| Budget Amount *help |
¥3,730,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
The effect of intramammary infusion of recombinant bovine (rb) cytokine on mononuclear cell populations in udders, and CMT score was investigated. Twenty-one cows with naturally occurring subclinical mastitis were infused rbGM-CSF, rbIL-8 and the combination of the two into the udders. Milk samples were collected from the udders on days 0, 1/4, 1, 2, 3, 7 and 14 after infusions of all cytokines. The CMT score sharply rose between days 1/4 and 2, and was followed by a smooth decline to low pre-infusion levels between days 7 and 14. Interestingly, the percentage of CD14+ cells increased on days 1 and 2 after infusion of rbGM-CSF only. Subsequent to this increase in CD14+ cells, CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentages of CD4+ and CD8+ cells decreased on days 1/4 and 1 after infusion of rbIL-8 and the combination respectively. However, the mechanism of this decrease following the infusion of the two is not understood and this calls for future work in this area. These results demonstrate that the intramammary infusion of rbGM-CSF induces the alteration of mononuclear cell populations in milk, and that it may have potential as a therapeutic agent for subclinical mastitis of dairy cows.
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