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Elucidation of molecular mechanism of water stress tolerance in rice internode.

Research Project

Project/Area Number 19K15815
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionNagoya University

Principal Investigator

Keisuke Nagai  名古屋大学, 生物機能開発利用研究センター, 助教 (30648473)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords洪水耐性 / 節間伸長 / イネ / 介在分裂組織 / ジベレリン / WOX遺伝子 / 浮イネ / 植物生理 / ジベレリン応答性
Outline of Research at the Start

洪水時に一般的な水田イネは酸素欠乏により溺死するのに対して、浮イネは急激な節間伸長により洪水時にも葉先を水面に出し、水面下の組織に効率的に酸素を供給することで洪水環境下においても生存することが可能である。本課題において、浮イネの保持する洪水耐性機構の理解を進め、イネの洪水耐性育種を目的とするとともに、得られた知見をコムギやトウモロコシなどのイネ科の主要畑作物へ応用することによる耐水性作物育種を目指す。

Outline of Final Research Achievements

Normal paddy rice drowns due to oxygen deprivation during flood. On the other hand, deepwater rice can survive in flooding environment because its internodes elongate rapidly, allowing the leaf tips to rise to the water surface and take up oxygen during flooding. Although it has been known that gibberellin, a plant hormone, is involved in elongation of deepwater rice, the detailed molecular mechanism has not yet been elucidated. In this study, I found two genes that regulate cell division in the internodes in response to gibberellin and elucidated the regulatory mechanism of internode elongation in rice by gibberellin.

Academic Significance and Societal Importance of the Research Achievements

イネ科作物の草丈は収量性と密接に関連しているため、重要な農業形質の一つであるが、これまでの草丈関連育種は耐倒伏性の観点から短稈化が主流であった。一方、東南アジアを中心に栽培されている浮イネは、洪水時に水位の上昇に合わせて茎(節間)を伸長させることで洪水による溺死を回避している。これまでに、浮イネの節間伸長には植物ホルモンの一種であるジベレリンが関与することが知られていたが、詳細な分子メカニズムは未解明であった。本研究は、ジベレリンによるイネの節間伸長制御機構を解明するとともに、近年、世界で多発する洪水によるイネ科作物の被害に対して、節間伸長による洪水耐性といった育種的応用を目指すものである。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (17 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Copenhagen(デンマーク)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] The University of Western Australia(オーストラリア)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Kobenhavns Universitet(デンマーク)

    • Related Report
      2019 Research-status Report
  • [Journal Article] SNORKEL Genes Relating to Flood Tolerance Were Pseudogenized in Normal Cultivated Rice2022

    • Author(s)
      Nagai Keisuke、Kurokawa Yusuke、Mori Yoshinao、Minami Anzu、Reuscher Stefan、Wu Jianzhong、Matsumoto Takashi、Ashikari Motoyuki
    • Journal Title

      Plants

      Volume: 11 Issue: 3 Pages: 376-376

    • DOI

      10.3390/plants11030376

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Noninvasive imaging of hollow structures and gas movement revealed the gas partial‐pressure‐gradient‐driven long‐distance gas movement in the aerenchyma along the leaf blade to submerged organs in rice2021

    • Author(s)
      Yin Yong‐Gen、Mori Yoshinao、Suzui Nobuo、Kurita Keisuke、Yamaguchi Mitsutaka、Miyoshi Yuta、Nagao Yuto、Ashikari Motoyuki、Nagai Keisuke、Kawachi Naoki
    • Journal Title

      New Phytologist

      Volume: 232 Issue: 5 Pages: 1974-1984

    • DOI

      10.1111/nph.17726

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolite and Phytohormone Profiling Illustrates Metabolic Reprogramming as an Escape Strategy of Deepwater Rice during Partially Submerged Stress2020

    • Author(s)
      Fukushima Atsushi、Kuroha Takeshi、Nagai Keisuke、Hattori Yoko、Kobayashi Makoto、Nishizawa Tomoko、Kojima Mikiko、Utsumi Yoshinori、Oikawa Akira、Seki Motoaki、Sakakibara Hitoshi、Saito Kazuki、Ashikari Motoyuki、Kusano Miyako
    • Journal Title

