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Innovative method development to screen novel antimicrobial peptides by evolutionary genomics approach

Research Project

Project/Area Number 19K22294
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Shigenobu Shuji  基礎生物学研究所, 超階層生物学センター, 教授 (30399555)

Project Period (FY) 2019-06-28 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords抗菌ペプチド / RNA-seq / トランスクリプトーム解析 / 昆虫 / 共生 / 次世代シーケンシング
Outline of Research at the Start

抗菌ペプチドは、多細胞生物がもつ自然免疫で機能する生体防御物質で、侵略する微生物を殺すために用いられる。既存の抗生物質と比べて耐性を獲得しにくい特性があり近年注目されている。私は、昆虫アブラムシと共生細菌の共生機構解明の基礎研究に従事してきたが、その過程で偶然に、機能未知遺伝子の中に新規の抗菌ペプチド群を発見した。これらは従来の遺伝子予測プログラムでは予測が不可能であったが、一定の特徴を共有していることに気づいた。本研究では、そのような経験則をパイプラインとして実装し、昆虫=微生物共生系のゲノム・トランスクリプトームデータからシステマティックに新規抗菌ペプチドを探索する新規技術を開発する。

Outline of Final Research Achievements

Motivated by my earlier fortuitous discovery of novel antimicrobial peptides through a transcriptome analysis of bacteriocytes (insect cells specialized for symbiosis with endosymbionts), this study aimed to establish a pipeline for identifying new antimicrobial peptides, particularly within the framework of insect-microbe symbiotic systems. Our innovative method enabled the identification of a novel class of antimicrobial peptides derived from insects, including aphid BCR peptides. This research signifies a pivotal stride towards understanding and harnessing the potential of antimicrobial peptides from insect-microbe interactions.

Academic Significance and Societal Importance of the Research Achievements

抗菌ペプチドは、多細胞生物がもつ生体防御のための物質で、自然免疫反応によって、侵略してくる病原性微生物を攻撃するための分子である。既存の抗生物質と比べて耐性を獲得しにくいため、新規抗菌ペプチドの開発には大きな関心が寄せられている。本研究では、これまで注目されてなかった昆虫・微生物共生系に焦点を当て、新規抗菌ペプチドの探索パイプラインを開発し、その手法を用いた新規抗菌ペプチドの発見を成功させた。これらの新規抗菌ペプチドは、新規医薬品や農薬の開発に応用できる大きなポテンシャルを秘めているとともに、本研究で示したアプローチは、ゲノム時代の天然物創薬の新たな方向性を示している。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020 2019 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Complex host/symbiont integration of a multi-partner symbiotic system in the eusocial aphid Ceratovacuna japonica2022

    • Author(s)
      Yorimoto Shunta、Hattori Mitsuru、Kondo Maki、Shigenobu Shuji
    • Journal Title

      iScience

      Volume: 25 Issue: 12 Pages: 105478-105478

    • DOI

      10.1016/j.isci.2022.105478

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Massive expression of cysteine-containing proteins causes abnormal elongation of yeast cells by perturbing the proteasome2022

    • Author(s)
      Namba Shotaro、Kato Hisaaki、Shigenobu Shuji、Makino Takashi、Moriya Hisao
    • Journal Title

      G3 Genes|Genomes|Genetics

      Volume: - Issue: 6

    • DOI

      10.1093/g3journal/jkac106

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic and transcriptomic analyses of the subterranean termite <i>Reticulitermes speratus</i> : Gene duplication facilitates social evolution2022

    • Author(s)
      Shigenobu Shuji、Hayashi Yoshinobu、Watanabe Dai、Tokuda Gaku、Hojo Masaru Y. et al.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 3 Pages: 1-12

    • DOI

      10.1073/pnas.2110361119

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aphid hologenomics: current status and future challenges2022

    • Author(s)
      Shigenobu Shuji、Yorimoto Shunta
    • Journal Title

      Current Opinion in Insect Science

      Volume: 50 Pages: 100882-100882

    • DOI

      10.1016/j.cois.2022.100882

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antimicrobial Activities of Cysteine-rich Peptides Specific to Bacteriocytes of the Pea Aphid <i>Acyrthosiphon pisum</i>2019

    • Author(s)
      Uchi N, Fukudome M, Nozaki N, Suzuki M, Osuki K-I, Shigenobu S, Uchiumi T.
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 2 Pages: 155-160

    • DOI

      10.1264/jsme2.ME18148

    • NAID

      130007668480

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 昆虫ソシオゲノミクスの新展開 シロアリとアブラムシ2022

    • Author(s)
      重信秀治
    • Organizer
      イベリアトゲイモリゲノム解読シンポジウム& 第三回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Hologenomics of aphids: an integrative view of the insect and symbionts2021

    • Author(s)
      Shuji Shigenobu
    • Organizer
      The 93rd Annual Meeting of the Genetics Society of Japan
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] CRISPR/Cas9 によるエンドウヒゲナガアブラムシのゲノム編集2020

    • Author(s)
      重信秀治
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 共生ゲノミクス - 昆虫とバクテリアの細胞内共生から学ぶこと2020

    • Author(s)
      重信秀治
    • Organizer
      日本共生生物学会第4回大会(招待講演)
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Genome editing in the pea aphid2020

    • Author(s)
      Shuji Shigenobu
    • Organizer
      19th Annual Workshop at Bellairs - Emerging Model Systems
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] NGSが切り拓く昆虫研究のフロンティア2019

    • Author(s)
      重信秀治
    • Organizer
      九州大学昆虫科学・新産業創生研究センター設立記念 キックオフシンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Genomic Revelations of a Mutualism: Aphids and the Endosymbiont2019

    • Author(s)
      Shuji Shigenobu
    • Organizer
      The 2nd NIBB-Princeton Joint Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Ortholog Analyses Reveal Genomic Basis of Evolutionary Novelty - Lessons from Insect Genomes2019

    • Author(s)
      Shuji Shigenobu
    • Organizer
      The 67th NIBB Conference "Quest for Orthologs"
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Remarks] 基礎生物学研究所プレスリリース:真社会性をもつササコナフキツノアブラムシと2種類の共生細菌が...

    • URL

      https://www.nibb.ac.jp/press/2022/12/26.html

    • Related Report
      2022 Annual Research Report

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Published: 2019-07-04   Modified: 2024-01-30  

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