Analysis of peripheral blood hematopoietic stemness using human hTERT-stromal cells

• Project/Area Number: 19790672
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Sapporo Medical University
• Principal Investigator: KAWANO Yutaka Sapporo Medical University, 医学部, 助教 (80398320)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 末梢血 / 造血幹細胞 / CD133 / 骨髄間質細胞 / hTERT

Research Abstract

ヒトテロメレース遺伝子を導入したストローマ(以下HTS)との共培養により末梢血CD133陽性細胞は臍帯血CD34陽性細胞と同等に血液細胞の増幅が可能であった.末梢血CD133陽性細胞をHTS接触下あるいは非接触下で共培養を行い血液細胞の増幅に差を認めなかった.液性因子であるSCF、angiopoietin、およびWnt抑制因子を添加しながらHTSと共培養をおこなった.その結果、上記因子の添加有無で血液細胞の増幅や細胞周期へのentryは同等であった.

Research Products

• [Journal Article] Adenoviral vector-mediated transfer of the Indian hedgehog gene modulates lymphomyelopoiesis in vivo. (2008)
  • Author(s): Kobune M, Kato J, Kawano Y, Sasaki K, Uchida H, Takada K, Takahashi S, Takimoto R, Niitsu Y.
  • Journal Title: Stem Cells. 6(2) Pages: 534-542
  • Peer Reviewed
• [Journal Article] Adenoviral vector-mediated transfer of the Indian hedgehog gene modulates lymphomyelopoiesis in vivo (2008)
  • Author(s): Kobune M, et.al.
  • Journal Title: Stem Cells 6 Pages: 534-542
  • Peer Reviewed
• [Journal Article] Adenoviral vector-mediated transfer of the Indian hedgehog gene modulates lymphomyelopoiesis in vivo (2008)
  • Author(s): Kobune M, et. al.
  • Journal Title: Stem Cells 26 Pages: 534-542
  • Peer Reviewed
• [Journal Article] Wnt3/RhoA/ROCK signaling pathway is involved in adhesion-mediated drug resistance of multiple myeloma in an autocrine mechanism. (2007)
  • Author(s): Kobune M, Chiba H, Kato J, Kato K, Nakamura K, Kawano Y, Takada K, Takimoto R, Takayama T, Hamada H, Niitsu Y.
  • Journal Title: Mol Cancer Ther. 6(6) Pages: 1774-1784
  • Peer Reviewed
• [Journal Article] Ex vivo expansion of G-CSF-mobilized peripheral blood CD133+ progenitor cells on coculture with human stromal cells. (2006)
  • Author(s): Kawano Y, Kobune M, Chiba H, Nakamura K, Takimoto R, Takada K, Ito Y, Kato J, Hamada H, Niitsu Y.
  • Journal Title: Exp Hematol 34(2) Pages: 150-158
  • Peer Reviewed
• [Journal Article] Ex vivo expansion of G-CSF-mobilized peripheral blood CD133+ progenit or cells on coculture with human stromal cells (2006)
  • Author(s): Kawano Y, et. al.
  • Journal Title: Exp Hematol 34 Pages: 150-158
  • Peer Reviewed
• [Journal Article] Wnt3/RhoA/ROCK signaling pathway is involved in adhesion-mediated drug resistance of multiple myeloma in an autocrine mechanism (2006)
  • Author(s): Kobune M, et. al.
  • Journal Title: Mol Cancer Ther 6 Pages: 1774-1784
  • Peer Reviewed