Retracing the routes of invasion and evolutionary paths of the honey bee mite using genomic
| Project/Area Number |
19F19723
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 45020:Evolutionary biology-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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| Host Researcher |
ミケェエヴ アレクサンダー 沖縄科学技術大学院大学, 生態・進化学ユニット, 准教授 (90601162)
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| Foreign Research Fellow |
TECHER MAEVA 沖縄科学技術大学院大学, 生態・進化学ユニット, 外国人特別研究員
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| Project Period (FY) |
2019-07-24 – 2021-03-31
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| Project Status |
Granted (Fiscal Year 2020)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2020: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2019: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | parasites / mites / viruses / honey bees / coevolution |
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| Outline of Research at the Start |
As first of its kind, I plan to do population genomics of V. destructor at a global scale by sequencing the whole genome of 500 mites. The project is based on my previous results: i) the new reference V. destructor genome, ii) genomics pipeline used in Asian populations and iii) the so far largest Varroa mite world collection (< 800 samples from five continents) achieved in the proposed host Ecology and Evolution lab. I will be sequencing the whole genome of mites from native and invasive areas from all continents, different host populations and mite phenotypes.
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| Outline of Annual Research Achievements |
Western honey bee populations continuously fight against tenacious Asian enemies: ectoparasitic Varroa mites. The Western honey bee (A. mellifera) has been introduced into the native range of its sister species, the Eastern honey bee (A. cerana). This new, sudden contact between both species allowed parasite spillover. About 70 years ago, Varroa destructor, took this opportunity and successfully switched hosts from the Eastern to the Western honey bee. This parasite rapidly spread quasi-worldwide and became the main driver of honey bee colony losses. Concerns arise as ten years ago, an emerging threat, V. jacobsoni, also jumped onto the Western honey bee in Papua New Guinea.Despite a reported loss of genetic diversity and quasi-clonality in invasive populations, mites are persisting and evolving new traits such as acaricide resistance.
To help turning the tide in the Varroa-honey bees-beekeepers war, we recently developed new genomic resources to identify the mechanisms behind the mites' success. Here, I will present i) the first genomic insights into the demographic origins of V. destructor and V. jacobsoni jumps. Using mite species collected in their native ranges from both the ancestral and novel hosts, we were able to correctly reconstruct the temporal dynamics of the switch. We further found that hundreds of haplotypes were involved in the initial host switch, and, while greatly reduced, some gene flow between mites adapted to different hosts remains.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The samples were largely on hand, and given our previous experience with Varroa genomics, we were able to quickly make libraries for sequencing. In fact, we conducted preliminary investigations by sequencing a representative sub-sample of the total samples to ascertain the viability of this project. There results were surprising, and showed that we can, in fact, reconstruct Varroa biogeography. We presented these preliminary data at two international conferences.
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| Strategy for Future Research Activity |
The preliminary results indicate that we should be able to detect signals of migration and perhaps even local adaptation by Varroa. We will be finishing sequencing the rest of the samples in the first quarter of FY2020, and proceed to data analysis and writing up the major manuscript. The sequencing libraries have already been prepared and submitted to our sequencing center.
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Report
(1 results)
Research Products
(1 results)
