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Analysis on micro domain formation during xylem differentiation using in vitro differentiation system

Research Project

Project/Area Number 19K23760
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0702:Biology at cellular to organismal levels, and related fields
Research InstitutionNational Institute of Genetics

Principal Investigator

Fujita Satoshi  国立遺伝学研究所, 遺伝形質研究系, 博士研究員 (50844099)

Project Period (FY) 2019-08-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords植物 / 細胞壁 / リグニン / マイクロドメイン / 植物細胞 / 道管 / 植物細胞生物学 / リン酸化
Outline of Research at the Start

細胞壁に囲まれた植物の細胞においては特異的に細胞壁成分を変化させることによって特定の機能を持った細胞種をつくることが知られている。その時、細胞膜上に特定の因子を集合させた「マイクロドメイン」を細胞膜上につくることがある。これまでに植物においてはROPシグナルによるマイクロドメインの形成機構がいくつかの例で明らかにされてきたが実際にどのような因子・成分によって構成になっているのかは不明な点が多い。本研究では生化学的手法による網羅的解析や共焦点顕微鏡による因子の分布の時空間的解析を組み合わせて、マイクロドメインの全貌を明らかにし、制御機構のさらなる理解を目指す。

Outline of Final Research Achievements

In cells, numerous subcellular structures sustain cellular activities by their functions or shapes. In such contexts, the plasma membranes provide a vast area of 2D space for molecules to accumulate on the cell surfaces. Many proteins reside on the plasma membranes ubiquitously, but several specific molecules have been reported to establish microdomains on the plasma membranes. Although these microdomains attract many researchers, it is still unclear what kind of factors reside together in the specific microdomains. To approach this question, I planned to have a biochemical screen that allows us to maintain spatial information in the cells. More specifically, I focused on xylem cells which have clear microdomains on their cell surfaces. In this project, I focused on generating transgenic lines but still several issues to be overcome for comprehensive analysis.

Academic Significance and Societal Importance of the Research Achievements

細胞には、タンパク質だけでも数千、数万種類の分子が存在し、それぞれが適切な時に、適切な場所に配置されると考えられている。これまでに数え切れない種類の分子が蛍光タグを用いて可視化することで時空間的にどのような挙動をするかが調べられてきた。そこで本研究ではproximal labeling法を適用することにより、微小空間にどのようなタンパク質が局在をしているのかを網羅的に同定することを試みた。今回は、材料を確立することに終始したが、同時に本法の適用範囲に関して深く考える必要があることが示唆された。植物細胞に新技術を適用する試みとしては意義深いものであると考えている。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019

All Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (2 results)

  • [Journal Article] Two chemically distinct root lignin barriers control solute and water balance2021

    • Author(s)
      Reyt Guilhem、Ramakrishna Priya、Salas-Gonz?lez Isai、Fujita Satoshi、Love Ashley、Tiemessen David、Lapierre Catherine、Morreel Kris、Calvo-Polanco Monica、Flis Paulina、Geldner Niko、Boursiac Yann、Boerjan Wout、George Michael W.、Castrillo Gabriel、Salt David E.
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 2320-2320

    • DOI

      10.1038/s41467-021-22550-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-order mutants reveal an essential requirement for peroxidases but not laccases in Casparian strip lignification2020

    • Author(s)
      Rojas-Murcia Nelson、H?maty Kian、Lee Yuree、Emonet Aur?lia、Ursache Robertas、Fujita Satoshi、De Bellis Damien、Geldner Niko
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 46 Pages: 29166-29177

    • DOI

      10.1073/pnas.2012728117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SCHENGEN receptor module drives localized ROS production and lignification in plant roots2020

    • Author(s)
      Fujita Satoshi、De Bellis Damien、Edel Kai H、Koster Philipp、Andersen Tonni Grube、Schmid‐Siegert Emanuel、Denervaud Tendon Valerie、Pfister Alexandre、Marhavy Peter、Ursache Robertas、Doblas Veronica G、Barberon Marie、Daraspe Jean、Creff Audrey、Ingram Gwyneth、Kudla Jorg、Geldner Niko
    • Journal Title

      The EMBO Journal

      Volume: 39 Issue: 9

    • DOI

      10.15252/embj.2019103894

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A two-way molecular dialogue between embryo and endosperm is required for seed development2020

    • Author(s)
      Doll N. M.、Royek S.、Fujita S.、Okuda S.、Chamot S.、Stintzi A.、Widiez T.、Hothorn M.、Schaller A.、Geldner N.、Ingram G.
    • Journal Title

      Science

      Volume: 367 Issue: 6476 Pages: 431-435

    • DOI

      10.1126/science.aaz4131

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO22020

    • Author(s)
      Okuda Satohiro、Fujita Satoshi、Moretti Andrea、Hohmann Ulrich、Doblas Ver?nica G.、Ma Yan、Pfister Alexandre、Brandt Benjamin、Geldner Niko、Hothorn Michael
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 5 Pages: 2693-2703

    • DOI

      10.1073/pnas.1911553117

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 極性をもったSGN1の局在が細胞外空間でのROS産生領域を限定する2019

    • Author(s)
      藤田智史、Niko Geldner
    • Organizer
      第83回植物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 局所的なリグニン蓄積の分子機構2019

    • Author(s)
      藤田智史、Niko Geldner
    • Organizer
      細胞壁ネット研究会
    • Related Report
      2019 Research-status Report

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Published: 2019-09-03   Modified: 2022-01-27  

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