Comprehensive Platform for Studying Molecular Mechanisms Regulating Cardiomyocyte Maturation

• Project/Area Number: 19KK0219
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 53:Organ-based internal medicine and related fields
• Research Institution: Jichi Medical University
• Principal Investigator: 魚崎 英毅 自治医科大学, 医学部, 准教授 (90740803)
• Co-Investigator(Kenkyū-buntansha): 関 満 自治医科大学, 医学部, 講師 (20822357) ; 升本 英利 国立研究開発法人理化学研究所, 生命機能科学研究センター, 上級研究員 (70645754)
• Project Period (FY): 2019-10-07 – 2025-03-31
• Project Status: Granted (Fiscal Year 2023)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2022: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 心筋細胞 / 成熟 / iPS細胞 / 心臓発生 / 比較トランスクリプトーム / 心臓 / 発生・発達 / 多能性幹細胞

Outline of Research at the Start

多能性幹細胞から得られる心筋細胞（PSC-CMs）は再生医療に加えて、疾患研究や創薬への利用が期待されている。しかし、PSC-CMsは、成体の心筋細胞とはその特性に大きな乖離がある。今後の応用を進めるためには、ヒト心筋細胞の成熟過程の理解と、その理解に基づいた成熟法や解析基盤が必要となる。Johns Hopkins大学Chulan Kwon准教授は新生仔ラットの心臓へPSC-CMsを移植することで、成熟した成体型のPSC-CMsを得る生体内成熟法を確立した。本研究では、生体内成熟法を用いることで、成熟過程のトランスクリプトームと、成熟レポーター細胞の開発と検証を行う。

Outline of Annual Research Achievements

本研究では、心筋細胞の成熟メカニズムの解明に向けて、研究を進めている。
コロナ禍の状況が落ち着いた2023年度は研究開始以来初めてJohns Hopkins大学への訪問が実現し、今後の共同研究の進め方についての打ち合わせを行うことができた。Kwon研究室メンバー全員との打ち合わせを行ったが、研究開始当初予定していた心臓内移植法の技術を持つメンバーが学位取得に伴い研究室を離れること、またその技術継承がうまく行っておらず現時点ではiPS細胞由来心筋細胞の心臓内移植が困難であることが明らかとなった。そのため今後の方針として、胚盤胞補完法、子宮内移植法など新しい手法の開発も含めて検討を行うこととした。
さらに、魚崎Gではin vitroでの成熟法の研究を進めており、一定の成果が得られており、in vitro側からのアプローチについても進めている。

Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

iPS細胞由来心筋細胞を心臓内へと移植することで成熟させ、リファレンスデータを取る予定であったが、この実験を担当する予定であったKwon研究室メンバーが研究室を離れたことで、実験ができなくなっているため。

Strategy for Future Research Activity

新しい心筋細胞の移植法の開発、in vitroからのアプローチなどを進める予定である。

Research Products

• [Int'l Joint Research] Johns Hopkins University(米国)
• [Int'l Joint Research] Johns Hopkins University(米国)
• [Int'l Joint Research] Johns Hopkins University/NIH(米国)
• [Int'l Joint Research] Johns Hopkins大学/Max Planck Florida Institute(米国)
• [Int'l Joint Research] Johns Hopkins University(米国)
• [Int'l Joint Research] Max Planck Florida Institute(米国)
• [Journal Article] 心筋細胞の成熟制御メカニズムと再生医療への展開 (2024)
  • Author(s): 魚崎英毅, Nawin CHANTHRA
  • Journal Title: カレントテラピー Volume: 42 Pages: 39-44
• [Journal Article] An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis (2023)
  • Author(s): Hino Tomohiro、Omura Satoshi N.、Nakagawa Ryoya、Togashi Tomoki、Takeda Satoru N.、Hiramoto Takafumi、Tasaka Satoshi、Hirano Hisato、Tokuyama Takeshi、Uosaki Hideki、Ishiguro Soh、Kagieva Madina、Yamano Hiroyuki、Ozaki Yuki、Motooka Daisuke、Mori Hideto、Kirita Yuhei、Kise Yoshiaki et al.
