Chemical mapping of wood cell walls components by ToF-SIMS

• Project/Area Number: 19780133
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 林産科学・木質工学
• Research Institution: Nagoya University
• Principal Investigator: SAITO Kaori Nagoya University, 大学院・生命農学研究科, 研究員 (60447836)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: 木材化学 / 質量分析 / リグニン / 多糖

Research Abstract

本研究の目的は、飛行時間型二次イオン質量分析(ToF-SIMS)を用いて、顕微レベルでの細胞壁化学成分の分布を明らかにすることである。本研究において、リグニンモノマー分子イオンのイメージングにより、広葉樹カエデにおいて、道管はグアイアシル、木繊維はシリンギルリグニンに富むことを示した。また、リグニンモデル化合物を用いた結果、ToF-SIMSによるリグニンS/G比算出の信頼性が高いことが示唆された。以上の結果により、ToF-SIMSのリグニン分布解析に対する有用性が示された。

Research Products

• [Journal Article] 法隆寺の古材はいつ伐採されたか-TOF-SIMSの古材伐採年推定への適用- (2008)
  • Author(s): 齋藤香織, 光谷拓実, 福島和彦
  • Journal Title: 表面科学 29(12) Pages: 777-781
  • NAID: 10024407889
  • Peer Reviewed
• [Journal Article] TOF-SIMSの植物細胞壁化学への応用 (2008)
  • Author(s): 齋藤香織, 福島和彦
  • Journal Title: 植物化学調節学会誌「植物の生長調節」 43(2) Pages: 156-163
  • NAID: 110007007939
• [Journal Article] Discriminating the indistinguishable sapwood from heartwood in discoloured ancient wood by direct molecular mapping of specific extractives using ToF-SIMS (2008)
  • Author(s): Kaori Saito, Takumi Mitsutani, Takanori Imai, Yasuyuki Matsushita, Kazuhiko Fukushima
  • Journal Title: Analytical Chemistry 80 Pages: 1552-1557
  • Peer Reviewed
• [Journal Article] Chemical differences between sapwood and heartwood of Chamaecyparis obtusa detected by ToF-SIMS (2008)
  • Author(s): Kaori Saito, Takumi Mitsutani, Takanori Imai, Yasuyuki Matsushita, Akira Yamamoto, and Kazuhiko Fukushima
  • Journal Title: Applied Surface Science 255 Pages: 1088-1091
  • Peer Reviewed
• [Journal Article] Application of ToF-SIMS to the study on herartwood formation in Cryptomeria japonica tree (2008)
  • Author(s): Katsushi Kuroda, Takanori Imai, Kaori Saito, Toshiyuki Kato, and Kazuhiko Fukushima
  • Journal Title: Applied Surface Science 255 Pages: 1143-1147
  • Peer Reviewed
• [Journal Article] Mapping of the cationic starch adsorbed on pulp fibers by ToF-SIMS (2008)
  • Author(s): Yasuyuki Matsushita, Ayumi Suzuki, Takuya Sekiguchi, Kaori Saito, Takanori Imai, and Kazuhiko Fukushima
  • Journal Title: Applied Surface Science 252 Pages: 1022-1024
  • Peer Reviewed
• [Journal Article] Simulation of SIMS for monomer and dimer of lignin under the assumption of thermal decomposition using QMD method (2008)
  • Author(s): Kenichi Kato, Kazunaka Endo, Daisuke Matsumoto, Tomonori Ida, Kaori Saito, Kazuhiko Fukushima, and Nobuhiko Kato
  • Journal Title: Applied Surface Science 255 Pages: 1040-1043
  • NAID: 120001019944
  • Peer Reviewed
• [Journal Article] Fragment distribution of thermal decomposition for lignin monomer by QMD calculations using the excited and charged model molecules (2008)
  • Author(s): Kazunaka Endo, Daisuke Matsumoto, Kenichi Kato, Yusuke Takagi, Tomonori Ida, Motohiro Mizuno, Kaori Saito, Kazuhiko Fukushima, and Nobuhiko Kato
  • Journal Title: Applied Surface Science 255 Pages: 1048-1051
  • NAID: 120001019943
  • Peer Reviewed
• [Journal Article] 法隆寺の古材はいつ伐採されたか-TOF-SIMSの古材伐採年推定への適用- (2008)
  • Author(s): 齋藤香織
  • Journal Title: 表面科学 29 Pages: 777-781
  • NAID: 10024407889
  • Peer Reviewed
• [Journal Article] TOF-SIMSの植物細胞壁化学への応用 (2008)
  • Author(s): 齋藤香織
  • Journal Title: 植物の生長調節 43 Pages: 156-163
  • NAID: 110007007939
  • Peer Reviewed
• [Journal Article] Application of ToF-SIMS to the study on herartwood formation in Cryptomeria japonica tree (2008)
  • Author(s): 黒田克史
  • Journal Title: Applied Surface Science 255 Pages: 1143-1147
  • Peer Reviewed
• [Journal Article] Discriminating the indistinguishable sapwood from heartwood in discoloured ancient wood by direct molecular mapping of specific extractives using ToF-SIMS (2008)
  • Author(s): 齋藤香織
  • Journal Title: Analytical Chemistry 80 Pages: 1552-1557
  • Peer Reviewed
