Gontrol of intracellular trafficking of water channel aquaporin-2

• Project/Area Number: 19770054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: The University of Tokushima
• Principal Investigator: HASEGAWA Takahiro The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (50447273)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,160,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: アクアポリン2 / 腎臓集合管 / 小胞輸送 / プルダウンアッセイ / 結合タンパク質 / リン酸化

Research Abstract

水チャネルアクアポリン2(aquaporin-2;AQP2)は腎臓集合管の水再吸収を担う重要なタンパク質であり、バソプレシンにより細胞内の貯蔵小胞と細胞膜間で輸送が調節される。AQP2の細胞内輸送機構を解明するために、AQP2のC末端領域に結合するタンパク質をGST融合AQP2によるプルダウンアッセイで探索した。AQP2は約40kDおよび約70kDのタンパク質と結合すること、さらに約40kDのタンパク質の結合はAQP2のリン酸化に依存していることが明らかとなった。

Research Products

• [Journal Article] Trafficking of GFP-AQP5 chimeric proteins conferred with unphosphorylated amino acids at their PKA-target motif ((152)SRRTS) in MDCK-II cells. (2009)
  • Author(s): Karabasil MR, Hasegawa T, Azlina A, Purwanti N, Purevjav J, Yao C, Akamatsu T, Hosoi K.
  • Journal Title: J Med Invest 56 Pages: 55-63
  • NAID: 130004465167
  • Peer Reviewed
• [Journal Article] Inhibition and transcriptional silencing of a subtilisin-like proprotein convertase, PACE4/SPC4, reduces the branching morphogenesis of and AQP5 expression in rat embryonic submandibular gland (2009)
  • Author(s): Akamatsu T, Azlina A, Purwanti N, Karabasil MR, Hasegawa T, Yao C, Hosoi K
  • Journal Title: Dev Biol 325 Pages: 434-443
  • Peer Reviewed
• [Journal Article] Trafficking of GFP-AQP 5 chimeric proteins conferred with unphosphorylated amino acids at their PKA-target motif ((152) SRRTS) in MDCK-II cells (2009)
  • Author(s): Karabasil, MR, et.al.
  • Journal Title: J Med Invest 56 Pages: 55-63
  • Peer Reviewed
• [Journal Article] Degradation of submandibular gland AQP5 by parasympathetic denervation of chorda tympani and its recovery by cevimeline, an M3 muscarinic receptor agonist (2008)
  • Author(s): LiX, Azlina A, Karabasil MR, Purwanti N, Hasegawa T, Yao C, Akamatsu T, Hosoi K
  • Journal Title: Am J Physiol Gastrointest Liver Physiol 295
  • Peer Reviewed
• [Journal Article] Localization and trafficking of aquaporin 2 in the kidney (2008)
  • Author(s): Takata K, Matsuzaki T, Tajika Y, Ablimit A, Hasegawa T
  • Journal Title: Histochem Cell Biol 130 Pages: 197-209
• [Journal Article] Localization and trafficking of aquaporin 2 in the kidney (2008)
  • Author(s): Takata, Kuniaki, et.al.
  • Journal Title: Histochem Cell Biol 130 Pages: 197-209
• [Journal Article] Degradation of submandibular gland AQP5 by parasympathetic denervation of chorda tympani and its recovery by cevimeline, an M3 muscarinic receptor agonist (2008)
  • Author(s): Li, Xuefei, et.al.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol 295 Pages: 112-123
  • Peer Reviewed