  • Journal Title: Cell Volume: 186 Issue: 22 Pages: 4920-4935
  • DOI: 10.1016/j.cell.2023.08.031
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells (2023)
  • Author(s): Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki & Tsukasa Ohmori
  • Journal Title: COMMUNICATIONS MEDICINE Volume: 3 Issue: 1
  • DOI: 10.1038/s43856-023-00286-w
  • Peer Reviewed / Open Access
• [Journal Article] 疾患iPS細胞を用いたミトコンドリア病の病態・創薬研究 (2023)
  • Author(s): 徳山剛士, 魚崎英毅
  • Journal Title: 生体の科学 Volume: 74 Pages: 396-397
• [Journal Article] Cardiac progenitors instruct second heart field fate through Wnts (2023)
  • Author(s): Miyamoto Matthew、Kannan Suraj、Anderson Matthew J.、Liu Xihe、Suh David、Htet Myo、Li Biyi、Kakani Tejasvi、Murphy Sean、Tampakakis Emmanouil、Lewandoski Mark、Andersen Peter、Uosaki Hideki、Kwon Chulan
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 4
  • DOI: 10.1073/pnas.2217687120
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Total and reduced/oxidized forms of coenzyme Q10 in fibroblasts of patients with mitochondrial disease (2023)
  • Author(s): Watanabe, C., Osaka, H., Watanabe, M., Miyauchi, A., Jimbo, E. F., Tokuyama, T., Uosaki, H., Kishita, Y., Okazaki, Y., Onuki, T., Ebihara, T., Aizawa, K., Murayama, K., Ohtake, A., & Yamagata, T.
  • Journal Title: Mol Genet Metab Rep Volume: 34 Pages: 100951-100951
  • DOI: 10.1016/j.ymgmr.2022.100951
  • Peer Reviewed / Open Access
• [Journal Article] iPS細胞を用いたミトコンドリア病の病態解明 (2023)
  • Author(s): 1.徳山剛士, 魚崎英毅
  • Journal Title: 実験医学 Volume: 41 Pages: 720-726
• [Journal Article] Cross-Organ Transcriptomic Comparison Reveals Universal Factors During Maturation (2022)
  • Author(s): Kambhampati Sandeep、Murphy Sean、Uosaki Hideki、Kwon Chulan
  • Journal Title: Journal of Computational Biology Volume: 29 Issue: 9 Pages: 1031-1044
  • DOI: 10.1089/cmb.2021.0349
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Protective roles of MITOL against myocardial senescence and ischemic injury partly via Drp1 regulation (2022)
  • Author(s): Tokuyama T、Uosaki H、Sugiura A、Nishitai G、Takeda K、Nagashima S、Shiiba I、Ito N、Amo T、Mohri S、Nishimura A、Nishida M、Konno A、Hirai H、Ishido S、Yoshizawa T、Shindo T、Takada S、Kinugawa S、Inatome R、Yanagi S
  • Journal Title: iScience Volume: 25 Issue: 7 Pages: 104582-104582
  • DOI: 10.1016/j.isci.2022.104582
  • Peer Reviewed / Open Access
• [Journal Article] Prolonged Myocardial Regenerative Capacity in Neonatal Opossum (2022)
  • Author(s): Nishiyama Chihiro、Saito Yuichi、Sakaguchi Akane、Kaneko Mari、Kiyonari Hiroshi、Xu Yuqing、Arima Yuichiro、Uosaki Hideki、Kimura Wataru
  • Journal Title: Circulation Volume: 146 Issue: 2 Pages: 125-139
  • DOI: 10.1161/circulationaha.121.055269
  • Peer Reviewed
• [Journal Article] ミトコンドリア病のiPS細胞を用いた病態解明 (2022)
  • Author(s): 徳山剛士、安済達也、魚崎英毅
  • Journal Title: 小児内科 「症例から学ぶミトコンドリア病」 Volume: 54 Pages: 635-639
• [Journal Article] Sarcomere maturation: function acquisition, molecular mechanism, and interplay with other organelles (2022)
  • Author(s): Ahmed Razan E.、Tokuyama Takeshi、Anzai Tatsuya、Chanthra Nawin、Uosaki Hideki
  • Journal Title: Philosophical Transactions of the Royal Society B: Biological Sciences Volume: 377 Issue: 1864 Pages: 20210325-20210325
  • DOI: 10.1098/rstb.2021.0325
  • Peer Reviewed / Open Access
• [Journal Article] Sympathetic Neurons Regulate Cardiomyocyte Maturation in Culture (2022)
  • Author(s): Kowalski William J.、Garcia-Pak Iris H.、Li Wenling、Uosaki Hideki、Tampakakis Emmanouil、Zou Jizhong、Lin Yongshun、Patterson Kira、Kwon Chulan、Mukouyama Yoh-Suke
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 10
  • DOI: 10.3389/fcell.2022.850645
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cross-organ transcriptomic comparison reveals universal factors during maturation. (2022)
  • Author(s): Kambhampati S, Murphy S, Uosaki H, Kwon C
  • Journal Title: J Comput Biol Volume: －
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Systems for the Functional Evaluation of Human Heart Tissues Derived from Pluripotent Stem Cells (2022)
  • Author(s): Murata Kozue、Masumoto Hidetoshi
  • Journal Title: Stem Cells Volume: In press Issue: 6 Pages: 537-545
  • DOI: 10.1093/stmcls/sxac022
  • Peer Reviewed / Open Access
• [Journal Article] Noncanonical Notch signals have opposing roles during cardiac development (2021)