      Metabolites

      Volume: 10 Issue: 2 Pages: 68-68

    • DOI

      10.3390/metabo10020068

    • NAID

      120007132876

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Antagonistic regulation of the gibberellic acid response during stem growth in rice2020

    • Author(s)
      Nagai Keisuke et al.
    • Journal Title

      Nature

      Volume: 584 Issue: 7819 Pages: 109-114

    • DOI

      10.1038/s41586-020-2501-8

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of backcrossed pyramiding lines of the yield-related gene and the bacterial leaf blight resistant genes2020

    • Author(s)
      Shuto Yamada, Yusuke Kurokawa, Keisuke Nagai, Rosalyn B Angeles-Shim, Hideshi Yasui, Naruto Furuya, Atsushi Yoshimura, Kazuyuki Doi, Motoyuki Ashikari, and Hidehiko Sunohara
    • Journal Title

      Journal of international cooperation for agricultural development

      Volume: 18 Pages: 18-28

    • NAID

      120007182024

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diel O2 dynamics in partially and completely submerged deepwater rice: Leaf gas films enhance internodal O2 status, influence gene expression and accelerate stem elongation for ‘Snorkelling’ during submergence.2019

    • Author(s)
      Y. Mori, Y. Kurokawa, M. Koike. A. I. Malik, T. D. Colmer, M. Ashikari, O. Pedersen and K. Nagai
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 5 Pages: 973-985

    • DOI

      10.1093/pcp/pcz009

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 節間伸長促進因子SNORKEL-LIKEsの偽遺伝子化による水田イネ短稈化機構の解明2022

    • Author(s)
      永井啓祐、芦苅基行
    • Organizer
      日本育種学会第141回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネ節間伸長の分子機構の解明2022

    • Author(s)
      永井啓祐
    • Organizer
      日本育種学会第141回講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] The regulation of phase transition in rice internode2021

    • Author(s)
      永井啓祐、森欣順、石川慎、Rico Gamuyao、新美陽子、保浦徳典、福田萌莉、榊原均、古田智敬、久野 裕、佐 藤 和 広、赤木剛士、小嶋美紀子、竹林 裕美子、福島敦史、氷室泰代、小林正智、呉健忠、アキリ亘、吉田綾、辻寛之、佐藤豊、芦苅基行
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] イネ節間におけるジベレリンに応答した拮抗的伸長制御機構2021

    • Author(s)
      永井啓祐、森欣順、石川慎、Rico Gamuyao、新美陽子、保浦徳典、福田萌莉、榊原均、古田智敬、久野 裕、佐 藤 和 広、赤木剛士、小嶋美紀子、竹林 裕美子、福島敦史、氷室泰代、小林正智、呉健忠、アキリ亘、吉田綾、辻寛之、佐藤豊、芦苅基行
    • Organizer
      日本育種学会第139回講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] ACE1とDEC1によるイネ節間伸長のantagonistic制御22020

    • Author(s)
      永井啓祐、森欣順、石川慎、Rico Gamuyao、新美陽子、保浦徳典、福田萌莉、榊原均、古田智敬、久野 裕、佐 藤 和 広、赤木剛士、小嶋美紀子、竹林 裕美子、福島敦史、氷室泰代、小林正智、呉健忠、アキリ亘、吉田綾、辻寛之、佐藤豊、芦苅基行
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] イネ節間伸長の開始制御機構の解明2020

    • Author(s)
      永井啓祐、森欣順、石川慎、Rico Gamuyao、新美陽子、保浦徳典、福田萌莉、榊原均、古田智敬、久野 裕、佐 藤 和 広、赤木剛士、小嶋美紀子、竹林 裕美子、福島敦史、氷室泰代、小林正智、呉健忠、アキリ亘、吉田綾、辻寛之、佐藤豊、芦苅基行
    • Organizer
      日本育種学会第138回講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] イネ節間伸長における拮抗的制御機構2020

    • Author(s)
      永井啓祐,芦苅基行
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Antagonistic regulatory mechanism by accelerating and decelerating factors in internode elongation of rice for flooding adaptation.2019

    • Author(s)
      Keisuke Nagai, Shin Ishikawa, Mori Yoshinao, Yoko Niimi, Tokunori Hobo, Motoyuki Ashikari
    • Organizer
      The 13th International Society of Plant Anaerobiosis Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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