The role of decorin in the process of musculoskeletal differentiation from mesenchymal stem cells

• Project/Area Number: 19780220
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Tottori University (2008); Rakuno Gakuen University (2007)
• Principal Investigator: HOSAKA Yoshinao Tottori University, 農学部, 准教授 (00337023)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 間葉系幹細胞 / 分化と増殖 / 細胞系譜の決定 / デコリン / 骨 / 脂肪 / ジーンサイレンシング / siRNA / ノックダウン / 運動器 / 分化の誘導と抑制

Research Abstract

マウス間葉系幹細胞KUSA-A1(A1)のデコリン発現をノックダウンした細胞株(siDT)を用いて、骨分化過程でのデコリンの関与と機能を解析した。siDTを骨芽細胞分化培地で誘導しても骨芽細胞へ分化せずに脂肪細胞へと分化した。A1およびsiDTをディフュージョンチャンバーに充填してマウス腹腔内に、あるいは、単独またはコラーゲンゲルとともにマウス皮下に移植して異所性骨化を生体内へ移植したところ、A1は腹腔内あるいは皮下で骨芽細胞へと分化したが、siDTは骨分化マーカーの産生が低いことや、組織学的解析結果と総合して骨分化は起きていないと判断した。以上の結果は、デコリンが間葉系幹細胞の骨分化への方向付け因子であることを強く示唆する。