  • Author(s): Miyamoto Matthew、Andersen Peter、Sulistio Edrick、Liu Xihe、Murphy Sean、Kannan Suraj、Nam Lucy、Miyamoto William、Tampakakis Emmanouil、Hibino Narutoshi、Uosaki Hideki、Kwon Chulan
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 577 Pages: 12-16
  • DOI: 10.1016/j.bbrc.2021.08.094
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disease Modeling of Mitochondrial Cardiomyopathy Using Patient-Specific Induced Pluripotent Stem Cells (2021)
  • Author(s): Tokuyama Takeshi、Ahmed Razan Elfadil、Chanthra Nawin、Anzai Tatsuya、Uosaki Hideki
  • Journal Title: Biology Volume: 10 Issue: 10 Pages: 981-981
  • DOI: 10.3390/biology10100981
  • Peer Reviewed / Open Access
• [Journal Article] Rapid manipulation of mitochondrial morphology in a living cell with iCMM (2021)
  • Author(s): Miyamoto Takafumi、Uosaki Hideki、Mizunoe Yuhei、Han Song-Iee、Goto Satoi、Yamanaka Daisuke、Masuda Masato、Yoneyama Yosuke、Nakamura Hideki、Hattori Naoko、Takeuchi Yoshinori、Ohno Hiroshi、Sekiya Motohiro、Matsuzaka Takashi、Hakuno Fumihiko、Takahashi Shin-Ichiro、Yahagi Naoya、Ito Koichi、Shimano Hitoshi
  • Journal Title: Cell Reports Methods Volume: 1 Issue: 4 Pages: 100052-100052
  • DOI: 10.1016/j.crmeth.2021.100052
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Decreased Lamin B1 Levels Affect Gene Positioning and Expression in Postmitotic Neurons (2021)
  • Author(s): Noguchi Azumi、Ito Kenji、Uosaki Yuichi、Ideta-Otsuka Maky、Igarashi Katsuhide、Nakashima Hideyuki、Kakizaki Toshikazu、Kaneda Ruri、Uosaki Hideki、Yanagawa Yuchio、Nakashima Kinichi、Arakawa Hirokazu、Takizawa Takumi
  • Journal Title: Neuroscience Research Volume: 173 Pages: 22-33
  • DOI: 10.1016/j.neures.2021.05.011
  • Peer Reviewed / Open Access
• [Journal Article] PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2 (2021)
  • Author(s): Murphy Sean A.、Miyamoto Matthew、Kervadec Anais、Kannan Suraj、Tampakakis Emmanouil、Kambhampati Sandeep、Lin Brian Leei、Paek Sam、Andersen Peter、Lee Dong-Ik、Zhu Renjun、An Steven S.、Kass David A.、Uosaki Hideki、Colas Alexandre R.、Kwon Chulan
  • Journal Title: Nature Communications Volume: 12 Issue: 1
  • DOI: 10.1038/s41467-021-21957-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9. (2021)
  • Author(s): Byambaa S, Uosaki H, Ohmori T, Hara H, Endo H, Nureki O, Hanazono Y.
  • Journal Title: Mol Ther Methods Clin Dev Volume: 20 Pages: 451-462
  • DOI: 10.1016/j.omtm.2021.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins. (2021)
  • Author(s): Ahmed Razan E.、Chanthra Nawin、Anzai Tatsuya、Koiwai Keiichiro、Murakami Tomoki、Suzuki Hiroaki、Hanazono Yutaka、Uosaki Hideki
  • Journal Title: Journal of Visualized Experiments Volume: 169 Issue: 169
  • DOI: 10.3791/62129
  • Peer Reviewed
• [Journal Article] Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells (2021)
  • Author(s): Abe Tomoyuki、Uosaki Hideki、Shibata Hiroaki、Hara Hiromasa、Sarentonglaga Borjigin、Nagao Yoshikazu、Hanazono Yutaka
  • Journal Title: Experimental Hematology Volume: 95 Pages: 46-57
  • DOI: 10.1016/j.exphem.2020.12.006
  • Peer Reviewed / Open Access
• [Journal Article] Novel device prototyping for endoscopic cell sheet transplantation using a three-dimensional printed simulator (2020)
  • Author(s): Osada Hiroaki、Ho Wen-Jin、Yamashita Hideki、Yamazaki Kazuhiro、Ikeda Tadashi、Minatoya Kenji、Masumoto Hidetoshi
  • Journal Title: Regenerative Therapy Volume: 15 Pages: 258-264
  • DOI: 10.1016/j.reth.2020.10.007
  • NAID: 120006898124
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function (2020)
  • Author(s): Abulaiti Mosha、Yalikun Yaxiaer、Murata Kozue、Sato Asako、Sami Mustafa M.、Sasaki Yuko、Fujiwara Yasue、Minatoya Kenji、Shiba Yuji、Tanaka Yo、Masumoto Hidetoshi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 19201-19201
  • DOI: 10.1038/s41598-020-76062-w
  • Peer Reviewed / Open Access
• [Journal Article] Strategies for immune regulation in iPS cell-based cardiac regenerative medicine (2020)
  • Author(s): Murata Kozue、Ikegawa Masaya、Minatoya Kenji、Masumoto Hidetoshi
  • Journal Title: Inflammation and Regeneration Volume: 40 Issue: 1 Pages: 36-36
  • DOI: 10.1186/s41232-020-00145-4
  • Peer Reviewed / Open Access
• [Journal Article] Accuracy of spiked cell counting methods for designing a pre-clinical tumorigenicity study model (2020)
  • Author(s): Osada H, Kawatou M, Takeda M, Jo JI, Murakami T, Tabata Y, Minatoya K, Yamashita JK, Masumoto H.
