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The specification of meiotic recombination sites: chromosomal structures, genome and epigenome

Research Project

Project/Area Number 19K16045
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionNational Institute of Genetics

Principal Investigator

Imai Yukiko  国立遺伝学研究所, 遺伝形質研究系, 特任研究員 (00814782)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsゼブラフィッシュ / 減数分裂 / 相同組換え / 相同組み換え / 組換え
Outline of Research at the Start

減数分裂期の正常な染色体分配を担う組換えは、DNAの二重鎖切断(DSB)によって始まる。ヒトのDSB部位は配列特異的なDNA結合タンパクによるヒストン修飾で決まる一方で、組換えの起こりやすい領域には性差も見られる。従って、ゲノム・エピゲノム情報とは異なるレベルでのDSB制御が予想されるが、そのメカニズムは未知である。本研究では、新たな制御因子として染色体構造に着目した。ゼブラフィッシュをモデルとしてDSB部位のゲノム・エピゲノム情報と、DSB形成タンパクの局在ダイナミクスを解析し、「ゲノム・エピゲノム情報」と「染色体構造」が特定の領域にDSBを誘導する仕組みを明らかにする。

Outline of Final Research Achievements

In human and zebrafish males, meiotic recombination occurs preferentially near the ends of chromosomes. However, how recombination is induced in these regions remains largely unknown. In this project, mutant zebrafish lines deficient for meiotic chromosomal structures were examined for their phenotypes in meiotic recombination. In addition, genome-wide mapping of recombination initiation sites and a histone modification identified characteristic features of recombination sites.

Academic Significance and Societal Importance of the Research Achievements

減数分裂期に起こる相同組換えは、遺伝情報のシャッフリングによって生物に多様性をもたらす重要なメカニズムである。また、卵子や精子などの配偶子形成において正常な染色体分配を担うプロセスでもあることから、ヒトにおける不妊や染色体異常を理解する上で重要な課題である。
ヒトの精子形成では、染色体末端近傍で相同組換えが起こりやすいが、そのメカニズムは全くの未知であった。類似した特徴が見られるゼブラフィッシュをモデルとした本研究の成果は、組換え制御の新規メカニズムを理解するための足がかりになると期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Meiotic Chromosome Dynamics in Zebrafish2021

    • Author(s)
      Imai Yukiko、Olaya Ivan、Sakai Noriyoshi、Burgess Sean M.
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 9 Pages: 757445-757445

    • DOI

      10.3389/fcell.2021.757445

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sycp1 Is Not Required for Subtelomeric DNA Double-Strand Breaks but Is Required for Homologous Alignment in Zebrafish Spermatocytes2021

    • Author(s)
      Imai Yukiko、Saito Kenji、Takemoto Kazumasa、Velilla Fabien、Kawasaki Toshihiro、Ishiguro Kei-ichiro、Sakai Noriyoshi
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 9 Pages: 664377-664377

    • DOI

      10.3389/fcell.2021.664377

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes2020

    • Author(s)
      Takemoto Kazumasa*、Imai Yukiko*、Saito Kenji、Kawasaki Toshihiro、Carlton Peter M.、Ishiguro Kei-ichiro、Sakai Noriyoshi (*co-first authors)
    • Journal Title

      PLOS Genetics

      Volume: 16 Issue: 2 Pages: 1-1

    • DOI

      10.1371/journal.pgen.1008640

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Initiation of meiotic recombination in zebrafish2022

    • Author(s)
      今井裕紀子
    • Organizer
      遺伝研研究会「有性生殖における染色体・クロマチン・核動態に関する若手研究者の会」
    • Related Report
      2021 Annual Research Report
  • [Presentation] Sycp1 is not required for subtelomeric DNA double-strand breaks but is required for homologous alignment in zebrafish spermatocytes.2021

    • Author(s)
      Yukiko Imai
    • Organizer
      The Students and Postdocs Meiosis Workshop, v3.0, PDSM 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] The synaptonemal complex and recombination in zebrafish meiosis2021

    • Author(s)
      Yukiko IMAI and Noriyoshi SAKAI
    • Organizer
      第38回 染色体ワークショップ・第19 回 核ダイナミクス研究会
    • Related Report
      2020 Research-status Report
  • [Presentation] Sycp1 is not required for subtelomeric DNA double-strand breaks but is required for homologous alignment in zebrafish spermatocytes.2021

    • Author(s)
      Yukiko IMAI and Noriyoshi SAKAI
    • Organizer
      The Students and Postdocs Meiosis Workshop v3.0, PDSM 2021
    • Related Report
      2020 Research-status Report
  • [Presentation] Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes.2020

    • Author(s)
      Yukiko Imai and Noriyoshi Sakai
    • Organizer
      Meiosis In Quarantine
    • Related Report
      2020 Research-status Report
  • [Presentation] The synaptonemal complex and recombination in zebrafish meiosis2020

    • Author(s)
      Yukiko Imai
    • Organizer
      第43回日本分子生物学会年会・シンポジウム「Dynamic and structural regulation of chromosome inheritance in meiosis」
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes.2020

    • Author(s)
      Yukiko Imai
    • Organizer
      Meiosis In Quarantine(ビデオカンファレンス)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゼブラフィッシュをモデルとした減数分裂期相同組換えの開始メカニズムへのアプローチ2019

    • Author(s)
      今井裕紀子
    • Organizer
      第42回日本分子生物学会年会・フォーラム「有性生殖における染色体・クロマチン・核動態」
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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