Research Products

• [Journal Article] Modulation of collagen fibril assembly and its structure by decorin: An electron microscopic study (2008)
  • Author(s): Iwasaki S, Hosaka Y, Iwasaki T, Yamamoto K, Nagayasu A, Ueda H, Kokai Y,Takehana K.
  • Journal Title: Arch Histol Cytol 71 Pages: 37-44
  • NAID: 10021998631
• [Journal Article] The role of decorin in the process of osteoblastic differentiation from mouse mesenchymal stem cell, KUSA-A1 (2008)
  • Author(s): Hosaka Y, Kokai Y, Hayashi H, Takehana K, Imagawa T, Uehara M.
  • Journal Title: Proceedings of 9th of International Congress on Cell Biology Pages: 104-104
• [Journal Article] Heparan sulfate promotes human mesenchymal stem cell growth and viability in vitro and stimulates bone repair in vivo (2008)
  • Author(s): Sawyer A, Lim XH, Hosaka Y, Rider D, Poh WT, Hui J, Doshi H, Nurcombe V, Cool S,
  • Journal Title: Proceeding of The 8th World Biomaterials Congress Pages: 52-52
• [Journal Article] Modulation of collagen fibril assembly and its structure by decorin : Anelectron microscopic study (2008)
  • Author(s): Iwasaki S
  • Journal Title: Arch Histol Cytol 71 Pages: 37-44
  • Peer Reviewed
• [Journal Article] A preliminary study of direct application of atelocollagen into a wound lesion in the doa cornea (2008)
  • Author(s): Nagayasu A
  • Journal Title: Current Eye Res 33 Pages: 727-735
  • Peer Reviewed
• [Journal Article] The role of decorin in the process of osteoblastic differentiation from mouse mesenchymal stem cell, KUSA-Al (2008)
  • Author(s): Hosaka Y
  • Journal Title: Proceedings of the 9^<th> of International Congress on Cell Biology 9 Pages: 104-104
  • Peer Reviewed
• [Journal Article] Heparan sulfate promotes human mesenchymal stem cell growth and viability in vitro and stimulates bone renair in vivo (2008)
  • Author(s): Sawyer A
  • Journal Title: Proceeding of the 8^<th> World Biomaterials Congress 8 Pages: 52-52
  • Peer Reviewed
• [Journal Article] Decorin modulates collagen fibril assembly and its structure; electron microscopic study (2008)
  • Author(s): Iwasaki S, Hosaka Y, et al
  • Journal Title: Archives of Histology and Cytology (In press)
  • Peer Reviewed
• [Journal Article] Passively adsorbed heparan sulfate maintains bioactivity and promotes human mesenchymal stem cell growth and viability within collagen scaffolds
  • Author(s): Sawyer A, Lim XH, Susanro E, Hosaka Y, Rider D, Poh WT, Hutmacher DW, Nurcombe V, Cool SM,
  • Journal Title: Proceedings of ICCMB 2007 Pages: 2007-2007
• [Presentation] The role of decorin in the process of osteoblastic differentiation from mesenchymal stem cells (2008)
  • Author(s): Hosaka Y
  • Organizer: The 9^<th> of International Congress on Cell Biology
  • Place of Presentation: 大韓民国ソウルCOEX
  • Year and Date: 2008-10-29
• [Presentation] 間葉系幹細胞から骨芽細胞への分化過程でのデコリンの関与と役割 (2008)
  • Author(s): 保坂善真、小海康夫、今川智敬、上原正人
  • Organizer: 第63回日本解剖学会中国・四国地方会
  • Place of Presentation: 出雲
  • Year and Date: 2008-10-25
• [Presentation] 問葉系幹細胞から骨へ分化する過程でのデコリンの関与と役割 (2008)
  • Author(s): 保坂善真
  • Organizer: 第63回中国・四国支部学術集会
  • Place of Presentation: 島根大学医学部
  • Year and Date: 2008-10-25
• [Presentation] The role of decorin in the process of osteoblastic differentiation from mesenchymal stem cells (2008)
  • Author(s): Hosaka Y., Kokai Y., Hayashi H., Takehana K., Imagawa T., Uehara M.
  • Organizer: 9th of International Congress on Cell Biology
  • Place of Presentation: ソウル,韓国
  • Year and Date: 2008-10-08
• [Presentation] 間葉系幹細胞から骨へ分化する過程でのデコリンの役割と機能 (2008)
  • Author(s): 保坂善真、小海康夫、今川智敬、上原正人
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-09-25
• [Presentation] 間葉系幹細胞から骨へ分化する過程でのデコリンの役割と機能 (2008)
  • Author(s): 保坂善真
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎シーガイア
  • Year and Date: 2008-09-25
• [Presentation] Heparan sulfate promotes human mesenchymal stem cell growth and viability in vitro and stimulates bone repair in vivo (2008)
  • Author(s): Sawyer A, Lim XH, Hosaka Y, Rider D, Poh WT, Hui J, Doshi H, Nurcombe V, Cool S,
  • Place of Presentation: アムステルダム, オランダ
  • Year and Date: 2008-05-30
• [Presentation] Heparan sulfate promotes human mesenchymal stem cell growth and viability in vitro and stimulates bone repair in vivo (2008)
  • Author(s): Sawyer A
  • Organizer: The 8^<th> World Biomaterials Congress
  • Place of Presentation: オランダアムステルダムRAI Congress Centre
  • Year and Date: 2008-05-29
• [Presentation] Passively adsorbed heparan sulfate maintains bioactivity and promotes human mesenchymal stem cell growth and viability within collagen scaffolds (2007)
  • Author(s): Sawyer A, Lim XH, Susanro E., Hosaka Y., Rider D, Poh WT, Hutmacher DW., Nurcombe V, Cool SM,
  • Organizer: ICCMB 2007
  • Place of Presentation: シンガポール
  • Year and Date: 2007-12-11
• [Presentation] Analysis of interaction in decorin and collagen assembly. (2007)
  • Author(s): Hosaka Y, et. al.
  • Organizer: The 2nd meeting of Asian Anatomist of Veterinary Association
  • Place of Presentation: タイ王国バンコク
  • Year and Date: 2007-09-13
• [Remarks] ホームページ等
  • URL: http://muses.muses.tottori-u.ac.jp/staff/y-hosa.htm
• [Remarks]
  • URL: http://muses.muses.tottori-u.ac.jp/staff/y-hosa.htm