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Fluorescent imaging probes for epigenetic enzymes with specialized amino acids

Research Project

Project/Area Number 19KK0197
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 47:Pharmaceutical sciences and related fields
Research InstitutionNagoya City University

Principal Investigator

Nakagawa Hidehiko  名古屋市立大学, 医薬学総合研究院(薬学), 教授 (80281674)

Co-Investigator(Kenkyū-buntansha) 川口 充康  名古屋市立大学, 医薬学総合研究院(薬学), 准教授 (10735682)
家田 直弥  名古屋市立大学, 医薬学総合研究院(薬学), 講師 (00642026)
Project Period (FY) 2019-10-07 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords蛍光プローブ / エピジェネティクス / イメージング / ケミカルバイオロジー
Outline of Research at the Start

研究代表者はエピジェネティック制御酵素の1つSIRTが脱アシル化活性を触媒することに着目しSIRT活性検出蛍光プローブを開発し世界で初めて細胞内のSIRT活性をイメージングすることに成功した。一方、海外共同研究者の1人はペプチド・タンパク質合成に利用可能な特殊アミノ酸を開発しペプチドやタンパク質に任意の蛍光団を導入する技術を開発した。これらの成果を融合しエピジェネティック制御酵素(SIRT、HDAC等)の選択的ペプチド性及びタンパク質性プローブを開発する。プローブの性能をもう1人の海外共同研究者とともに解析し、高性能化することで多様なエピジェネティック制御のイメージング技術を開発する。

Outline of Final Research Achievements

We have developed a proprietary fluorescent probe for SIRT isozyme, a type of epigenetic enzyme. A peptide-based probe library was constructed by combining various peptide sequences and fluorescent quenchers. These probes showed different reactivity to each isozyme of SIRT, and one of them showed predominant reactivity to SIRT3. Chemical screening using probes that showed good reactivity to SIRT2 was also performed to identify inhibitors that effectively inhibit demyristoylation of SIRT2. By employing a cyclization strategy and partial D-isoform substitution, we found compounds that exhibit good SIRT2 selective enzyme inhibitory activity in cellular systems.

Academic Significance and Societal Importance of the Research Achievements

エピジェネティック制御は近年大きな注目を集めている遺伝子発現及び細胞制御機構の1つであり、生命維持の基本的機構に関わると共に、多くの疾患に関連することが明らかになりつつある。エピジェネティック制御酵素の活性検出蛍光プローブの開発は、選択的阻害剤など有用な研究ツール及び治療薬候補の開発に資する。本研究の成果は、生命機能の解明や疾患治療法の開発に貢献すると考えられる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (15 results)

All 2022 2021 2019 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (4 results) Remarks (3 results)

  • [Int'l Joint Research] University of Pennsylvania(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] University of Pennsylvania(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Development of Sirtuin Fluorescence Probes and Medicinal Chemistry Research Targeting SIRT Family2022

    • Author(s)
      Kawaguchi Mitsuyasu、Nakajima Yuya、Nakagawa Hidehiko
    • Journal Title

      Journal of Synthetic Organic Chemistry, Japan

      Volume: 80 Issue: 9 Pages: 831-842

    • DOI

      10.5059/yukigoseikyokaishi.80.831

    • ISSN
      0037-9980, 1883-6526
    • Year and Date
      2022-09-01
    • Related Report
      2022 Research-status Report
  • [Journal Article] Stereochemistries of mariannamides C and D, two lipohexapeptides, isolated from Mariannaea elegans NBRC1023012022

    • Author(s)
      Kan’ichiro Ishiuchi, Akiho Nagumo, Mitsuyasu Kawaguchi, Honoka Furuyashiki, Hidehiko Nakagawa, Dai Hirose
    • Journal Title

      Heterocycles

      Volume: 104 Issue: 10 Pages: 1822-1835

    • DOI

      10.3987/com-22-14728

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of Nucleoside Diphosphate-Bearing Fragile Histidine Triad-Imaging Fluorescence Probes with Well-Tuned Hydrophobicity for Intracellular Delivery2022

    • Author(s)
      Kawaguchi Mitsuyasu、Furuse Yuri、Ieda Naoya、Nakagawa Hidehiko
    • Journal Title

      ACS Sensors

      Volume: 7 Issue: 9 Pages: 2732-2742

    • DOI

      10.1021/acssensors.2c01273

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Set of Highly Sensitive Sirtuin Fluorescence Probes for Screening Small-Molecular Sirtuin Defatty-Acylase Inhibitors2021

    • Author(s)
      Yuya Nakajima, Mitsuyasu Kawaguchi, Naoya Ieda, Hidehiko Nakagawa
    • Journal Title

      ACS Medicinal Chemistry Letters

      Volume: 12 Issue: 4 Pages: 617-624

    • DOI

      10.1021/acsmedchemlett.1c00010

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe2021

    • Author(s)
      Mitsuyasu Kawaguchi, Hidehiko Nakagawa
    • Journal Title

      Methods in molecular biology

      Volume: 2274 Pages: 155-168

    • DOI

      10.1007/978-1-0716-1258-3_14

    • ISBN
      9781071612576, 9781071612583
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin2019

    • Author(s)
      Mitsuyasu Kawaguchi, Naoya Ieda, and Hidehiko Nakagawa
    • Journal Title

      J. Med. Chem.

      Volume: 62 Issue: 11 Pages: 5434-5452

    • DOI

      10.1021/acs.jmedchem.9b00315

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 細胞膜透過型ペプチド性 SIRT2 阻害剤の開発と細胞障害性評価2022

    • Author(s)
      古屋敷帆乃花、川口充康、家田直弥、中川秀彦
    • Organizer
      第68回日本薬学会東海支部総会・大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Sirtuin脱アシル化阻害剤のスクリーニングに適用可能なケミカルプローブ群の開発2021

    • Author(s)
      中嶋雄哉、川口充康、家田直弥、中川秀彦
    • Organizer
      第74回日本酸化ストレス学会 第21回日本NO学会合同学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] Sirtuin脱脂肪酸アシル化阻害剤開発を指向した蛍光プローブ群の開発2021

    • Author(s)
      中嶋雄哉、川口充康、家田直弥、中川秀彦
    • Organizer
      日本ケミカルバイオロジー学会 第15回年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Sirtuin脱ミリストイル化阻害剤のスクリーニングに適用可能なケミカルプローブ群の開発2021

    • Author(s)
      中嶋雄哉, 川口充康, 家田直弥, 中川秀彦
    • Organizer
      第67回日本薬学会東海支部大会
    • Related Report
      2021 Research-status Report
  • [Remarks] 名古屋市立大学 大学院薬学研究科 薬化学分野

    • URL

      http://www.phar.nagoya-cu.ac.jp/hp/ykg/Yakka/index.html

    • Related Report
      2023 Annual Research Report 2022 Research-status Report 2021 Research-status Report
  • [Remarks] 名古屋市立大学大学院薬学研究科薬化学分野

    • URL

      https://www.nagoya-cu.ac.jp/phar/grad/soyaku/iyaku/yakka.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 研究室Webサイト

    • URL

      https://www.nagoya-cu.ac.jp/phar/grad/soyaku/iyaku/yakka.html

    • Related Report
      2019 Research-status Report

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Published: 2019-10-10   Modified: 2025-01-30  

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