Physiological role of primary cilium-derived extracellular vesicles in fine-tuning signal transduction in target cells.
| Project/Area Number |
19K23728
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
0701:Biology at molecular to cellular levels, and related fields
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
|
|---|
| Project Period (FY) |
2019-08-30 – 2022-03-31
|
| Project Status |
Granted (Fiscal Year 2020)
|
| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | Primary Cilium / Extracellular Vesicles / Signal Transduction / Cell Migration |
|---|
| Outline of Research at the Start |
The current knowledge of pcEVs functions is very limited. We hypothesize that pcEVs act as vessels carrying signaling molecules for regulating cellular responses. This work will help to answer questions about the roles of pcEVs by studying the cellular responses to pcEVs and cell entry mechanisms.
|
| Outline of Annual Research Achievements |
The aim of this study is to find the physiological role of primary cilium derived extracellular vesicles in acceptor cells. Proteomics analysis of the target cells after exposure to pcEVs was performed. The results showed the increase in proteins related to cell proliferation and metabolism in the target cells exposed to pcEVs which were in line with our previous findings of increased rate of migration in target cells after exposure to pcEVs. Furthermore, in order to know whether the above mentioned effects were truly from pcEVs, we optimized the method to purify pcEVs from the mixture of extracellular vesicles using exosome depletion and purification of pcEVs.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
the research project is going rather smoothly as we have already devised the method to purify pcEVs which is important for confirming the physiological effects of pcEVs in target cells.
|
| Strategy for Future Research Activity |
The next plan is to do the proteomics and RNA analysis of the purified pcEVs using our recently optimized purification methods and confirmation of the results through biochemical and biological assays.
|
Report
(2 results)
Research Products
(1 results)