  • Journal Title: Heliyon Volume: 6 Issue: 7 Pages: 311-318
  • DOI: 10.1016/j.heliyon.2020.e04423
  • Peer Reviewed / Open Access
• [Journal Article] A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure (2020)
  • Author(s): Nishio Hiroomi、Minatoya Kenji、Masumoto Hidetoshi
  • Journal Title: Journal of Visualized Experiments Volume: 159 Issue: 159
  • DOI: 10.3791/60931
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair (2020)
  • Author(s): Nakane Takeichiro、Abulaiti Mosha、Sasaki Yuko、Kowalski William J.、Keller Bradley B.、Masumoto Hidetoshi
  • Journal Title: Journal of Visualized Experiments Volume: 160 Issue: 160 Pages: 258-264
  • DOI: 10.3791/61246
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Generation of novel <i>Il2rg</i>-knockout mice with clustered regularly interspaced short palindromic repeats (CRISPR) and Cas9 (2020)
  • Author(s): BYAMBAA Suvd、UOSAKI Hideki、HARA Hiromasa、NAGAO Yasumitsu、ABE Tomoyuki、SHIBATA Hiroaki、NUREKI Osamu、OHMORI Tsukasa、HANAZONO Yutaka
  • Journal Title: Experimental Animals Volume: 69 Issue: 2 Pages: 189-198
  • DOI: 10.1538/expanim.19-0120
  • NAID: 130007835079
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] A Novel Fluorescent Reporter System Identifies Laminin-511/521 as Potent Regulators of Cardiomyocyte Maturation (2020)
  • Author(s): Chanthra Nawin、Abe Tomoyuki、Miyamoto Matthew、Sekiguchi Kiyotoshi、Kwon Chulan、Hanazono Yutaka、Uosaki Hideki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 4249-4249
  • DOI: 10.1038/s41598-020-61163-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Brief Review of Current Maturation Methods for Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes (2020)
  • Author(s): Ahmed Razan Elfadil、Anzai Tatsuya、Chanthra Nawin、Uosaki Hideki
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 8
  • DOI: 10.3389/fcell.2020.00178
  • Peer Reviewed / Open Access
• [Journal Article] Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice (2020)
  • Author(s): Nishizono Hirofumi、Darwish Mohamed、Uosaki Hideki、Masuyama Nanami、Seki Motoaki、Abe Hiroyuki、Yachie Nozomu、Yasuda Ryohei
  • Journal Title: Journal of Visualized Experiments Volume: － Issue: 158 Pages: 1-1
  • DOI: 10.3791/60808
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Comparative Transcriptome Landscape of Mouse and Human Hearts (2020)
  • Author(s): Tatsuya Anzai, Takanori Yamagata, Hideki Uosaki
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 8
  • DOI: 10.3389/fcell.2020.00268
  • Peer Reviewed / Open Access
• [Presentation] Where are We with the Gene Therapy for Cardiomyopathy? (2024)
  • Author(s): Hideki Uosaki
  • Organizer: MIS-Korea 2024
  • Int'l Joint Research / Invited
• [Presentation] Gene Therapy for Mybpc3 Mutant Cardiomyopathy Models (2024)
  • Author(s): 魚崎英毅, 安済達也, 川上亮, 徳山剛士, AHMED Razan, TORIZAL F Gandhi, 石井秀樹, 小板橋紀通
  • Organizer: 第88回日本循環器学会学術集会
• [Presentation] 多能性幹細胞由来心筋細胞の成熟化と疾患モデルへの挑戦 (2024)
  • Author(s): 魚崎英毅
  • Organizer: 第101回日本生理学会大会
  • Invited
• [Presentation] Maturation Culture Exaggerated Disease Phenotype in Mitochondrial Cardiomyopathy iPSC-derived Cardiomyocytes (2023)
