Physical and functional interaction among nectin, integrin, and platelet-derived growth factor

• Project/Area Number: 19790230
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Kobe University (2008); Osaka University (2007)
• Principal Investigator: OGITA Hisakazu Kobe University, 大学院・医学研究科, 准教授 (50379236)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 接着分子 / 細胞生存 / シグナル伝達 / ネクチン / インテグリン / 増殖因子受容体 / 細胞運動 / 細胞極性 / 細胞間接着 / PI3-キナーゼ / Akt

Research Abstract

新規の免疫グロブリン様接着分子ネクチンは細胞間接着の形成に重要であるが、その際、細胞-基質間接着分子インテグリンαvβ3の活性化が必要であった。細胞間接着形成後、ネクチンの作用によりインテグリンαvβ3は不活性化された。このことは細胞間接着の維持に重要であった。また、細胞間接着形成後も細胞が生存するために、ネクチンは血小板由来増殖因子受容体とも相互作用し、ネクチンの裏打ちタンパク質であるアファディンと共に、ホスファチジルイノシトール3-キナーゼ-Akt系による生存シグナルを亢進した。

Research Products

• [Journal Article] Roles of cell adhesion molecules nectin and nectin-like molecule-5 in the regulation of cell movement and proliferation (2008)
  • Author(s): Ogita H., Ikeda W., Takai Y.
  • Journal Title: J Microsc 231 Pages: 455-465
  • Peer Reviewed
• [Journal Article] Nectins and nectin-like molecules : roles in contact inhibition of cell movement and proliferation (2008)
  • Author(s): Takai Y., Miyoshi J., Ikeda W., Ogita H.
  • Journal Title: Nat Rev Mol Cell Biol 9 Pages: 603-615
  • Peer Reviewed
• [Journal Article] Involvement of the nectin-afadin complex in PDGF-induced cell survival (2008)
  • Author(s): Kanzaki N., Ogita H., Komura H., Ozaki M., Sakamoto Y., Majima T., Ijuin T., Takenawa T., Takai Y.
  • Journal Title: J Cell Sci 121 Pages: 2008-2017
  • Peer Reviewed
• [Journal Article] Establishment of cell polarity by afadin during the formation of embryoid bodies (2008)
  • Author(s): Komura H., Ogita H., Ikeda W., Mizoguchi A., Miyoshi J., Takai Y.
  • Journal Title: Genes Cells 13 Pages: 79-90
  • Peer Reviewed
• [Journal Article] Involvement of Nectin in Inactivation of Integrin αvβ3 after the Establishment of Cell-Cell Adhesion (2008)
  • Author(s): Sakamoto Y., Ogita H., Komura H., Takai Y.
  • Journal Title: J Biol Chem 283 Pages: 496-505
  • Peer Reviewed
• [Journal Article] Involvement of the nectin-afadin complex in PDGF-induced cell survival (2008)
  • Author(s): Kanzaki, N., et.al.
  • Journal Title: J. Cell Sci. 121巻・12号 Pages: 2008-2017
  • Peer Reviewed
• [Journal Article] Roles of cell adhesion molecules nectin and nectin-like molecule-5 in the regulation of cell movement and uroliferation (2008)
  • Author(s): Ogita, H., et.al.
  • Journal Title: J. Microsc 231巻・3号 Pages: 455-465
  • Peer Reviewed
• [Journal Article] Nectins and nectin-like molecules : roles in contact inhibition of cell movement and proliferation (2008)
  • Author(s): Takai, Y., et.al.
  • Journal Title: Nat. Rev. Mol. Cell. Biol 9巻・8号 Pages: 603-615
  • Peer Reviewed
• [Journal Article] Establishment of cell polarity by afadin during the formation of embrvoid bodies. (2008)
  • Author(s): Komura, H.
  • Journal Title: Genes Cells 13 Pages: 79-90
  • Peer Reviewed
• [Journal Article] Involvement of nectin in inactivation of integrin αvβ3 after the establishment of cell-cell adhesion. (2008)
  • Author(s): Sakamoto, Y.
  • Journal Title: J Biol Chem 283 Pages: 496-505
  • Peer Reviewed
• [Journal Article] Regulation of Platelet-derived Growth Factor Receptor Activation by Afadin through SHP-2 : Implications for Cellular Morphology (2007)
  • Author(s): Nakata S., Fujita N., Kitagawa Y., Okamoto R., Ogita H., Takai Y.
  • Journal Title: J Biol Chem 282 Pages: 37815-37825
  • Peer Reviewed
• [Journal Article] The roles of nectins in cell adhesions : cooperation with other cell adhesion molecules and growth factor receptors (2007)
  • Author(s): Sakisaka T., Ikeda W., Ogita H., Fujita N., Takai Y.
  • Journal Title: Curr Opin Cell Biol 19 Pages: 593-602
