Research on oral vaccine systems using lactic acid bacteria as vectors
| Project/Area Number |
19K16649
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 粘膜ワクチン / 乳酸菌 / DNAワクチン / 細菌様粒子 / Citrobacter rodentium / 経口ワクチン / 乳酸菌ベクター |
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| Outline of Research at the Start |
近年、全身性免疫（体内に侵入してきた病原体に有効な免疫）と粘膜免疫（病原体の体内への侵入を阻止する免疫）の両方を活性化できる「飲むワクチン」が注目されている。本研究は、安全かつ効率的に粘膜免疫を活性化できる組換え乳酸菌をベクターとして用いた飲むワクチン「乳酸菌ワクチン系」の開発を目指したもので、これまでの研究から明らかになってきた乳酸菌ワクチン系の課題克服を目的とした研究を行う。
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| Outline of Final Research Achievements |
In this study, we conducted basic research related to the development of a novel oral vaccine system using lactic acid bacteria (LAB) as a vaccine carrier. The oral (or nasal) immunogenicity of three different systems, including 1) vaccine antigen-expressing LAB, 2) antigen-bound bacterium-like particles (BLP) which is composed of the cell wall peptidoglycan structure of heat-killed LAB, 3) LAB carrying plasmid DNA that functions as a DNA vaccine, was evaluated in mice. No strong immunogenicity was observed by oral administration in any of the systems, but significant immunogenicity was observed by nasal administration. Among these, the nasal administration of antigen-bound BLP induces a strongest antigen-specific immune responses not only to the systemic but also to the respiratory and the intestinal mucosa.
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| Academic Significance and Societal Importance of the Research Achievements |
粘膜ワクチンは、粘膜組織を介して感染する病原体に対するワクチンとして理想的な系である。本研究は、乳酸菌を利用した安全かつ汎用性の高い粘膜ワクチン系の基盤技術の開発という学術的意義を持つ。本研究成果をさらに発展させ、多様な病原体に対する粘膜ワクチン開発に応用することで、様々な感染症対策に寄与できると考えられる。
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Report
(4 results)
Research Products
(18 results)
