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Establishment of an early detection method by the environmental DNA of the alien diatom Cymbella janischii in Japan

Research Project

Project/Area Number 19K06187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionKyushu University

Principal Investigator

Kato-Unoki Yoko  九州大学, 工学研究院, 学術研究員 (10380560)

Co-Investigator(Kenkyū-buntansha) 栗原 暁  九州大学, 農学研究院, 助教 (00399817)
真山 茂樹  東京学芸大学, 教育学部, 名誉教授 (40199914)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords外来種対策 / ミズワタクチビルケイソウ / 環境DNA / 特異的検出 / 特許 / 外来種 / 外来珪藻 / 特異的検出系 / 河川水由来DNA / ジェノタイピング / 定量PCR / 正の相関 / Cymbella janischii / 内水面 / 環境保全 / Genetics / Single cell / 早期検出 / 保全
Outline of Research at the Start

ミズワタクチビルケイソウ (Cymbella janischii)は、近年日本に侵入した外来珪藻である。大量発生すると川底の石を覆い尽くすことから景観を損なうだけでなく、アユの漁場被害など生態系への影響が懸念されている。拡散を防止するには視覚的検出が困難な初期侵入時でも検出可能な系を確立して、分布河川を把握するとともに、持ち出さない・持ち込まない事が重要である。検出法としては高感度な環境DNA検査が有効と考えられるが、国内本種の遺伝子配列さえまだ調べられていない。そこで本研究では、国内本種の遺伝子配列を決定し、早期検出法を確立することを目的とした。

Outline of Final Research Achievements

We developed specific detection assays for an exotic diatom species, Cymbella janischii, from environmental DNA in Japan and applied it to a patent. These detection assays are capable of detecting Cymbella janischii from the benthic diatom on the stone as well as from river water with high sensitivity, and has been confirmed to correlate approximately with the actual amount of this species in rivers.
In the final year of the project, we made presentations at two international conferences and organized and held a workshop, "Riverine Anomalies - Problems and Latest Findings of an Alien Species, Cymbella janischii,” at the autumn meeting of the Japanese Society of Fisheries Science to introduced front-line research findings and deepened discussions on future required measures and research development.

Academic Significance and Societal Importance of the Research Achievements

河川内で本種の群生を目視で確認できる時には、既に大量に増殖している状態である。本検出法は侵入初期の早期発見とモニタリングに特に有効であり、河川利用者(漁協)や管理側での利活用に貢献することが期待される。さらに、今後河川水辺の国勢調査で環境DNA解析が本格的に取り入れられる場合には、国土全体での監視も可能になるものと期待される。
また、在不在の情報から、本種の生態、繁茂する河川の特性の解析などへの研究展開も可能になるものと期待される。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (28 results)

All 2024 2023 2022 2021 2019 Other

All Journal Article (12 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (2 results) Remarks (5 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] 懇話会ニュース 日本水産学会水産環境保全委員会2024

    • Author(s)
      鵜木陽子・清野聡子・栗林貴範・佐藤晋也・坪井潤一
    • Journal Title

      Nippon Suisan Gakkaishi

      Volume: 90 (2) Pages: 156-159

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of specific detection assays from environmental DNA of invasive diatom Cymbella janischii in Japan2024

    • Author(s)
      Yoko Kato-Unoki, Shigeki Mayama, Akira Kurihara, Toshifumi Minamoto
    • Journal Title

      Limnology

      Volume: 25 Issue: 3 Pages: 247-254

    • DOI

      10.1007/s10201-024-00751-y

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The nuisance invasive species Cymbella janischii in Japan: knowledge from studies and monitoring2023

    • Author(s)
      Yoko Kato-Unoki, Tamie Suzawa, Akira Kurihara, Yuta Takahashi, Toshifumi Minamoto, Yoshitsugu Masuda, Jun-ichi Tsuboi, Satoquo Seino, Shigeki Mayama
    • Journal Title

      The 26th International Diatom Symposium

      Volume: 1 Pages: 26-26

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution analysis of the invasive diatom Cymbella janischii using environmental DNA from benthic diatoms in river systems2023

    • Author(s)
      Yoko Kato-Unoki, Tamie Suzawa, Tran Thu Trang, Yoshitsugu Masuda, Jun-ichi Tsuboi, Satoquo Seino
    • Journal Title

      The eDNA Society International Meeting 2023

      Volume: 1 Pages: 104-104

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 研究会「河川の異変 -外来種ミズワタクチビルケイソウの問題と最新知見」の開催報告2023

    • Author(s)
      鵜木(加藤)陽子
    • Journal Title

      Diatom

      Volume: 39 Pages: 56-56

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic evidence for the invasion of Cymbella janischii (A. Schmidt) De Toni, 1891 in Japan2022

    • Author(s)
      Kato-Unoki Yoko、Kurihara Akira、Kuge Toshihiro、Shimasaki Yohei、Suzawa Yuzuru、Mayama Shigeki
    • Journal Title

      BioInvasions Records

      Volume: 11 Issue: 2 Pages: 409-415

    • DOI

      10.3391/bir.2022.11.2.14

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic evidence for the invasion of Cymbella janischii (A. Schmidt) De Toni, 1891 in Japan2022

    • Author(s)
      Kato-Unoki Y, Kurihara A, Kuge T, Shimasaki Y, Suzawa Y, Mayama S
    • Journal Title

      BioInvasions Records

      Volume: 11

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic evidence for the invasion of Cymbella janischii (A. Schmidt) De Toni in Japan2021

    • Author(s)
      Kato-Unoki Y, Kurihara A, Kuge T, Shimasaki Y, Suzawa Y, Mayama S
    • Journal Title

