| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Cell division failure causes a wide range of diseases, but the quantitative relationship between the magnitude of cell division defects and their effects on the body function is unknown. In this study, we newly developed a light-responsive mitotic inhibitor. By light control using this compound, various levels of cell division defects were induced with various temporal patterns in early zebrafish embryos. We found embryos were highly resistant to one or two rounds of whole-body cell division failures during 2-4 hours post-fertilization (hpf) and to more severe mitotic failures after 5 hpf with almost intact tissue formation. Especially in the latter case, we found that the spindle checkpoint plays an important role in reducing the lethality of mitotic abnormalities.
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