Research for the mechanism of flagellar construction in the bacterial cell membrane.

• Project/Area Number: 19770125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: FUKUOKA Hajime Tohoku University, 多元物質科学研究所, 助教 (50447190)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,680,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥2,900,000 (Direct Cost: ¥2,900,000)
• Keywords: 生体膜 / 受容体 / チャンネル / 分子モーター / イメージング / バクテリア / 一分子計測 / イオン

Research Abstract

1.べん毛モーター固定子の共役イオン依存的なモーターへの組込みの発見.
2.大腸菌プロトン駆動型べん毛モーター回転子構成素子のGFP融合タンパク質発現系の構築.
3.機能的モーターにおける回転子構成素子のGFP融合タンパク質の観察系の構築.
4.細胞膜上におけるべん毛構造体の拡散運動の検出および拡散運動解析システムの構築.
5.機能的なべん毛モーターにおける,べん毛モーター回転子構成素子の交換の可視化.

Research Products

• [Journal Article] Sodium-dependent dynamic assembly of membrane complexes in sodium-driven flagellar motors (2009)
  • Author(s): Hajiome Fukuoka, Tomoyuki Wada, Seiji Kojima, Akihiko Ishijima, Michio Homma
  • Journal Title: Molecular Microbiology 71 Pages: 825-835
  • Peer Reviewed
• [Journal Article] Sodium-dependent dynamic assembly of membrane complexes in sodiumdriven flagellar motors (2009)
  • Author(s): Hajime Fukuoka
  • Journal Title: Molecular Microbiology 71 Pages: 825-835
  • Peer Reviewed
• [Presentation] 光学顕微鏡を用いた細菌べん毛モーター1分子の観察および計測 (2009)
  • Author(s): 福岡創,石島秋彦
  • Organizer: 第82回細菌学会
  • Place of Presentation: 日本,名古屋
  • Year and Date: 2009-03-12
• [Presentation] 光学顕微鏡を用いた細菌べん毛モーター1分子の観察および計測 (2009)
  • Author(s): 福岡創
  • Organizer: 第82回細菌学会
  • Place of Presentation: 日本, 名古屋
  • Year and Date: 2009-03-12
• [Presentation] Visualization of exchange of rotor component in functioning bacterial flagellar motor (2009)
  • Author(s): Hajime Fukuoka, Shun Terasawa, Yuichi Inoue, Akihiko Ishijima
  • Organizer: BLAST X
  • Place of Presentation: Mexico, Cuernavaca
  • Year and Date: 2009-01-20
• [Presentation] Visualization of exchange of rotor component in functioning bacterial flaeellar motor (2009)
  • Author(s): Hajime Fukuoka
  • Organizer: BLAST X(Bacterial Locomotion And Signal Trans duction X)
  • Place of Presentation: Mexico, Cuernavaca
  • Year and Date: 2009-01-20
• [Presentation] FRAP analysis for visualization of exchange of rotor component in functional bacterial flagellar motor (2008)
  • Author(s): Hajime Fukuoka, Shun Terasawa, Yuichi Inoue, Takeharu Nagai, Akihiko Ishijima
  • Organizer: 第46回生物物理学会年会
  • Place of Presentation: 日本,福岡
  • Year and Date: 2008-12-05
• [Presentation] FRAP analysis for visualization of exchange of rotor component in funct ional bacterial flagellar motor (2008)
  • Author(s): Hajime Fukuoka
  • Organizer: 第46回生物物理学会年会
  • Place of Presentation: 日本, 福岡
  • Year and Date: 2008-12-05
• [Presentation] Eva-FRAPを用いて細菌べん毛モーター構成素子の交換を検出する (2008)
  • Author(s): 福岡創,井上裕一,本間道夫,石島秋彦
  • Organizer: 第5回東北大バイオサイエンスシンポジウム
  • Place of Presentation: 日本,仙台
  • Year and Date: 2008-05-19
• [Presentation] Eva-FRAPを用いて細菌べん毛モーター構成素子の交換を検出する (2008)
  • Author(s): 福岡創
  • Organizer: 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 日本, 仙台
  • Year and Date: 2008-05-19
• [Presentation] Single molecular labeling of rotor component for detection of rot-or rotation in bacterial flagellar motor (2008)
  • Author(s): Hajime Fukuoka, Yuichi Inoue, Michio Homma, Akihiko Ishijima
  • Organizer: Gordon Research Conference
  • Place of Presentation: USA, Ventura
  • Year and Date: 2008-01-16
• [Presentation] Single molecular labeling of rotor component for detection of rotor rotation in bacterial flagellar motor (2008)
  • Author(s): Hajime Fukuoka
  • Organizer: Gordon Research Conference
  • Place of Presentation: USA, Ventura
• [Presentation] イオン駆動回転モーター構成素子のGFPによる一分子標識 (2007)
  • Author(s): 福岡創,齋藤健太,永井健治,本間道夫,石島秋彦
  • Organizer: 第45回日本生物物理学会
  • Place of Presentation: 日本,横浜
  • Year and Date: 2007-12-23
• [Presentation] イオン駆動回転モーター構成素子のGFPによる一分子標識 (2007)
  • Author(s): 福岡 創
  • Organizer: 日本生物物理学会
  • Place of Presentation: 日本,横浜