Heart Cryopreservation using Antifreeze Glycoprotein in a Variable Magnetic Field
Project/Area Number |
19K18174
|
Research Category |
Grant-in-Aid for Early-Career Scientists
|
Allocation Type | Multi-year Fund |
Review Section |
Basic Section 55030:Cardiovascular surgery-related
|
Research Institution | Kanazawa University |
Principal Investigator |
|
Project Period (FY) |
2019-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | 不凍糖タンパク / 低温心保存法 / 再灌流実験 / 低温新保存 / 心保存 / 微弱電磁場 |
Outline of Research at the Start |
低温保存は長時間の臓器保存に有利であり,我々は,微弱電磁場による非凍結・過冷却状態環境による低温心保存の有用性を証明してきた.しかし,過冷却状態は非常に不安定であり実用化は困難であった.凝固点降下・氷結晶の成長抑制,低温環境下での細胞保護機能を有した生体物質である不凍糖タンパクは,過冷却状態を安定化させる効果が確認された.今回,「微弱電磁場」による心保存法と「微弱電磁場+不凍糖タンパク」による心保存法を比較し,低温心保存に不凍糖タンパクを用いる効果を動物実験にて証明する.
|
Outline of Final Research Achievements |
Surgically removed control rat hearts (n = 6) were immersed in UW solution while AFGP-treated hearts (AFGP group) (n = 6) were immersed in UW solution containing 500 μg/mL AFGP. After static hypothermic preservation, a Langendorff apparatus was used to reperfuse the coronary arteries. After 30, 60, 90, and 120 min the heart rate (HR), coronary flow (CF), cardiac contractile force (max dP/dt), and cardiac diastolic force (min dP/dt) were measured. Tissue water content (TWC) and tissue adenosine triphosphate (ATP) levels in the reperfused preserved hearts were also assessed. All parameters were compared between the control and AFGP groups.The higher HR, CF, max dP/dt, min dP/dt, and tissue ATP levels in the AFGP than control hearts suggested that AFGP conferred superior hemodynamic and metabolic functions. Thus, AFGP might be a useful additive for the static/nonfreezing hypothermic preservation of hearts.
|
Academic Significance and Societal Importance of the Research Achievements |
AFGPは単純浸漬・非凍結低温心保存の基質添加剤として有用である可能性が示された.臓器保存における、AFGPの有用性を示唆した報告は初めてである。 研究成果については、2019年の第119回日本外科学会定期学術集会にて発表し、2020年にPhysiological Researchにて出版された。
|
Report
(3 results)
Research Products
(1 results)