礎工学研究科機能創成専攻
  • Place of Presentation: 豊中
  • Year and Date: 2007-12-07
• [Presentation] ナノ流路内のイオン輸送における電気二重層と界面電荷の影響 (2007)
  • Author(s): 新宅博文, 川野聡恭
  • Organizer: 日本機械学会流体工学部門講演会
  • Place of Presentation: 東広島
  • Year and Date: 2007-11-18
• [Presentation] ナノ流路内のイオン輸送における電気二重層と界面電荷の影響 (2007)
  • Author(s): 新宅博文, 川野聡恭
  • Organizer: 日本機械学会流体工学部門講演会講演概要集
  • Place of Presentation: 東広島
  • Year and Date: 2007-11-17
• [Presentation] Microbubble Formations in MEMS-Fabricated Rectangular Channels: A High-Speed Observation (2007)
  • Author(s): Hirofumi SHINTAKU, Shosaku IMAMURA, Satoyuki KAWANO
  • Organizer: Proceedings of the 1st International Colloquium on Dynamics, Physics and Chemistry of Bubbles and Gas-Liquid Boundaries
  • Place of Presentation: Niseko, Japan
  • Year and Date: 2007-09-25
• [Presentation] Microbubble Formations in MEMS-Fabricated Rectangular Channels: A High-Speed Observation (2007)
  • Author(s): Hirofumi SHINTAKU, Shyosaku IMAMURA, and Satoyuki KAW ANO
  • Organizer: 1st International Colloquium on Dynamics, Physics and Chemistry of Bubbles and Gas-Liquid Boun daries
  • Place of Presentation: Niseko
  • Year and Date: 2007-09-25
• [Presentation] ナノ流動における電気二重層と界面動電位の特性解析 (2007)
  • Author(s): 新宅博文, 川野聡恭
  • Organizer: 日本機械学会2007年度年次大会
  • Place of Presentation: 吹田
  • Year and Date: 2007-09-11
• [Presentation] ナノ流動における電気-重層と界面動電位の特性解析 (2007)
  • Author(s): 新宅博文, 川野聡恭
  • Organizer: 日本機械学会2007年度年次大会講演論文集(vol. 2)
  • Place of Presentation: 吹田
  • Year and Date: 2007-09-11