Establishment of enzymomics platform for discovery of disease-related protein functions
| Project/Area Number |
19H02846
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 37030:Chemical biology-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Komatsu Toru 東京大学, 大学院薬学系研究科(薬学部), 助教 (40599172)
|
|---|
| Project Period (FY) |
2019-04-01 – 2023-03-31
|
| Project Status |
Completed (Fiscal Year 2022)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
|
|---|
| Keywords | ケミカルバイオロジー / 創薬化学 |
|---|
| Outline of Research at the Start |
生体内には，数千種類を超える酵素が存在するが，いまだ疾患との関わりの理解が得られていない酵素は多く，これらは，未来の創薬標的，バイオマーカー候補としてその機能解明が待たれている．本研究では，酵素の活性を直接的に可視化することのできるケミカルバイオロジーの方法論に基づき，生体サンプル中の酵素活性を網羅的に評価する「enzymomics（enzymeのomics）」の技術開発をおこない，新たな創薬標的，バイオマーカー探索をおこなう技術基盤としてこれを確立することを目指す．
|
| Outline of Final Research Achievements |
In this project, we aimed to advance the concept of "enzymomics (omics of enzyme)" approach by giving it comprehensivity and sensitivity. Comprehensivity was realized by utilizing the concept of enzyme coupled assays, and the sensitivity was realized by introducing the single-molecule enzyme activity analysis platform and developing the probes suitable for them. We have accomplished them in the research period.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究を通じて，enzymomics の高度化に繋がる研究成果を，J. Am. Chem. Soc. 誌，Science Advances 誌，Cell Reports 誌などに発表し，分野の発展に努めた．特に，これを利用した疾患診断技術の重要性は世界的にも認知され，activity-based diagnosis という診断技術の意義が提唱されるに至っている．申請者らの研究は，その重要性の認知に先駆けて網羅性，感度を高めた形で実現しており，世界をリードする研究成果を継続的に発表できる基盤が整ったと言える．
|
Report
(4 results)
Research Products
(29 results)
-
-
[Journal Article] Discovery of an F-actin-binding small molecule serving as a fluorescent probe and a scaffold for functional probes2021
Author(s)
Takagi T, Ueno T, Ikawa K, Asanuma D, Nomura Y, Uno S, Komatsu T, Kamiya M, Hanaoka K, Okimura C, Iwadate Y, Hirose K, Nagano T, Sugimura K and Urano Y.
-
Journal Title
Science Advances
Volume: 7(47)
Issue: 47
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Covalent Self-labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings2020
Author(s)
Iwatate, J.R., Yoshinari, A., Yagi, N., Grzybowski, M., Ogasawara, H., Kamiya, M., Komatsu, T., Taki, M., Yamaguchi, S., Frommer, W.B. and Nakamura, M.
-
Journal Title
The Plant Cell
Volume: 32
Issue: 10
Pages: 3081-3094
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
[Journal Article] Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples2020
Author(s)
Shingo Sakamoto, Toru Komatsu, Rikiya Watanabe, Yi Zhang, Taiki Inoue, Mitsuyasu Kawaguchi, Hidehiko Nakagawa, Takaaki Ueno, Takuji Okusaka, Kazufumi Honda, Hiroyuki Noji, Yasuteru Urano
-
Journal Title
Science Advances
Volume: 6
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
