Three-Dimensional Biodegradable Nanofibers for Multifunctional Cardiac Patch
| Project/Area Number |
19F19362
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 21050:Electric and electronic materials-related
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| Research Institution | The University of Tokyo |
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| Host Researcher |
染谷 隆夫 東京大学, 大学院工学系研究科(工学部), 教授 (90292755)
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| Foreign Research Fellow |
WANG CHUNYA 東京大学, 工学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Project Status |
Granted (Fiscal Year 2020)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2021: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2020: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2019: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Nanomesh bioelectrodes / Silk / Electrospinning / Gold deposition |
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| Outline of Research at the Start |
1.Preparation of nanofiber membrane by electrospinningand fabrication (2019)
2.Preparation of 3D electronic scaffold, culturing of cardiomyocytes, and assessment of assembly of cardiac tissues on the 3D electronic scaffold (2020)
3.3D Monitoring of in vitro electrophysiological activity of cultured cardiomyocytes, test of biocompatibility and biodegradability, and real-time 3D recording of electrophysiological signals of the as-prepared cardiac patch during long-term implantation (2021)
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| Outline of Annual Research Achievements |
According to the research plan for FY2019, nanomesh bioelectrodes have been tried to be prepared. First, silk fibroin which could be obtained by degumming natural silk cocoons has been tried as the biomaterial for electrospun nanofiber network, namely, nanomesh. Silk nanomesh was finally obtained by electrospinning method through optimizing spinning solution and parameters. It’s worth noting that degummed silk/CaCl2/formic acid solution was used as spinning solution to simplify the previously-reported process. To further prepare the nanomesh bioelectrode, gold was first tried to be deposited on silk nanomesh by thermal evaporation. However, the silk nanomesh became brittle and broken after gold deposition process, leading to the failure of preparing silk nanomesh bioelectrode.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
As a newcomer to the lab and this proposed research field, there was much need to learn besides designing and conducting experiments than I had originally planned. I was also met by more challenges than anticipated in fulfilling the experiments as planned. For example, the difficulty in obtaining the as-designed nanomesh bioelectrode which is composed of biomaterial nanomesh and biocompatible electronic materials is yet to be overcome. At present, because of the virus pandemic, the lab is closed preventing further experiments from being conducted to push forward the original plan.
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| Strategy for Future Research Activity |
First, try other biomaterials for the preparation of biocompatible nanomesh and try other biocompatible electronic materials and deposition methods for nanomesh bioelectrodes. Second, after successful preparation of nanomesh bioelectrodes, 3D nanofiber-based electronic scaffolds would be tried through stack-up configuration and step-by-step fabrication strategy. Then, the 3D nanofiber-based electronic scaffold would be utilized to culture cardiomyocytes through cooperation with other experienced labs.
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Report
(1 results)
Research Products
(1 results)
