Proteins for oxidation of elemental sulfur from a sulfur-oxidizing bacterium in deep-sea hydrothermal fields

• Project/Area Number: 19870039
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Ecology/Environment
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: YAMAMOTO Masahiro Japan Agency for Marine-Earth Science and Technology, 極限環境生物圏研究センター, 研究員 (60435849)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,011,000 (Direct Cost: ¥2,630,000、Indirect Cost: ¥381,000); Fiscal Year 2008: ¥1,651,000 (Direct Cost: ¥1,270,000、Indirect Cost: ¥381,000); Fiscal Year 2007: ¥1,360,000 (Direct Cost: ¥1,360,000)
• Keywords: 硫黄代謝 / 酵素 / 微生物学 / 海洋生態

Research Abstract

深海熱水環境の優占微生物であるイプシロンプロテオバクテリアの硫黄代謝経路について種々の解析を行い、この菌が複数の硫黄代謝経路を同時に利用していることを明らかにした。
これらの代謝の鍵酵素の基質はポリスルフィドなどの水溶性化合物であるため不溶性の元素状硫黄に直接作用するタンパク質をさらに追跡したところスルフィドキノン還元酵素(SQR)様タンパク質が精製された。
水に不溶な元素状硫黄を効率的に代謝径路に取り込み、そこからエネルギーを取り出すモデルを構築できた。

Research Products

• [Journal Article] エネルギー代謝の進化から見た初期生命進化--ltraH3 リンケージ仮説 (2007)
  • Author(s): 高井研、中村謙太郎、宮崎淳一、山本正浩、鈴木勝彦、熊谷英憲
  • Journal Title: 生物の科学 遺伝 Vol. 61 No. 6 Pages: 28-36
• [Journal Article] Sequencing and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis of Four Hydrogenase Gene Clusters from an Obligately Autotrophic Hydrogen-Oxidizing Bacterium Hydrogenobacter thermophilus TK-6 (2007)
  • Author(s): Ueda, Y., Yamamoto, M., Urasaki, T., Arai, H., Ishii, M. and Igarashi, Y
  • Journal Title: J. Biosci. Bioeng Vol. 104 Pages: 470-475
  • NAID: 110006533082
  • Peer Reviewed
• [Presentation] 深海熱水域におけるタンパク質の直接採集と酵素解析 (2009)
  • Author(s): 山本正浩
  • Organizer: Blue Earth'09
  • Place of Presentation: 立教大学、東京
  • Year and Date: 2009-03-13
• [Presentation] 深海熱水域におけるタンパク質の直接採集と酵素解析 (2009)
  • Author(s): 山本正浩
  • Organizer: Blue Earth'09
  • Place of Presentation: 東京(立教大学)
  • Year and Date: 2009-03-13
• [Presentation] Enzymatic Analyses ofMicrobial Energy Metabolism Using Proteins Directly Extracted from Deep-Sea Hydrothermal Environments (2008)
  • Author(s): Yamamoto, M
  • Organizer: 7~<th> International Symposium for Subsurface Microbiology
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 2008-11-17
• [Presentation] Enzymatic Analyses of Microbial Energy Metabolism UsingProteins Directly Extracted from Deep-Sea Hydrothermal Environments (2008)
  • Author(s): Masahiro Yamamoto
  • Organizer: 7th International Symposium for Subsurface Microbiology
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 2008-11-17
• [Presentation] Microbial Oxidation of Inorganic Sulfur Compounds in Deep-Sea Hydrothermal Field. -Cases for Epsilon-Proteobacteria- (2008)
  • Author(s): Yamamoto, M
  • Organizer: International Symposium on Environmental Microbiology
  • Place of Presentation: Tokyo Metropolitan University, Tokyo
  • Year and Date: 2008-02-29
• [Presentation] Microbial oxidation of inorganic sulfur compounds in deep-sea hydrothe rmal field:cases for epsilon-Proteobacteria (2008)
  • Author(s): 山本 正浩
  • Organizer: International Symposium on Environment Biology
  • Place of Presentation: Tokyo Metropolitan University
  • Year and Date: 2008-02-29
• [Presentation] 硫黄酸化の熱水生態系における重要性 (2007)
  • Author(s): 山本正浩
  • Organizer: InterRidge-Japan 研究発表集会
  • Place of Presentation: 東京大学海洋研究所、東京
  • Year and Date: 2007-10-30
• [Presentation] 硫黄酸化の熱水生態系における重要性 (2007)
  • Author(s): 山本 正浩
  • Organizer: InterRidge-Japan研究発表集会
  • Place of Presentation: 東京大学海洋研究所
  • Year and Date: 2007-10-30
• [Presentation] Enzymatic Characterization of Thiosulfate Oxidation in Epsilon-Proteobacteria Isolated from Deep-Sea Hydrothermal Field (2007)
  • Author(s): Yamamoto, M.
  • Organizer: InterRidge Theoretical Institute 'Biogeochemical interaction at deep-sea vents
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2007-09-11
• [Presentation] Enzymatic characterization of thiosulfate oxidation in epsilon-Proteobacteria isolated from deep-sea hydrothermal field (2007)
  • Author(s): 山本 正浩
  • Organizer: InterRidge Theoretical Institute
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2007-09-11
• [Remarks] JAMSTEC 研究成果データベース
• [Remarks]
  • URL: http://www.jamstec.go.jp/seika/jdb/index.do;jsessionid=7BA699C00FD3A42D6349691A4E3EED98