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Development of an analytical basis for elucidating the function of small spinule structures in the brain

Research Project

Project/Area Number 19K22469
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 46:Neuroscience and related fields
Research InstitutionUniversity of Fukui

Principal Investigator

Fukazawa Yugo  福井大学, 学術研究院医学系部門, 教授 (60343745)

Co-Investigator(Kenkyū-buntansha) 黒田 一樹  福井大学, 学術研究院医学系部門, 准教授 (60557966)
Project Period (FY) 2019-06-28 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
KeywordsSpinule / 電子顕微鏡 / spinule
Outline of Research at the Start

神経組織中の小棘構造 (spinule)や多胞体 (multivesicular body; MVB)の観察に最適化した電子顕微鏡解析法を構築しながら、特にspinuleを中心に海馬CA1領域と小脳皮質における種類と分布と出現頻度を明らかにするとともに、神経活動やシナプス可塑性との関係について遺伝子改変動物を用いた行動薬理学的手法と組み合わせて明らかにし、その基礎的な構造情報を取得する。微小膜構造の解析基盤を構築し神経機能における役割を同定することで、新規研究分野「細胞質移行による細胞間情報伝達の脳機能における役割とメカニズム」の基礎を構築する。

Outline of Final Research Achievements

The aim of this study is to develop the technical basis and analytical know-how to clarify the functional significance of small, infrequently observed subcellular structures in the brain, whose precise distribution and functional significance in the brain are still unknown. For this purpose, we developed an electron microscopic method optimized for the observation of spinules and multivesicular bodies (MVBs), and analyzed their localization and frequency in the brain under various conditions. As a result, we confirmed that quantitative analysis of low-frequency ultrastructures is possible by using a high-resolution scanning microscope equipped with a focused ion beam to obtain ultrastructural information over a wider area than conventional methods.

Academic Significance and Societal Importance of the Research Achievements

Spinule様構造は1962年に初めて報告されたが (Westrum & Blackstad)、多様性や機能的役割についてのその後の報告は限られ(Spacek & Harris 2004)、その制御メカニズムは未解明であり、従ってその生理的意義も解明されていない。今回の研究成果は、生理的意義の解明に向けた解析基盤を構築した点、また、同様な微小構造体の解析にも応用可能である点で神経科学上の学術的意義があり、今後様々な生体内微小構造体の分布解析を通して、その機能に迫る方法論を提供した点で神経科学研究に留まらず広く医学生物学研究の推進に貢献するものである。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report

Research Products

(8 results)

All 2021 2020 2019 Other

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Geometry and the Organizational Principle of Spine Synapses along a Dendrite2020

    • Author(s)
      Parajuli Laxmi Kumar、Urakubo Hidetoshi、Takahashi-Nakazato Ai、Ogelman Roberto、Iwasaki Hirohide、Koike Masato、Kwon Hyung-Bae、Ishii Shin、Oh Won Chan、Fukazawa Yugo、Okabe Shigeo
    • Journal Title

      eNeuro

      Volume: 7 Pages: 1-19

    • DOI

      10.1523/eneuro.0248-20.2020

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain.2020

    • Author(s)
      Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, Kawamura M, Fukazawa Y, Shigemoto R, Yamasaki M, Sakimura K, Watanabe M.
    • Journal Title

      Journal of Comparative Neurology

      Volume: 528 Issue: 6 Pages: 1003-1027

    • DOI

      10.1002/cne.24792

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions2020

    • Author(s)
      Eguchi Kohgaku、Velicky Philipp、Hollergschwandtner Elena、Itakura Makoto、Fukazawa Yugo、Danzl Johann Georg、Shigemoto Ryuichi
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 14 Pages: 63-63

    • DOI

      10.3389/fncel.2020.00063

    • NAID

      120006862680

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physical disuse contributes to widespread chronic mechanical hyperalgesia, tactile allodynia, and cold allodynia through neurogenic inflammation and spino-parabrachio-amygdaloid pathway activation.2020

    • Author(s)
      Ohmichi Y, Ohmichi M, Tashima R, Osuka K, Fukushige K, Kanikowska D, Fukazawa Y, Yawo H, Tsuda M, Naito M, Nakano T.
    • Journal Title

      PAIN

      Volume: - Pages: 1808-1823

    • DOI

      10.1097/j.pain.0000000000001867

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice2019

    • Author(s)
      Murata K, Kinoshita T, Fukazawa Y, Kobayashi Ke, Kobayashi Ka, Miyamichi K, Okuno H, Bito H, Sakurai Y, Yamaguchi M, Mori K, Manabe H.
    • Journal Title

      Scientific reports

      Volume: 9 Pages: 7132-7132

    • DOI

      10.1038/s41598-019-43580-1

    • NAID

      120006862679

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] FIB-SEM観察法によるシナプス結合における協調的前後構造の構築を支える分子メカニズムの解析2021

    • Author(s)
      深澤有吾、Elhanbaly Ruwaida、石川達也、村田航志、黒田一樹
    • Organizer
      第126回日本神経科学会全国学術集会・第98回日本生理学会大会 合同大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 歯を含む硬組織における感覚受容関連分子の発現と局在についての解析基盤の確立2019

    • Author(s)
      4.領家崇、黒田一樹、村田航志、酒井涼、吉村仁志、佐野和生、深澤有吾
    • Organizer
      日本解剖学会 第79回中部支部学術集会
    • Related Report
      2019 Research-status Report
  • [Remarks] 福井大学医学部脳形態機能学分野

    • URL

      https://www.med.u-fukui.ac.jp/laboratory/brain/

    • Related Report
      2019 Research-status Report

URL: 

Published: 2019-07-04   Modified: 2022-01-27  

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