The cytotoxic mechanism and immunological role of CD4+CD8+ macrophages

• Project/Area Number: 19890081
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Kanazawa University
• Principal Investigator: BABA Tomohisa Kanazawa University, がん研究所, 助教 (00452095)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,075,000 (Direct Cost: ¥2,670,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,320,000 (Direct Cost: ¥1,320,000)
• Keywords: マクロファージ / 腫瘍免疫 / 細胞傷害活性 / CD4 / CD8 / 抗腫瘍効果 / granzyme / perforin / 腫瘍細胞 / RAET1

Research Abstract

ラット脾臓内CD4+CD8+マクロファージを用いて、数種類の腫瘍細胞に対する細胞傷害活性を検討し、またそのメカニズムを解析したところ、NK細胞と同様に、活性型NKレセプター(NKRP2)を介して腫瘍細胞表面上のRAET1分子を認識し、granzymeやperforinなどの細胞傷害因子を分泌することで、抗腫瘍細胞傷害活性を示すことが明らかとなった。

Research Products

• [Journal Article] Essential contribution of Ets-1 to constitutive Pim-3 expressi on in human pancreatic cancer cells. (2009)
  • Author(s): Li YY, Wu Y, Tsuneyama K, BabaT, Mukaida N.
  • Journal Title: Cancer science 100 Pages: 396-404
  • Peer Reviewed
• [Journal Article] CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process (2008)
  • Author(s): Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
  • Journal Title: J. Immunol 181 Pages: 6384-6394
  • Peer Reviewed
• [Journal Article] Rat CD4+CD8+ Macrophages Kill Tumor Cells through an NKG2D- and Granzyme/Perforin-Dependent (2008)
  • Author(s): Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A
  • Journal Title: Mechanism. J. Immunol 180 Pages: 2999-3006
  • Peer Reviewed
• [Journal Article] Tumor cell apoptosis induces tumor-specific immunity in a CC chemokine receptor 1- and 5-dependent manner in mice. (2008)
  • Author(s): Iida N, Nakamoto Y, Baba T, Kakinoki K, Li YY, Wu Y, Matsushima K, Kaneko S, Mukaida N.
  • Journal Title: Journal of Leukocyte Biology 84 Pages: 1001-1010
  • NAID: 120000997200
  • Peer Reviewed
• [Journal Article] CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung me tastasis process. (2008)
  • Author(s): Wu Y, Li YY, Matsushirma K, Baba T, Mukaida N.
  • Journal Title: Journal of Immunology 181 Pages: 6384-6394
  • Peer Reviewed
• [Journal Article] Rat CD4+CD8+ Macrophages Kill Tumor Cells through an NKG2D- and Granzyme/Perforin-Dependent Mechanism. (2008)
  • Author(s): Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A
  • Journal Title: Journal of Immunology 180 Pages: 2999-3006
  • Peer Reviewed
• [Journal Article] Protective Roles of the Fractalkine/CX3CL1-CX3CR1 Interactions in Alkali-Induced Corneal Neovascularization through Enhanced Antiangiogenic Factor Expression. (2008)
  • Author(s): Lu P, Li L, Kuno K, Wu Y, Baba T, Li YY, Zhang X, Mukaida N
  • Journal Title: Journal of Immunology 180 Pages: 4283-4291
  • Peer Reviewed
• [Presentation] CD4/CD8 double positive マクロファージの発見と自然免疫系における新しいエフェクター細胞としての展開 (2008)
  • Author(s): 馬場智久
  • Organizer: 日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] 血中抗原に対する免疫寛容誘導に関わるSirpa+胸腺樹状細胞の役割 (2008)
  • Author(s): 馬場智久, 呉俣, 向田直史
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
• [Presentation] CD4/CD8 double-positive monocytes/ macrophages : a subpopulation of the macrophage/dendritic cell lineage with a cytotoxic phenotype (2007)
  • Author(s): 馬場智久
  • Organizer: 16^<th> International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: 静岡
  • Year and Date: 2007-06-14
• [Presentation] CD4/CD8 double positiveマクロファージによる抗腫瘍メカニズムの解明 (2007)
  • Author(s): 馬場智久
  • Organizer: 日本病理学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-14
• [Presentation] CD4+/CD8+ マクロファージの抗腫瘍メカニズム (2007)
  • Author(s): Iwasaki S, Baba T, and lshizu A
  • Organizer: 日本免疫学会(poster presentation)
  • Place of Presentation: 東京
• [Presentation] CD4/CD8 double-positive monocytes/macrophages:a subpopulation of the macrophage/dendritic cedll lineage with a cytotoxic phenotype (2007)
  • Author(s): Baba T and Ishizu A
  • Organizer: 16th International Symposium on Molecular Cell Biology of Macrophages 2007 (poster presentation)
  • Place of Presentation: 静岡
• [Remarks]
  • URL: http://www.kanazawa-u.ac.jp/~ganken/bunsiseitai/JapContent.html
• [Remarks]
  • URL: http://www.kanazawa-u.ac.jp/~ganken/bunsiseitai/JapContent.html