Three-Dimensional Biodegradable Nanofibers for Multifunctional Cardiac Patch

Research Project

Project/Area Number	19F19362
Research Category	Grant-in-Aid for JSPS Fellows
Allocation Type	Single-year Grants
Section	外国
Review Section	Basic Section 21050:Electric and electronic materials-related
Research Institution	The University of Tokyo
Host Researcher	染谷隆夫東京大学, 大学院工学系研究科(工学部), 教授 (90292755)
Foreign Research Fellow	WANG CHUNYA 東京大学, 工学(系)研究科(研究院), 外国人特別研究員
Project Period (FY)	2019-11-08 – 2022-03-31
Project Status	Granted (Fiscal Year 2021)
Budget Amount *help	¥2,400,000 (Direct Cost: ¥2,400,000) Fiscal Year 2021: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 2020: ¥1,200,000 (Direct Cost: ¥1,200,000) Fiscal Year 2019: ¥700,000 (Direct Cost: ¥700,000)
Keywords	Nanomesh bioelectrodes / Silk / Electrospinning / Gold deposition
Outline of Research at the Start	1.Preparation of nanofiber membrane by electrospinningand fabrication　(2019) 2.Preparation of 3D electronic scaffold, culturing of cardiomyocytes, and assessment of assembly of cardiac tissues on the 3D electronic scaffold (2020) 3.3D Monitoring of in vitro electrophysiological activity of cultured cardiomyocytes, test of biocompatibility and biodegradability, and real-time 3D recording of electrophysiological signals of the as-prepared cardiac patch during long-term implantation (2021)
Outline of Annual Research Achievements	Biocompatible and biodegradable conductive biogels were designed and prepared with on-skin paintable capability for high-fidelity electrophysiological recordings　made from cost-effective, naturally-derived and food-exploitable constituents, and were easily synthesizable. As-designed biogels showed temperature-controlled reversible phase transition between viscous liquid and viscoelastic gel, which endowed them with on-skin painted capability, good biomechanical interaction with skin, as well as the ability as improved interfaces for both non-hairy and hairy skin for high-fidelity and long-term stable electrophysiological recording. Such on-skin paintable biogel offers a biocompatible, reliable, highly effective and stable interface for high-performance epidermal recording bioelectronics.
Current Status of Research Progress	Current Status of Research Progress 2: Research has progressed on the whole more than it was originally planned. Reason The concept about developing on-skin paintable biogel was first tried and demonstrated feasible, so the research went ahead as planned.
Strategy for Future Research Activity	To make the as-designed biogel suitable for on-skin usability, the phase transition temperature point of the biogel would be optimized by adjusting the crosslink density of biogel matrix. The physical, mechanical and electrical properties of the optimized biogel would be characterized to show its superior features compared to commercial conductive gels or pastes. ECG/EMG recording, and EEG recording through scalp with dense hairs would be conducted by applying such as-designed biogel as interface. The motion artifacts and long-term stability of electrophysiological recording based on such biogel would also be conducted to show its superiority.