  • Author(s): Razan E Ahmed, Nawin Chanthra, Kumiko A Iwabuchi, Takeshi Tokuyama, Hideki Uosaki
  • Organizer: Weinstein Conference
  • Int'l Joint Research
• [Presentation] Combinations of Nuclear Receptor Activations Revealed Disease Phenotype in iPSC-CMs Derived from Patients with Mitochondrial Cardiomyopathy. (2023)
  • Author(s): Razan E Ahmed, Nawin Chanthra, Kumiko A Iwabuchi, Takeshi Tokuyama, Hideki Uosaki
  • Organizer: Cardiovascular Bioenginnering (CVBE) Symposium 2023
  • Int'l Joint Research
• [Presentation] Cardiomyopathy Disease Models with Matured iPSC-derived Cardiomyocytes. (2023)
  • Author(s): Hideki Uosaki
  • Organizer: Cardiovascular Bioenginnering (CVBE) Symposium 2023
  • Int'l Joint Research / Invited
• [Presentation] ヒト肥大型心筋症遺伝子変異ノックインマウスにおける遺伝子治療による病的な心臓リモデリングの改善 (2023)
  • Author(s): 小板橋紀通, 川上亮, 徳山剛士, 安済達也, Ahmed Ranzan, Torizal Fuad Gandhi, 長尾恭光, 魚崎英毅, 石井秀樹
  • Organizer: 第71 回日本心臓病学会学術集会
• [Presentation] 肥大型心筋症モデルマウスに対する遺伝子治療と病的リモデリングの抑制 (2023)
  • Author(s): 魚崎英毅, 安済達也, 川上亮, 徳山剛士, AHMED Razan, TORIZAL F Gandhi, 長尾恭光, 石井秀樹, 小板橋紀通
  • Organizer: 第29回日本遺伝子細胞治療学会学術集会
• [Presentation] ES/iPS細胞由来心筋細胞の成熟化とその生理学的特性 (2023)
  • Author(s): Nawin Chanthra, 魚崎英毅
  • Organizer: 生理研研究会
  • Invited
• [Presentation] Gene Therapy Improves Pathological Cardiac Remodeling in Human Hypertrophic Cardiomyopathy Gene Variant Knock-in Mice (2023)
  • Author(s): Norimichi Koitabashi, Ryo Kawakami, Takeshi Tokuyama, Razan E Ahmed, Fuad Gandhi Torizal, Tatsuya Anzai, Yasumitsu Nagao, Hideki Ishii, Hideki Uosaki
  • Organizer: American Heart Association
  • Int'l Joint Research
• [Presentation] 疾患iPS細胞を用いたBOLA3ミトコンドリア心筋症の病態と新規形態異常Mito-Ballの解明 (2023)
  • Author(s): 徳山剛士、魚崎英毅
  • Organizer: 第22回日本ミトコンドリア学会年会
• [Presentation] 心筋細胞の成熟を司る転写ネットワーク (2023)
  • Author(s): 魚崎英毅
  • Organizer: 第46回日本分子生物学会年会
  • Invited
• [Presentation] ミトコンドリア機能から見た心筋細胞と皮膚 (2023)
  • Author(s): 魚崎 英毅
  • Organizer: 第11回ケラチノサイト・免疫と皮膚を勉強する会
  • Invited
• [Presentation] Development of new strain of immunodeficient mice for pig-to-mouse xenotransplantation (2023)
  • Author(s): Munkh-Erdene Natsagdorj, Hiromasa Hara, Suvd Byambaa, Fumio Nakahara, Yasumitsu Nagao, Hideki Uosaki, Yutaka Hanazono
  • Organizer: 第25回異種移植研究会
• [Presentation] Cardiomyopathy mouse models for the development of gene therapy (2023)
  • Author(s): Hideki Uosaki
  • Organizer: MIS-KOREA
  • Int'l Joint Research / Invited
• [Presentation] Hormone and Nuclear Receptor Activations Enhance Cardiomyocyte Maturation and Improve Fidelity of Using PSC-CMs as a Mitochondrial Disease Model (2023)
  • Author(s): Nawin Chanthra, Hideki Uosaki
  • Organizer: 第87回日本循環器学会学術集会
• [Presentation] Hormone and nuclear receptor activations are required for cardiomyocyte maturation (2022)
  • Author(s): "Nawin Chanthra, Razan E. Ahmed, Tatsuya Anzai, Takeshi Tokuyama, Kumiko A. Iwabuchi, Yutaka Hanazono, Hideki Uosaki"
  • Organizer: "XXIV World Congress International Society for Heart Research"
  • Int'l Joint Research
• [Presentation] Targeted genome repair in selectively expanded mouse long-term hematopoietic stem cells (2022)
  • Author(s): Suvd Byambaa, Hideki Uosaki, Hiromasa Hara, Munkh-Erdene Natsagdorj, Tsukasa Ohmori, Satoshi Yamazaki, Yutaka Hanazono