      Research Square

      Volume: -

    • DOI

      10.21203/rs.3.rs-767796/v1

    • Related Report
      2021 Research-status Report
    • Open Access
  • [Journal Article] A simple method for making transverse cleavages of diatom frustules for scanning electron microscopy and its application2021

    • Author(s)
      Nagisa Mayama, Shigeki Mayama
    • Journal Title

      Diatom Research

      Volume: 36 Issue: 4 Pages: 305-311

    • DOI

      10.1080/0269249x.2021.1986137

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 群馬県におけるミズワタクチビルケイソウの分布2019

    • Author(s)
      久下敏宏・山下耕憲・新井肇・栗原暁・鵜木(加藤)陽子
    • Journal Title

      群馬県水産試験場研究報告

      Volume: 25 Pages: 1-3

    • NAID

      40022018658

    • Related Report
      2019 Research-status Report
  • [Journal Article] 外来種ミズワタクチビルケイソウの出現の現状と環境DNAによる早期検出の試み2019

    • Author(s)
      洲澤謙・鵜木(加藤)陽子
    • Journal Title

      Diatom

      Volume: 35 Pages: 95-95

    • Related Report
      2019 Research-status Report
  • [Journal Article] 珪藻ー明日に架ける橋2019

    • Author(s)
      真山茂樹
    • Journal Title

      Diatom

      Volume: 35 Pages: 94-94

    • Related Report
      2019 Research-status Report
  • [Presentation] The nuisance invasive species Cymbella janischii in Japan: knowledge from studies and monitoring2023

    • Author(s)
      Yoko Kato-Unoki, Tamie Suzawa, Akira Kurihara, Yuta Takahashi, Toshifumi Minamoto, Yoshitsugu Masuda, Jun-ichi Tsuboi, Satoquo Seino, Shigeki Mayama
    • Organizer
      The 26th International Diatom Symposium
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distribution analysis of the invasive diatom Cymbella janischii using environmental DNA from benthic diatoms in river systems2023

    • Author(s)
      Yoko Kato-Unoki, Tamie Suzawa, Tran Thu Trang, Yoshitsugu Masuda, Jun-ichi Tsuboi, Satoquo Seino
    • Organizer
      The eDNA Society International Meeting 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 特異的検出法の開発とモニタリングから得た知見2023

    • Author(s)
      鵜木陽子
    • Organizer
      日本水産学会水産環境保全委員会研究会「河川の異変 -外来種ミズワタクチビルケイソウの問題と最新知見」
    • Related Report
      2023 Annual Research Report
  • [Presentation] はじめに2023

    • Author(s)
      真山茂樹
    • Organizer
      日本水産学会水産環境保全委員会研究会「河川の異変 -外来種ミズワタクチビルケイソウの問題と最新知見」
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 環境水からの外来種ミズワタクチビルケイソウの特異的検出2022

    • Author(s)
      鵜木(加藤)陽子, 真山茂樹, 栗原 暁, 清野聡子, 源 利文
    • Organizer
      環境DNA学会「あなたが主役のワークショップ」
    • Related Report
      2022 Research-status Report
  • [Presentation] 外来種ミズワタクチビルケイソウ検出法の開発2022

    • Author(s)
      鵜木(加藤)陽子(九大院農)・真山茂樹(東京学芸大教)・栗原 暁(九大院農)・清野聡子(九大院工)・源 利文(神戸大院発達)
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 外来種ミズワタクチビルケイソウの出現の現状と環境DNAによる早期検出の試み2019

    • Author(s)
      鵜木(加藤)陽子
    • Organizer
      日本珪藻学会公開シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Book] 外来魚に立ち向かう2024

    • Author(s)
      鵜木陽子・清野聡子 (p.23 「これってミズワタクチビルケイソウ? 診断の手順」を分担執筆)
    • Total Pages
      24
    • Publisher
      水産庁
    • Related Report
      2023 Annual Research Report
  • [Book] 河川の異変 : 外来種ミズワタクチビルケイソウの問題と最新知見2023

    • Author(s)
      鵜木陽子
    • Total Pages
      6
    • Publisher
      日本水産学会水産環境保全委員会
    • Related Report
      2023 Annual Research Report
  • [Remarks] 外来魚に立ち向かう

    • URL

      https://www.jfa.maff.go.jp/j/enoki/attach/pdf/naisuimeninfo-19.pdf

    • Related Report
      2023 Annual Research Report
  • [Remarks] ミズワタクチビルケイソウってどれのこと?

    • URL

      https://www.youtube.com/watch?v=qm9d4Ongk3I

    • Related Report
      2023 Annual Research Report
  • [Remarks] ミズワタクチビルケイソウが外来侵入種であることを証明

    • URL

      https://www.kyushu-u.ac.jp/ja/researches/view/770/

    • Related Report
      2022 Research-status Report
  • [Remarks] Researchgate

    • URL

      https://www.researchgate.net/profile/Yoko-Kato-Unoki/research

    • Related Report
      2021 Research-status Report
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/Unoki45/?lang=japanese

    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] ミズワタクチビルケイソウの特異的検出2023

    • Inventor(s)
      鵜木陽子
    • Industrial Property Rights Holder
      九州大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2023
    • Related Report
      2023 Annual Research Report
  • [Patent(Industrial Property Rights)] ミズワタクチビルケイソウの特異的検出2022

    • Inventor(s)
      鵜木陽子
    • Industrial Property Rights Holder
      九州大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-158624
    • Filing Date
      2022
    • Acquisition Date
      2022
    • Related Report
      2022 Research-status Report

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Published: 2019-04-18   Modified: 2025-01-30  

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