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Fabrication of pre-collecting and collecting lymphatic vessels and identification of factors involved in lymphatic vessel formation

Research Project

Project/Area Number 19K24252
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0908:Society medicine, nursing, and related fields
Research InstitutionKanazawa University

Principal Investigator

Nakajima Yukari  金沢大学, 保健学系, 助教 (40846680)

Project Period (FY) 2019-08-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsリンパ管 / リンパ浮腫 / 再生医療 / 培養リンパ管 / 脂肪組織由来間葉系幹細胞
Outline of Research at the Start

リンパ浮腫には根治治療がなく、これを目指すためにはリンパ節郭清部のリンパ管再建が必要となる。よって、本研究ではリンパ管内皮細胞への分化能をもつ脂肪組織由来間葉系幹細胞からリンパ輸送能をもつ導出リンパ管、あるいは集合リンパ管を作製し、このリンパ管形成に関わる因子を同定する。これにより、平滑筋細胞を伴うリンパ管形成の分子機構を解明する。同定した因子や分子機構は、再生医療を用いたリンパ浮腫患者の根治治療やリンパ浮腫発症予防に役立ち、世界に先駆けるリンパ浮腫治療・予防法の開発に繋がることが期待される。

Outline of Final Research Achievements

In the fabrication of lymphatic vessel by three-dimensional culture, adipose tissue-derived mesenchymal stem cells were isolated from C57BL6/J mice and cultured in plates, and then capillary lymphatic vessel production was performed using the Layer-by-Layer method. However, the rate of cell death was increased using revised protocol. Thereby, we established the conditions for stable lymphatic vessel production by improving the cell adhesion rate to culture inserts, using fibroblasts as support cells, and studying the composition of the culture medium.
We are in the process of fabricating connecting lymphatic vessels, identifying factors involved in lymphatic vessel formation, and evaluating the three-dimensional structure of lymphatic vessels using the established protocol.

Academic Significance and Societal Importance of the Research Achievements

生体外で毛細リンパ管を作製し、これに関わる因子の同定を行うことにより、リンパ管形成因子やこれを促進する因子を同定することができる。これを元に、導出リンパ管、集合リンパ管と、よりリンパ輸送能の高いリンパ管を生体外で作製し、これらのリンパ管形成に関わる因子を同定することで、乳がんや子宮がんの手術時にリンパ節郭を受けた患者の郭清部において、リンパ管再建を行い、リンパ浮腫の発症予防や根治的治療を行うことが可能になる。本研究は、再生医療を用いた世界に先駆けるリンパ浮腫治療・予防法の開発に繋がることが期待される。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report

Research Products

(13 results)

All 2021 2020 2019 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Effects of inhibition of lymphangiogenesis by the vascular endothelial growth factor receptor 3 (VEGFR-3) inhibitor, MAZ51 on full thickness wounds in mice2021

    • Author(s)
      Takada Kana、Nakajima Yukari、Urai Tamae、Mukai Kanae、Asano Kimi、Okuwa Mayumi、Sugama Junko、Nakatani Toshio
    • Journal Title

      Veins and Lymphatics

      Volume: 10 Pages: 9385-9385

    • DOI

      10.4081/vl.2021.9385

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 破格,リンパ管小孔,リンパ浮腫,静脈注射,筋肉内注射,皮膚創傷治癒の研究:我々が行ってきた研究の概要2020

    • Author(s)
      中谷壽男,向井加奈恵,中島由加里
    • Journal Title

      Journal of Wellness and Health care

      Volume: 44 Pages: 1-10

    • NAID

      120006953890

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Novel Hyaluronate Lyase Involved in Pathogenicity of Streptococcus dysgalactiae subsp. equisimilis2020

    • Author(s)
      Nguyen VA, Ogura K, Matsue M, Takemoto N, Mukai K, Nakajima Y, Hoang TL, Iwata Y, Sakai N, Wada T, Hashimoto W, Okamoto S, Ichimura H.
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 552418-552418