  • Organizer: 第28回日本遺伝子細胞治療学会学術集会
• [Presentation] マウスES細胞由来心筋細胞とアデノ随伴ウイルスを用いた拘束型心筋症の疾患モデリング (2022)
  • Author(s): 安済達也、魚崎英毅、Chanthra Nawin、森田裕介、福井沙織、古井貞浩、鈴木峻、岡健介、横溝亜希子、松原大輔、松本歩、佐藤智幸、関満、山形崇倫
  • Organizer: 第58回日本小児循環器学会
• [Presentation] ゲノム編集を用いた先天性疾患モデル動物の作出と解析 (2022)
  • Author(s): 魚崎 英毅
  • Organizer: 第62回日本先天異常学会
  • Invited
• [Presentation] 心筋症研究のためのiPS細胞モデル (2022)
  • Author(s): 魚崎 英毅
  • Organizer: 第70回 日本心臓病学会
  • Invited
• [Presentation] Development of a New Immunodeficient Mouse Strain Capable of Pig Cell Engraftment (2022)
  • Author(s): Munkh-Erdene Natsagdorj, Hiromasa Hara, Suvd Byambaa, Fumio Nakahara, Yasumitsu Nagao, Hideki Uosaki, Yutaka Hanazono
  • Organizer: 第8回日本先進医工学ブタ研究会研究集会
• [Presentation] ミトコンドリア酵素MITOLの低下は心臓老化や心筋梗塞を増悪し、MITOLの活性化は心筋梗塞の重症化を抑制する (2022)
  • Author(s): 徳山剛士、魚崎英毅、柳茂
  • Organizer: 第45回日本分子生物学会年会
• [Presentation] Disease Modeling of Mitochondrial Cardiomyopathy Using Patient-Derived Induced Pluripotent Stem Cells (2022)
  • Author(s): Razan E Ahmed, Nawin Chanthra, Kumiko A Iwabuchi, Takeshi Tokuyama, Hideki Uosaki
  • Organizer: ISHR/CVMW
• [Presentation] 心筋細胞の成熟メカニズム解明に向けた挑戦 (2022)
  • Author(s): 魚崎 英毅
  • Organizer: 第21回日本心臓血管発生研究会
  • Invited
• [Presentation] 循環器基礎研究の呼び声 (2022)
  • Author(s): 魚崎 英毅
  • Organizer: 第263回日本循環器学会関東甲信越地方会
  • Invited
• [Presentation] Recapturing Cardiomyopathy with Induced Pluripotent Stem Cells (2022)
  • Author(s): Hideki Uosaki
  • Organizer: MIS-KOREA
  • Int'l Joint Research / Invited
• [Presentation] Nuclear Receptors Coordinately Regulate Cardiomyocyte Maturation (2022)
  • Author(s): Nawin Chanthra, Razan E. Ahmed, Tatsuya Anzai, Takeshi Tokuyama, Yutaka Hanazono, Hideki Uosaki
  • Organizer: 第86回日本循環器学会学術集会
  • Int'l Joint Research
• [Presentation] 重症心不全治療の現状と展望-多能性幹細胞への期待 (2022)
  • Author(s): 升本英利
  • Organizer: 第86回日本循環器学会学術集会
  • Invited
• [Presentation] 心血管系細胞多層体移植による心臓再生医療 (2022)
  • Author(s): 升本英利
  • Organizer: 第21回日本再生医療学会総会
  • Invited
• [Presentation] Organ-on-a-Chip を応用した心臓組織機能評価 (2022)
  • Author(s): 升本英利
  • Organizer: 第21回日本再生医療学会総会
  • Invited
• [Presentation] Human iPS cell-derived heart organoids and the medical application. (2022)
  • Author(s): Masumoto H
  • Organizer: Frontiers in Stem Cell & Organoid Medicine Symposium 2022 (CuSTOM-RIKEN joint symposium 2022)
  • Int'l Joint Research / Invited
• [Presentation] Implication of Hormonal and Mechanical Regulation in Maturation of Cardiomyocytes Derived from Pluripotent Stem Cells (2021)
  • Author(s): Hideki Uosaki, Razan Elfadil Ahmed, Nawin Chanthra
  • Organizer: 第5回 JCS-Council Forum on Basic CardioVascular Research
  • Invited
• [Presentation] 疾患iPS細胞によるミトコンドリア心筋症研究 (2021)
  • Author(s): 魚崎 英毅
  • Organizer: 第20回日本ミトコンドリア学会年会
  • Invited
• [Presentation] 比較トランスクリプトームと幹細胞モデルから見る心筋細胞の成熟制御機構 (2021)
  • Author(s): 魚崎 英毅 他
  • Organizer: 生理研心血管研究会2021
  • Invited
• [Presentation] Effect of Hormones and Nuclear Receptor Agonists on Maturation of Cardiomyocytes derived from Pluripotent Stem Cells (2021)
  • Author(s): Chanthra N, Ahmed RE, Anzai T, Tokuyama T, Iwabuchi KA, Hanazono Y, Uosaki H
  • Organizer: ISSCR/JSRM 2021 Tokyo International Symposium.
