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Non-destructive detection of chicken egg gender and viable embryos based on eggshell terahertz properties

Research Project

Project/Area Number 19F19396
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionKyoto University
Host Researcher 近藤 直  京都大学, 農学研究科, 教授 (20183353)
Foreign Research Fellow KHALIDUZZAMAN  京都大学, (連合)農学研究科(研究院), 外国人特別研究員
KHALIDUZZAMAN  京都大学, (連合)農学研究科(研究院), 外国人特別研究員
KHALIDUZZAMAN  京都大学, (連合)農学研究科(研究院), 外国人特別研究員
Project Period (FY) 2019-11-08 – 2022-03-31
Project Status Granted (Fiscal Year 2021)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2021: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥600,000 (Direct Cost: ¥600,000)
KeywordsEgg Shell / Teraheltz / Optical properties / Refractive index / Non-destructive / Precision poultry / 鶏卵 / テラヘルツ分光 / センシング / 雌雄判定
Outline of Research at the Start

卵殻の紫外から近赤外までの透過,反射,蛍光の分光情報を蓄積することにより,鶏卵中の品質,鮮度,鶏胚の非破壊モニタリングを行う。また,卵殻の厚さを非破壊計測可能な波長帯の探索,その消費速度の雌雄による違いを計測する。続いて鶏胚のバイタルサインのモニタリング可能なシステムを構築する。本実験成果により,孵化前の雌雄判定および孵化中の鶏胚の計測に貢献する。

Outline of Annual Research Achievements

We conducted several experiments on optical properties of eggshell in Terahertz Region (0.5-3.0 THz) to interpret biological phenomena of chicken eggshell and also conducted experiment on Hatching eggs toward predicting gender and hatching time based on eggshell optical properties. We found differences in image textural differences in SEM image, and in refractive index of eggshell from younger (refractive index = 2.45) and older (RI=2.65) parent flocks. The refractive index in terahertz region indicates the density of eggshell. Now, we could trace back mother birds` age based on eggshell characteristics. Based on the above observation, it is important to develop non-destructive protocol of refractive index measurement of eggs for industrial application. And we successfully developed a non-destructive protocol with correlation co-efficient (r=0.7).

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

We have observed and discovered many new biological phenomena in chicken eggshell and abled to develop protocol and interpret those biological phenomena using Terahertz signal of eggshell for the first time. For example, the biological age differences of eggshell can be traced using refractive index of eggshell in THz region (0.5-3.0 THz). And we developed a non-destructive refractive index estimation protocol for intact eggshell. We belief this protocol could extensively be used for precision poultry and egg industry in the future. Because refractive index of eggshell is not only important to interpret the biology but also have industrial significance for egg industry as it indicates the density and strength of the eggshell.
Additionally, we would like to mention here that we published several research papers in this fiscal years in reputed journals and attended conferences like ASABE and SPIE.

Strategy for Future Research Activity

In next fiscal year, we would like to focus mainly on analysis of experimental data which have been conducted recently. We conducted several experiments on optical properties of eggshell in Terahertz Region (0.5-3.0 THz) to interpret biological phenomena in Chicken and also conducted experiment on Hatching eggs toward predicting gender and hatching time. Additionally, we may conduct the following additional experiments as supplementary experiments. Thus, we may need to purchase some consumable items, necessary accessories and others.
Eggshell optical properties of various age and breed group of chickens using THz wave (reflectance and transmittance);
SEM images of eggshell (various age and breed group);

Besides, we may need some funds to publish scientific articles/reports and attending conferences and associated travel cost in next fiscal year.

Report

(2 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report

Research Products

(8 results)

All 2021 2020

All Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Estimation of refractive index of eggshell in intact egg using terahertz time-domain spectroscopy2021

    • Author(s)
      Khaliduzzaman, A., Shiraga, K., Kondo, N., and Ogawa, Y
    • Journal Title

      SPIE digital library

      Volume: 11789

    • DOI

      10.1117/12.2585199

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Chick Embryo Growth Modeling Using Near-Infrared Sensor and Non-Linear Least Square Fitting of Egg Opacity2020

    • Author(s)
      Khaliduzzaman, A.; Kashimori, A., Suzuki, T., Ogawa, Y., Kondo, N
    • Journal Title

      Sensors

      Volume: 20 (5888) Pages: 1-11

    • DOI

      10.3390/s20205888

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Non-destructive sex-specific monitoring of early embryonic growth rate in light brown broiler eggs using light transmission and its correlation with hatching time and chick weight. British Poultry Science2020

    • Author(s)
      Rahman, A., Syduzzaman, M., Khaliduzzaman, A., Fujitani, S., Kashimori, A., Suzuki, T., Ogawa, Y., Kondo, N.
    • Journal Title

      British Poultry Science

      Volume: 62 (1) Pages: 1-8

    • DOI

      10.1080/00071668.2020.1820952

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Association of fruit, pericarp, and epidermis traits with surface autofluorescence in green peppers2020

    • Author(s)
      Konagaya Keiji、Omwange Ken Abamba、Al Riza Dimas Firmanda、Khaliduzzaman Alin、Mart?nez Oliver Andrea、Rovira-M?s Francisco、Nagasato Hiroki、Ninomiya Kazunori、Kondo Naoshi
    • Journal Title

      Photochemical & Photobiological Sciences

      Volume: 19 Pages: 1630-1635

    • DOI

      10.1039/d0pp00236d

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Nondestructive Eggshell Thickness Measurement Technique Using Terahertz Waves.2020

    • Author(s)
      Alin Khaliduzzaman, Keiji Konagaya, Tetsuhito Suzuki, Ayuko Kashimori, Naoshi Kondo & Yuichi Ogawal.
    • Journal Title

      Scientfic Report

      Volume: 10 Pages: 1052-1052

    • DOI

      10.1038/s41598-020-57774-5

    • NAID

      120006888503

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Non-invasive Sensing of Egg Yolk Content to Detect Viable Chick Hatchlings2020

    • Author(s)
      Khaliduzzaman, A., Kashimori, A., Ogawa, Y., Suzuki, T., Kondo, N.
    • Organizer
      Annual International Meeting of American Society of Agricultural and Biological Engineering
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Estimation of refractive index of eggshell in intact egg using terahertz time-domain spectroscopy2020

    • Author(s)
      Khaliduzzaman, A., Shiraga, K., Kondo, N., and Ogawa, Y
    • Organizer
      Fourth international Seminar on Photonics, Optics and its Application
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gender Difference in UV-Induced Fluorescence Image of Non-incubated Chicken Eggs2020

    • Author(s)
      1.Khaliduzzaman, Yuichi Ogawa, Ayuko Kashimori, Tetsuhito Suzuki, Naoshi Kondo
    • Organizer
      Spring Meeting of Japan Poultry Science Association
    • Related Report
      2019 Annual Research Report

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Published: 2019-11-29   Modified: 2021-12-27  

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