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Elucidation of the various mechanism of the pain mediated by GPR34

Research Project

Project/Area Number 19K24067
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0907:Oral science and related fields
Research InstitutionNagoya University

Principal Investigator

Sayo Akira  名古屋大学, 医学部附属病院, 医員 (50849445)

Project Period (FY) 2019-08-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords神経障害性疼痛 / GPR34 / ミクログリア / マクロファージ / 肥満細胞
Outline of Research at the Start

研究代表者は、脊髄後角のミクログリアに発現するG-protein coupled receptor 34(GPR34)を介したシグナルが、マウスにおいて神経障害性疼痛を増悪することを見いだした。しかしGPR34はミクログリアだけでなく、肥満細胞やCD169陽性マクロファージにも発現する。本研究では、GPR34を介した神経障害性疼痛増悪において、ミクログリア以外の経路の有無を評価するために、可能性のある経路をそれぞれ欠損させ、GPR34を介する疼痛発症のメカニズムの多様性の存在を明らかにする。

Outline of Final Research Achievements

The signaling pathways of GPR34 for neuropathic pain other than microglia were evaluated behaviorally and histologically in mice. Intrathecal administration of LysoPS, a ligand for GPR34, slightly reduced pain in both mast cell-deficient and macrophage-deficient mice. Histological evaluation of LysoPS before and after intrathecal administration showed that mast cells had different degrees of degranulation but were not significant. No clear difference was confirmed for macrophages before and after administration. GPR34-mediated pain may involve cells focused on this study, and it is suggested that more detailed analysis of these pain mechanisms may advance the elucidation of neuropathic pain.

Academic Significance and Societal Importance of the Research Achievements

神経障害性疼痛は持続的な病的疼痛であり、多くは慢性的な経過をたどる。この難治性疼痛の発症メカニズムは不明の点が多く、その解明により新たな治療法の開発が求められている。本研究は神経障害性疼痛の悪化に関与するGPR34のミクログリア以外の関与の可能性を示した。本研究が今後の治療法の開発や難治性の神経障害性疼痛の発生メカニズムの解明の一助になることが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (10 results)

All 2021 2020 2019

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Zonisamide ameliorates neuropathic pain partly by suppressing microglial activation in the spinal cord in a mouse model2020

    • Author(s)
      Koshimizu Hiroyuki、Ohkawara Bisei、Nakashima Hiroaki、Ota Kyotaro、Kanbara Shunsuke、Inoue Taro、Tomita Hiroyuki、Sayo Akira、Kiryu-Seo Sumiko、Konishi Hiroyuki、Ito Mikako、Masuda Akio、Ishiguro Naoki、Imagama Shiro、Kiyama Hiroshi、Ohno Kinji
    • Journal Title

      Life Sciences

      Volume: 263 Pages: 118577-118577

    • DOI

      10.1016/j.lfs.2020.118577

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] GPR34 in spinal microglia exacerbates neuropathic pain in mice2019

    • Author(s)
      Sayo Akira、Konishi Hiroyuki、Kobayashi Masaaki、Kano Kuniyuki、Kobayashi Hiroki、Hibi Hideharu、Aoki Junken、Kiyama Hiroshi
    • Journal Title

      Journal of Neuroinflammation

      Volume: 16 Issue: 1 Pages: 23-33

    • DOI

      10.1186/s12974-019-1458-8

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A dentigerous cyst associated with a supernumerary tooth (fourth molar) in the mandibular ramus: A case report2019

    • Author(s)
      Hara Kenji、Tsuchiya Shuhei、Hagiwara Sumitaka、Fujio Masahito、Sayo Akira、Hibi Hideharu
    • Journal Title

      Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology

      Volume: 31 Issue: 2 Pages: 98-102

    • DOI

      10.1016/j.ajoms.2018.08.005

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Comparison of biomechanical analysis after Le Fort I osteotomy with two square-shaped and four L-shaped titanium plates using 3D-FEA2021

    • Author(s)
      Sayo A, Fujio M, Tsuboi M, Sakai K, Okumura D, Hibi H
    • Organizer
      The 60th Congress of Korean Association of Maxillofacial Plastic and Reconstructive Surgeons
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 異なるプレートを用いたLe Fort I型骨切り術後の骨片の安定性に関する検討2020

    • Author(s)
      佐世 暁,藤尾正人,荻須宏太,坪井亮仁,酒井 陽, 日比英晴
    • Organizer
      第30回日本顎変形症学会総会・学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 広範囲顎骨支持型装置の周囲に下顎歯肉癌が再発した1例2020

    • Author(s)
      佐世 暁,岡部一登,日比英晴
    • Organizer
      第24回日本顎顔面インプラント学会総会・学術大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Le FortⅠ型骨切り術の固定法と術後の骨片変位量の検討2019

    • Author(s)
      藤尾正人、佐世暁、荻須宏太、土屋周平、酒井陽、日比英晴
    • Organizer
      第64回日本口腔外科学会総会 学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 急速に拡大して顔貌変形をきたした鼻口蓋管嚢胞の1例2019

    • Author(s)
      服部宇、坂口晃平、外山直人、佐世暁、日比英晴
    • Organizer
      第64回日本口腔外科学会総会 学術大会
    • Related Report
      2019 Research-status Report
  • [Presentation] インプラント体撤去窩に填入された骨補填材に感染が波及した1例2019

    • Author(s)
      佐世暁、岡部一登、日比英晴
    • Organizer
      第23回日本顎顔面インプラント学会総会 学術大会
    • Related Report
      2019 Research-status Report
  • [Book] Infected bone substitute after the bone augmentation in the maxilla: A case report.2021

    • Author(s)
      Kazuto Okabe, Akira Sayo, Yuya Ohta, Go Ohara, Noriyuki Yamamoto, Hideharu Hibi.
    • Total Pages
      54
    • Publisher
      Japanese Stomatological Society
    • ISBN
      9784990823733
    • Related Report
      2021 Annual Research Report

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Published: 2019-09-03   Modified: 2023-01-30  

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