• [Journal Article] Chemical differences between sapwood and heartwood of Chamaecyparis obtusa detected by ToF-SIMS (2008)
  • Author(s): 齋藤香織
  • Journal Title: Applied Surface Science (in press)
  • Peer Reviewed
• [Journal Article] ToF-SIMS(飛行時間型二次イオン質量分析)による木材化学研究 (2007)
  • Author(s): 福島和彦, 齋藤香織
  • Journal Title: 木材学会誌 53(6) Pages: 291-297
  • NAID: 10029786447
  • Peer Reviewed
• [Journal Article] TOF-SIMS imaging of lignin after the removal of polysaccharides by periodate oxidation followed by hydrolysis in xylem of Pinus thunbergii (2007)
  • Author(s): Kaori Saito, Noritsugu Terashima, Takanori Imai, Yasuyuki Matsushita and Kazuhiko Fukushima
  • Journal Title: Cellulose Chemistry and Technology 2007 Pages: 583-589
  • Peer Reviewed
• [Journal Article] The characteristic fragment ions and visualization of cationic starches on pulp fiber using ToF-SIMS. (2007)
  • Author(s): Yasuyuki Matsusita, Takuya Sekiguchi, Kaori Saito, Takanori Imai and Kazuhiko Fukushima
  • Journal Title: Surface and Interface Analysis, 39(6) Pages: 501-505
  • Peer Reviewed
• [Journal Article] ToF-SIMS(飛行時間型二次イオン質量分析)による木材化学研究 (2007)
  • Author(s): 福島和彦、齋藤香織
  • Journal Title: 木材学会誌 53 Pages: 291-197
  • NAID: 10029786447
  • Peer Reviewed
• [Presentation] TOF-SIMS によるヒノキ心材抽出成分の分子マッピング (2009)
  • Author(s): 齋藤香織、吉田正人、今井貴規、松下泰幸、福島和彦
  • Organizer: 第59回日本木材学会大会
  • Place of Presentation: 松本大学(松本)
• [Presentation] TOF-SIMSによる植物バイオマスの分析 (2008)
  • Author(s): 齋藤香織
  • Organizer: 第56回質量分析総合討論会
  • Place of Presentation: つくば(茨城県)
  • Year and Date: 2008-05-14
• [Presentation] ToF-SIMSによるTOF-SIMSによるリグニンS/G比顕微分析法の開発 (2008)
  • Author(s): 齋藤香織、渡邊陽子、岸本崇生、家田真理子、勝山さやか、松下泰幸、今井貴規、船田良、小池孝良、深澤和三、福島和彦
  • Organizer: 第53回リグニン討論会
  • Place of Presentation: 東京大学(東京)
• [Presentation] Discriminating the indistinguishable sapwood from heartwood in discoloured ancient wood by direct molecular mapping of specific extractives using TOF-SIMS (2008)
  • Author(s): K. Saito, T. Mitsutani, T. Imai, Y. Matsushita, K. Fukushima
  • Organizer: IAWPS2008(International Symposium on Wood Science and Technology)
  • Place of Presentation: Harbin, China
• [Presentation] Direct molecular imaging of heartwood compounds in Hinoki cypress using TOF-SIMS (2008)
  • Author(s): K. Saito, T. Imai, Y. Matsushita, K. Fukushima
  • Organizer: ICP2008 (XXIVth International Conference on Polyphenols)
  • Place of Presentation: Salamanca, Spain
• [Presentation] TOF-SIMSによる植物バイオマスの分析 (2008)
  • Author(s): 齋藤香織
  • Organizer: 第56回質量分析総合討論会
  • Place of Presentation: つくば(東京)
• [Presentation] The chemical analysis of plant cell wall using ToF-SIMS (2008)
  • Author(s): K. Fukushima and K. Saito
  • Organizer: 8th International Symposium on Biomimetic Materials
  • Place of Presentation: Nagoya, Japan
• [Presentation] ToF-SIMSによる広葉樹木部におけるリグニン分布の解析 (2007)
  • Author(s): 齋藤香織
  • Organizer: 第52回リグニン討論会
  • Place of Presentation: 宇都宮大学(茨城県宇都宮)
  • Year and Date: 2007-11-14
• [Presentation] Visualization of heartwood extractives using ToF-SIMS (2007)
  • Author(s): K. Saito, T. Imai, Y. Matsushita, T. Mitsutani, and K. Fukushima
  • Organizer: 14th International Symposium on Wood, Fiber and Pulping Chemistry
  • Place of Presentation: Durban, South Africa
• [Presentation] ToF-SIMSによる広葉樹木部におけるリグニン分布の解析 (2007)
  • Author(s): 齋藤香織、渡邊陽子、勝山さやか、松下泰幸、今井貴規、船田良、小池孝良、深澤和三、福島和彦
  • Organizer: 第52回リグニン討論会
  • Place of Presentation: 宇都宮大学(宇都宮)
• [Presentation] ToF-SIMSによるヒノキ心材抽出成分のケミカルマッピング (2007)
  • Author(s): 齋藤香織、今井貴規、松下泰幸、福島和彦
  • Organizer: 第57回日本木材学会大会
  • Place of Presentation: 安田女子大学(広島)
• [Presentation] ヒノキ古材におけるToF-SIMSを用いた辺材と心材の識別 (2007)
  • Author(s): 齋藤香織、光谷拓実、松下泰幸、今井貴規、福島和彦
  • Organizer: 第74回紙パルプ研究発表会
  • Place of Presentation: 船堀(東京)
• [Remarks] 新聞掲載 : 福島和彦・齋藤香織ら、ToF-SIMSを用いて、古代の木造建築に使われた建材の伐採年代を測定する技術を開発、2008年3月15日掲載、読売新聞、中日新聞、毎日新聞各朝刊(論文(1) Analytical Chemistry,80, 1552(2008)の発表に伴う。
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~lignin/