  • Int'l Joint Research
• [Presentation] Sarcomere shortening of pluripotent stem cell-derived cardiomyocytes using fluorescent-tagged sarcomere proteins. (2021)
  • Author(s): Ahmed RE, Chanthra N, Anzai T, Koiwai K, Murakami T, Suzuki H, Hanazono Y, Uosaki H
  • Organizer: ISSCR/JSRM 2021 Tokyo International Symposium.
  • Int'l Joint Research
• [Presentation] Cardiovascular regenerative medicine supported by tissue engineering. (2021)
  • Author(s): Masumoto H.
  • Organizer: ISSCR/JSRM 2021 Tokyo International Symposium
  • Int'l Joint Research
• [Presentation] iPS細胞由来心筋細胞を用いた疾患研究の基盤構築 (2021)
  • Author(s): 魚崎 英毅
  • Organizer: CVMW2020 心血管代謝週間
  • Invited
• [Presentation] 疾患iPS細胞を用いた病態解明に向けた基盤構築 (2021)
  • Author(s): 魚崎 英毅
  • Organizer: 第20回日本再生医療学会総会
  • Invited
• [Presentation] 蛍光標識サルコメアを利用したiPS細胞由来心筋細胞のサルコメアショートニング (2021)
  • Author(s): 魚崎英毅, 安済達也, Nawin Chanthra, Razan E Ahmed, 花園 豊
  • Organizer: 第20回再生医療学会
• [Presentation] Disease Modeling of Cardiomyopathy Using Pluripotent Stem Cells. (2021)
  • Author(s): Hideki Uosaki
  • Organizer: MIS-KOREA
  • Int'l Joint Research / Invited
• [Presentation] iPS細胞を用いた心筋症モデリング (2021)
  • Author(s): 魚崎英毅
  • Organizer: 第 10 回 Cardiovasucular Thrombosis Seminar
  • Invited
• [Presentation] Research for Cardiac Regenerative Medicine and Heart Diseases Using Pigs as a Large Mammal. (2021)
  • Author(s): Masumoto H.
  • Organizer: 第85回日本循環器学会学術集会
  • Invited
• [Presentation] Identifying Cross-Organ Similarities During Development and Maturation Arrest of Pluripotent Stem Cell Differentiation (2020)
  • Author(s): Sandeep Kambhampati, Sean Murphy, Hideki Uosaki, Chulan Kwon
  • Organizer: BMES 2020 Virtual Annual Meeting
  • Int'l Joint Research
• [Presentation] In vitro maturation of cardiomyocytes derived from stem cells. (2020)
  • Author(s): Hideki Uosaki
  • Organizer: MIS-KOREA
  • Int'l Joint Research / Invited
• [Presentation] Heart Failure Treatment Using Engineered 3D Cardiac Sheets. (2020)
  • Author(s): Masumoto H.
  • Organizer: 第84回日本循環器学会学術集会
  • Invited
• [Presentation] ヒトiPS細胞由来心血管系細胞多層体を用いた新たな重症心不全治療法 (2020)
  • Author(s): 升本 英利
  • Organizer: 第19回日本再生医療学会総会（共催学術セミナー2）
  • Invited
• [Presentation] ヒトiPS細胞由来心血管系細胞多層体を用いた心不全治療法の開発 (2020)
  • Author(s): 升本 英利
  • Organizer: 第24回日本心不全学会学術集会
  • Invited
• [Presentation] Cardiovascular Regenerative Medicine Supported By Biomaterials And Drug Delivery System. (2020)
  • Author(s): Masumoto H.
