Regulation of osteoblast differentiation by interaction between Notch and Wnt/beta-catenin signal

• Project/Area Number: 19791340
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAMAMURA Yoshihiro Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 特任助教 (70431963)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 骨芽細胞 / Notch / Wnt / βcatenin

Research Abstract

骨髄由来細胞株を用いた実験から、Nocthシグナルは、Nocth細胞内ドメインがβcateninと複合体を形成し、Wnt/βcateninシグナル標的遺伝子の転写を抑制することにより骨芽細胞分化を抑制することが明らかとなった。さらに骨芽細胞特異的にNocthシグナルを活性化したマウスでは、骨形成が低下し、Wnt/βcateninシグナル標的遺伝子の発現が減少していた。以上の結果より、Nocthシグナルは、Wnt/βcateninシグナルを抑制することにより、骨芽細胞分化を調節する役割を持つことが示唆された。

Research Products

• [Journal Article] Role of CCN3, a vertebrate specific gene family, in development (2009)
  • Author(s): 勝部憲一、坂本啓、玉村禎宏, (他1名)
  • Journal Title: Development, growth and differentiation 51 Pages: 55-67
  • Peer Reviewed
• [Journal Article] Role of CCN, a vertebrate specific gene family, in development (2009)
  • Author(s): Katsube K, Sakamoto K, Tamamura Y, et.al.
  • Journal Title: Development, growth & differentiation 51 Pages: 55-67
  • NAID: 10023963730
• [Journal Article] Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells (2008)
  • Author(s): 坂本啓、玉村禎宏、勝部憲一, (他1名)
  • Journal Title: Journal of cell science 121 Pages: 1613-23
  • Peer Reviewed
• [Journal Article] Wnt/beta-catenin signaling regulates cranial basedevelopment and growth (2008)
  • Author(s): Nagayama M., Iwamoto ., Hargett A., (他8名、5番目)
  • Journal Title: Journal of dental research 87 Pages: 244-9
  • Peer Reviewed
• [Journal Article] Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells (2008)
  • Author(s): Sakamoto K, Tamamura Y, Katsube K, et.al.
  • Journal Title: Journal of Cell Science 121 Pages: 1613-23
• [Journal Article] Wnt/beta-catenin signaling regulates cranial base development and growth (2008)
  • Author(s): Nagayama M, Iwamoto M, Hargett A, Kamiya N, Tamamura Y, et. al.
  • Journal Title: Journal of Dental Research 87 Pages: 244-9
• [Journal Article] Transcription factor ERG and joint and articular cartilage formation during mouse limb and spine skeletogenesis (2007)
  • Author(s): Iwamoto M, Tamamura Y, Koyama E, et. al.
  • Journal Title: Developmental Biology 305 Pages: 40-51
• [Presentation] NotchシグナルとWnt/βカテニンシグナルの相互作用による骨芽細胞分化調節機構 (2007)
  • Author(s): 玉村禎宏
  • Organizer: 第25回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-07-19