  • Peer Reviewed
• [Journal Article] Inhibition of apoptosis-regulated signaling kinase-1 and prevention of congestive heart failure by estrogen (2007)
  • Author(s): Satoh M., Matter C. M., Ogita H., Takeshita K., Wang C. Y., Dorn G. W., Liao J. K.
  • Journal Title: Circulation 115 Pages: 3197-3204
  • Peer Reviewed
• [Journal Article] Up-regulation of loricrin expression by cell adhesion molecule nectin-1 through Rap1-ERK signaling in keratinocytes (2007)
  • Author(s): Wakamatsu K., Ogita H., Okabe N., Irie K., Tanaka-Okamoto M., Ishizaki H., Ishida-Yamamoto A., Iizuka H., Miyoshi J., Takai Y.
  • Journal Title: J Biol Chem 282 Pages: 18173-18181
  • Peer Reviewed
• [Journal Article] Regulation of platelet-derived growth factor receptor activation by afadin through SHP-2: implications for cellular morphology. (2007)
  • Author(s): Nakata, S.
  • Journal Title: J Biol Chem 282 Pages: 37815-37825
  • Peer Reviewed
• [Journal Article] The roles of nectins in cell adhesions: cooperation with other cell adhesion molecules and growth factor receptors. (2007)
  • Author(s): Sakisaka, T.
  • Journal Title: Curr Opin Cell Biol 19 Pages: 593-602
  • Peer Reviewed
• [Journal Article] Involvement of integrin-induced activation of protein kinase C in the formation of adherens junctions. (2007)
  • Author(s): Ozaki, M.
  • Journal Title: Genes Cells 12 Pages: 651-662
  • Peer Reviewed
• [Journal Article] Up-regulation of loricrin expression by cell adhesion molecule nectin- 1 through Rap 1-ERK signaling in keratinocytes. (2007)
  • Author(s): Wakamatsu, K.
  • Journal Title: J Biol Chem 282 Pages: 18173-18181
  • Peer Reviewed
• [Journal Article] Cross-talk among integrin, cadherin, and growth factor receptor: roles of nectin and nectin-like molecule.
  • Author(s): Ogita, H.
  • Journal Title: Int Rev Cytol in press
  • Peer Reviewed
• [Journal Article] Roles of cell adhesion molecules nectin and nectin-like molecule-5 in the regulation of cell movement and proliferation.
  • Author(s): Ogita, H.
  • Journal Title: J Microsc in press
  • Peer Reviewed
• [Presentation] 組織形成における接着分子ネクチン-アファディン系の役割 (2008)
  • Author(s): 扇田久和, 高井義美
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] がん化による細胞接着・運動・増殖異常とその分子機構 (2008)
  • Author(s): 扇田久和, 高井義美
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] 細胞間接着におけるネクチン、インテグリンおよび血小板由来増殖因子受容体間の相互作用 (2007)
  • Author(s): 扇田久和, 高井義美
  • Organizer: 第30回日本分子生物学会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 細胞間接着におけるネクチン、インテグリンおよび血小板由来増殖因子受容体間の相互作用 (2007)
  • Author(s): 扇田 久和
  • Organizer: 日本分子生物学会・日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 細胞間接着形成におけるネクチンとインテグリンのクロストーク機構 (2007)
  • Author(s): 扇田久和, 高井義美
  • Organizer: 第40回日本発生生物学会・第59回日本細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] 細胞間接着形成におけるネクチンとインテグリンのクロストーク機構 (2007)
  • Author(s): 扇田 久和
  • Organizer: 日本発生生物学会・日本細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Book] The Sticky Synapse (2009)
  • Author(s): Togashi H., Ogita H., Takai Y.
  • Publisher: Springer(in press)
• [Book] Annu Rev Cell Dev Biol (2008)
  • Author(s): Takai Y., Ikeda W., Ogita H., Rikitake Y.
  • Publisher: Annual Review
• [Book] Int Rev Cytol (2008)
  • Author(s): Ogita H., Takai Y.
  • Publisher: Elsevier Inc.
• [Book] Int. Rev. Cytol (2008)
  • Author(s): Ogita, H. and Takai, Y.
  • Publisher: Elsevier Inc
• [Book] Annu. Rev. Cell Dev. Biol (2008)
  • Author(s): Takai, Y., et.al.
  • Publisher: Annual Review
• [Book] The Sticky Synapse(in press) (2008)
  • Author(s): Togashi, H., et.al
  • Publisher: Springer
• [Remarks] ホームページ
  • URL: http://www.med.kobe-u.ac.jp/mcb/index.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/mcb/index.html