    • DOI

      10.3389/fmicb.2020.552418

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pre-collecting lymphatic vessels form detours following obstruction of lymphatic flow and function as collecting lymphatic vessels2020

    • Author(s)
      Asano K, Nakajima Y, Mukai K, Urai T, Okuwa M, Sugama J, Konya C, Nakatani T
    • Journal Title

      PLOS ONE

      Volume: 15 Pages: 1-13

    • DOI

      10.1371/journal.pone.0227814

    • NAID

      120007006471

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Factors affecting superficial vein visibility at the upper limb in healthy young adults: A cross-sectional observational study2020

    • Author(s)
      Mukai K, Fujii T, Nakajima Y, Ishida A, Kato M, Takahashi M, Tsuda M, Hashiba N, Mori N, Yamanaka A, Nakatani T
    • Journal Title

      The Journal of Vascular Access

      Volume: in press Pages: 1-8

    • DOI

      10.1177/1129729820909187

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anatomically safe sites for intramuscular injections: a cross-sectional study on young adults and cadavers with a focus on the thigh2019

    • Author(s)
      Nakajima Yukari、Fujii Taiga、Mukai Kanae、Ishida Asami、Kato Moeka、Takahashi Mao、Tsuda Mihiro、Hashiba Nanami、Mori Namiko、Yamanaka Ayaka、Ozaki Noriyuki、Nakatani Toshio
    • Journal Title

      Human Vaccines & Immunotherapeutics

      Volume: 16 Pages: 189-196

    • DOI

      10.1080/21645515.2019.1646576

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Longitudinal investigation of <i>Propionibacterium acnes</i> burden and skin physiological function during menstruation in healthy women2019

    • Author(s)
      Mukai K, Ogai K, Kobayashi M, Ueda T, Nakajima Y, Nakatani T
    • Journal Title

      KEITAI KINOU (Structure and Function)

      Volume: 18 Issue: 1 Pages: 20-30

    • DOI

      10.11172/keitaikinou.18.20

    • NAID

      130007742837

    • ISSN
      1347-7145, 1884-6084
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of Indonesian and two types of Japanese honey on the acceleration of cutaneous wound healing: an experimental study in mice.2019

    • Author(s)
      Haryanto, Mukai K, Nakajima Y, Okuwa M, Kim C, Jiu, Sugama J, Nakatani T
    • Journal Title

      Journal of Health Technology Assessment in Midwifery

      Volume: 2 Pages: 80-88

    • NAID

      120007006476

    • Related Report
      2019 Research-status Report
  • [Journal Article] Topical estrogen application to wounds promotes delayed cutaneous wound healing in 80-week-old female mice.2019

    • Author(s)
      Mukai K, Nakajima Y, Asano K, Nakatani T
    • Journal Title

      PLoS One

      Volume: 14 Pages: 1-14

    • DOI

      10.1371/journal.pone.0225880

    • NAID

      120007006470

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Investigation of lymphedema resolution in mouse hindlimbs2020

    • Author(s)
      Nakajima Y, Asano K, Mukai K, Urai T, Okuwa M, Sugama J, Nakatani T
    • Organizer
      第40回日本看護科学学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 上肢の皮静脈が目視困難な条件の探索2019

    • Author(s)
      藤井太河,向井加奈恵,中島由加里,石田朝実,加藤萌香,高橋茉央,津田美宙,羽柴菜々美,森奈美子,山中郁佳,中谷壽男
    • Organizer
      コ・メディカル形態機能学会 第18回学術集会・総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 皮膚創傷治癒におけるリンパ管の関与2019

    • Author(s)
      浅野きみ,中島由加里,向井加奈恵,中谷壽男
    • Organizer
      第49回日本創傷治癒学会
    • Related Report
      2019 Research-status Report
  • [Remarks] 金沢大学医薬保健研究域保健学系 看護科学領域 臨床実践看護学講座 創傷看護技術学分野

    • URL

      https://wmn.w3.kanazawa-u.ac.jp/

    • Related Report
      2020 Annual Research Report

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Published: 2019-09-03   Modified: 2022-01-27  

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