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Niche-based cross-talk among muscle satellite, motor neuron and end plate in sensing mechanical stimuli during contraction of skeletal muscle cells

Research Project

Project/Area Number 19KK0254
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
Research InstitutionToyohashi Sozo University

Principal Investigator

Goto Katsumasa  豊橋創造大学, 保健医療学部, 教授 (70239961)

Co-Investigator(Kenkyū-buntansha) 江川 達郎  京都大学, 人間・環境学研究科, 准教授 (00722331)
横山 真吾  豊橋創造大学, 保健医療学部, 講師 (30706859)
Project Period (FY) 2019-10-07 – 2025-03-31
Project Status Completed (Fiscal Year 2024)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2023: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2022: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsサルコペニア / niche / 筋衛星細胞 / 血管内皮増殖因子
Outline of Research at the Start

本研究では、筋衛星細胞と運動神経細胞・神経筋接合部のニッチに着目して、運動神経細胞を介した収縮情報のネットワークを中心とした骨格筋の可塑性発現機構を明らかにし、サルコペニア克服策確立のための知的基盤を形成することを目的とする。本研究の目的達成には、共焦点顕微鏡と画像解析ソフトウエアを用いた筋衛星細胞と運動神経細胞・神経筋接合部の空間的位配置の3次元(3D)解析と3D定量化技術が重要な位置を占める。そこで、骨格筋組織の透明化技術、共焦点顕微鏡・画像解析ソフトウエアを用いた卓越した解析技術を確立したミネソタ大学のAsakura博士との国際共同研究として実施する。

Outline of Final Research Achievements

In an international collaboration with the University of Minnesota, USA, this study investigated the molecular mechanisms underlying the impact of physical exercise on skeletal muscle cells. Specifically, the study examined the morphology of the neuromuscular junction and vascular endothelial growth factor (VEGF) in these cells. The research period was extended by one year due to the COVID-19. This study demonstrated the important role of the neuromuscular junction's structure as a sensing mechanism for motor stimuli. The study also confirmed that VEGF is expressed in skeletal muscle cells and stimulates their growth.

Academic Significance and Societal Importance of the Research Achievements

健康長寿を目指して運動が推奨されている。しかし、運動刺激が骨格筋に受容される仕組みは不明である。本研究の結果から、運動刺激は直接的に骨格筋細胞を刺激するだけでなく、神経を介して骨格筋細胞に伝達されることが示唆された。このことは、随意的な筋活動はもちろん、他動的な筋活動や骨格筋への刺激が運動効果をもたらすことを示すものであり、運動効果を増強しサルコペニア克服策確立のための知的基盤が形成できたと考えている。

Report

(7 results)
  • 2024 Annual Research Report   Final Research Report ( PDF )
  • 2023 Research-status Report
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (34 results)

All 2025 2024 2023 2022 2020 Other

All Int'l Joint Research (7 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] University of Minnesota Medical School(米国)

    • Related Report
      2024 Annual Research Report
  • [Int'l Joint Research] University of Minnesota Medical School(米国)

    • Related Report
      2023 Research-status Report
  • [Int'l Joint Research] University of Minnesota Medical School(米国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] University of Minnesota Medical School(米国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] King's College London(英国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] University of Minnesota Medical School(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] University of Minnesota Medical School(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Effect of Oral Lactate Administration on Skeletal Muscle Mass in Mice Under Different Loading Conditions2024

    • Author(s)
      OHNO YOSHITAKA、NAKATANI MASASHI、MATSUI YUKI、SUDA YOHEI、ITO TAKAFUMI、ANDO KOKI、YOKOYAMA SHINGO、GOTO KATSUMASA
    • Journal Title

      In Vivo

      Volume: 39 Issue: 1 Pages: 218-227

    • DOI

      10.21873/invivo.13820

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glycative stress inhibits hypertrophy and impairs cell membrane integrity in overloaded mouse skeletal muscle2024

    • Author(s)
      Egawa Tatsuro、Ogawa Takeshi、Yokokawa Takumi、Kido Kohei、Iyama Ryota、Zhao Haiyu、Kurogi Eriko、Goto Katsumasa、Hayashi Tatsuya
    • Journal Title

      Journal of Cachexia, Sarcopenia and Muscle

      Volume: - Issue: 3 Pages: 1-14

    • DOI

      10.1002/jcsm.13444

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 骨格筋の可塑性制御における温熱刺激の新しい生理機能2024

    • Author(s)
      後藤勝正
    • Journal Title

      細胞

      Volume: 56 Pages: 554-557

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impacts of myosin heavy chain phenotypes on recovery of leg extension force after ACL-reconstructed knee2023

    • Author(s)
      Kobayashi T、Ogura Y、Kishiro S、Kurosaka M、Yoshihara T、Kakigi R、Minakawa N、Uehara K、Suzuki T、Goto K、Niki H
    • Journal Title

      The Journal of Physical Fitness and Sports Medicine

      Volume: 12 Issue: 3 Pages: 77-85

    • DOI

      10.7600/jpfsm.12.77

    • ISSN
      2186-8123, 2186-8131
    • Year and Date
      2023-05-25
    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activation of Lactate Receptor Positively Regulates Skeletal Muscle Mass in Mice2023

    • Author(s)
      Ohno Y、Nakatani M、Ito T、Matsui Y、Ando K、Suda Y、Ohashi K、Yokoyama S、Goto K
    • Journal Title

