| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2022: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
For inducing in vitro oogenesis from human iPS cells via primordial germ cell-like cells (PGCLC), in this study, we attempted to establish the culture system that possible to induce human oogenesis in vitro. We tested and optimized the culture conditions and found that the primordial follicles can be induced from ooginia by culturing reconstituted ovaries for 15 weeks. In vitro induced primordial follicles exhibited the gene expression profiles similar with in vivo primordial follicles. Further improvement will enable us to induce in vitro oogenesis from human PGCLCs.
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