  • Organizer: Controlled Release Society Virtual Annual Meeting 2020
  • Int'l Joint Research / Invited
• [Presentation] Hideki Uosaki, Nawin Chanthra, Yutaka Hanazono (2020)
  • Author(s): Hideki Uosaki, Nawin Chanthra, Yutaka Hanazono
  • Organizer: 第8回IRG Meeting
• [Presentation] In vitro maturation of cardiomyocytes derived from stem cells. (2020)
  • Author(s): Hideki Uosaki
  • Organizer: MIS-Korea 2020
  • Int'l Joint Research / Invited
• [Presentation] PHENOTYPICAL CORRECTION OF X-SCID MICE AFTER CRISPR/CAS9-MEDIATED UNIVERSAL GENOME-EDITING THERAPY OF HEMATOPOIETIC STEM CELLS (2019)
  • Author(s): Suvd Byambaa, Hideki Uosaki, Hiromasa Hara, Hiroaki Shibata, Tomoyuki Abe,Yasumitsu Nagao, Osamu Nureki, Tsukasa Ohmori, Yutaka Hanazono
  • Organizer: Frontiers in Genome Engineering 2019
  • Int'l Joint Research
• [Presentation] ESTABLISHMENT OF SIMULTANEOUS TRIPLE REPORTER SYSTEM FOR HDR/NHEJ/DSB (2019)
  • Author(s): Hiromasa Hara, Hideki Uosaki, Tomoyuki Abe, Hiroaki Shibata, Osamu Nureki, Yutaka Hanazono
  • Organizer: Frontiers in Genome Engineering 2019
  • Int'l Joint Research
• [Presentation] FEASIBILITY STUDY OF HOMOLOGOUS RECOMBINATION-BASED GENOME-EDITING THERAPY IN A SWINE MODEL OF X-SCID (2019)
  • Author(s): Yutaka Hanazono, Hiromasa Hara, Hideki Uosaki, Osamu Nureki
  • Organizer: Frontiers in Genome Engineering 2019
  • Int'l Joint Research / Invited
• [Presentation] 相同組換え修復を利用したHPRT1遺伝子のノックイン効率向上 (2019)
  • Author(s): 樋口真希、小澤高嶺、河野宏光、魚崎英毅、花園豊、太田邦史
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Transcriptional Network Regulating Cardiomyocyte Maturation (2019)
  • Author(s): Hideki Uosaki
  • Organizer: 第42回日本分子生物学会年会
  • Invited
• [Presentation] Visualizations of Cardiomyocyte Maturation on Extracellular Matrices Using a Novel Myom2-RFP Reporter Line (2019)
  • Author(s): Nawin Chanthra, Yutaka Hanazono, Hideki Uosaki
  • Organizer: CVMW 2019
• [Book] In. Haider KH(ed). Stem Cells: Latest Advances (2021)
  • Author(s): Chanthra N, Uosaki H
  • Total Pages: 255
  • Publisher: Springer Nature
• [Book] 「疾患モデルの作成と利用－循環器疾患2021」（堀内久徳、柳田素子、猪原匡史、冨本秀和、並河徹 編） (2021)
  • Author(s): 西園 啓文、魚崎 英毅
  • Total Pages: 594
  • Publisher: エル・アイ・シー
• [Book] In: Yoshinori Yoshida (ed). Pluripotent Stem-Cell Derived Cardiomyocytes (Methods in Molecular Biology) (2021)
  • Author(s): Anzai T, Hara H, Nawin C, Sadahiro T, Ieda M, Hanazono Y, Uosaki H
  • Total Pages: 304
  • Publisher: Springer Nature/Humana
• [Book] In: Yoshinori Yoshida (ed). Pluripotent Stem-Cell Derived Cardiomyocytes (Methods in Molecular Biology) (2021)
  • Author(s): Masumoto H.
  • Total Pages: 304
  • Publisher: Springer Nature/Humana
• [Patent(Industrial Property Rights)] 心筋細胞の成熟方法、心筋細胞の成熟度評価方法（以下省略） (2020)
  • Inventor(s): 魚崎英毅
  • Industrial Property Rights Holder: 魚崎英毅
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Overseas
• [Patent(Industrial Property Rights)] 細胞外マトリックス、成熟心筋細胞製造方法、創薬支援方法、治療方法、成熟心筋細胞、及び心筋細胞成熟キット (2020)
  • Inventor(s): 魚崎英毅、関口清俊
  • Industrial Property Rights Holder: 自治医科大学、大阪大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2020-017809
  • Filing Date: 2020
• [Patent(Industrial Property Rights)] 心筋細胞の成熟方法、心筋細胞の成熟度評価方法、成熟心筋細胞の純化方法、創薬支援方法、心疾患の治療方法、成熟心筋細胞マーカー、レポーター細胞、成熟心筋細胞、心筋細胞成熟度評価装置 （以下省略） (2019)
  • Inventor(s): 魚崎 英毅
  • Industrial Property Rights Holder: 自治医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-218998
  • Filing Date: 2019