| Budget Amount *help |
¥3,990,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Research Abstract |
In general, a functional cell-free protein synthesis for membrane proteins at high yield is difficult. In the present study, we developed the novel cell-free protein synthesis technology that was performed the steroid derived detergent removal, the lipid bilayer formation, and the protein synthesis by the dialysis method at the same time. We obtained the functional Bacteriorhodopsin in a large amount (0.5mg/mL reaction solution), and this yield was 5-25 times higher than previously described preparations. In addition, it succeeded a functional overexpression of the rhodopsin from marine algae and eubacteria that could not be obtained in large amount using the living cell expression system.
|