      Physiological Research

      Volume: 72 Pages: 465-473

    • DOI

      10.33549/physiolres.935004

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique2023

    • Author(s)
      Goto Katsumasa、Ohashi Kazuya
    • Journal Title

      Methods in Molecular Biology

      Volume: 2460 Pages: 217-225

    • DOI

      10.1007/978-1-0716-3036-5_16

    • ISBN
      9781071630358, 9781071630365
    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Responses of neuromuscular properties to unloading and potential countermeasures during space exploration missions2022

    • Author(s)
      Ohira, T., F. Kawano, K. Goto, and Y. Ohira
    • Journal Title

      Neuroscience and Biobehavioral Reviews

      Volume: 136 Pages: 104617-104617

    • DOI

      10.1016/j.neubiorev.2022.104617

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Methylglyoxal reduces molecular responsiveness to 4 weeks of endurance exercise in mouse plantaris muscle2022

    • Author(s)
      Egawa Tatsuro、Ogawa Takeshi、Yokokawa Takumi、Kido Kohei、Goto Katsumasa、Hayashi Tatsuya
    • Journal Title

      Journal of Applied Physiology

      Volume: 132 Issue: 2 Pages: 477-488

    • DOI

      10.1152/japplphysiol.00539.2021

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fine-Tuning of Piezo1 Expression and Activity Ensures Efficient Myoblast Fusion during Skeletal Myogenesis2022

    • Author(s)
      Ortuste Quiroga Huascar Pedro、Ganassi Massimo、Yokoyama Shingo、Nakamura Kodai、Yamashita Tomohiro、Raimbach Daniel、Hagiwara Arisa、Harrington Oscar、Breach-Teji Jodie、Asakura Atsushi、Suzuki Yoshiro、Tominaga Makoto、Zammit Peter S.、Goto Katsumasa
    • Journal Title

      Cells

      Volume: 11 Issue: 3 Pages: 393-393

    • DOI

      10.3390/cells11030393

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Morphological changes in C2C12 myotubes following knockdown of mechanoreceptor Piezo12025

    • Author(s)
      Nakamura, M., Yogi, U., Akiyama, K., Kageyama, S., Yamada, M., Goto, A., Ohashi, K., Ito, R., Egawa, T., Kobayashi, K., Asakura, A., Goto, K.
    • Organizer
      第130回日本解剖学会、第102回日本生理学会、第98回日本薬理学会合同大会(APPW2025)
    • Related Report
      2024 Annual Research Report
  • [Presentation] Mild hyperbaric oxygen treatment promotes skeletal muscle regeneration through AMPK2025

    • Author(s)
      Fukunaga, T., Takemura, A., Suzuki, S., Iyama, R., Zhao, H., Fujino, R., Oba, M., Miura, S., Goto, K., Hayashi, T., Egawa, T.
    • Organizer
      第130回日本解剖学会、第102回日本生理学会、第98回日本薬理学会合同大会(APPW2025)
    • Related Report
      2024 Annual Research Report
  • [Presentation] Prevention of skeletal muscle dysfunction via activation of primary cilia by exercise-mimetic drugs2025

    • Author(s)
      Oba, M., Suzuki, S., Goto, K., Hayashi, T., Egawa, T.
    • Organizer
      第130回日本解剖学会、第102回日本生理学会、第98回日本薬理学会合同大会(APPW2025)
    • Related Report
      2024 Annual Research Report
  • [Presentation] A possible physiological role of sclerostin in aging-associated skeletal muscle atrophy2024

    • Author(s)
      Kobayashi, T., Yokoyama, S., Asakura, A., Goto, K.
    • Organizer
      American Physiology Summit 2024
    • Related Report
      2024 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リアノジン受容体3機能抑制によるfusogenic regulatorsの発現増加と巨大筋管細胞の形成2024

    • Author(s)
      伊藤理香、江川達郎、朝倉淳、後藤勝正
    • Organizer
      第78回日本体力医学会大会
    • Related Report
      2024 Annual Research Report
  • [Presentation] 骨格筋の概要と筋トレーニングのパラダイムシフト2024

    • Author(s)
      後藤勝正
    • Organizer
      第15回日本健康運動看護学会学術集会
    • Related Report
      2024 Annual Research Report
    • Invited
  • [Presentation] Ryanodine receptor 3 impacts on the expression of aminopeptidase in mammalian skeletal muscle cells during myogenic differentiation2023

    • Author(s)
      Ito R, Yokoyama S, Ohashi K, Hagiwara A, Takeo Y, Egawa T, Asakura A, Goto K
    • Organizer
      American Physiology Summit 2023
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research
  • [Presentation] 拡散型圧力波は不動化に伴う関節可動域制限ならびに筋萎縮からの回復を促進する2023

    • Author(s)
      横山真吾,塚田晋太朗,水谷仁一,後藤勝正
    • Organizer
      SHOCK WAVE JAPAN 2023
    • Related Report
      2023 Research-status Report
  • [Presentation] スクレロスチンが骨格筋に及ぼす影響2023

    • Author(s)
      小林哲士,横山真吾,後藤勝正,仁木久照
    • Organizer
      第38回日本整形外科学会基礎学術集会
    • Related Report
      2023 Research-status Report
  • [Presentation] 間欠的な高二酸化炭素分圧環境への曝露が骨格筋細胞の成長に及ぼす影響2023

    • Author(s)
      横田裕平,小寺紫雲,櫻井隆太郎,鈴木星矢,山下創大,山本竜矢,大橋和也,萩原ありさ,後藤勝正
    • Organizer
      第69回日本宇宙航空環境医学会大会
    • Related Report
      2023 Research-status Report
  • [Presentation] 終末糖化産物AGEsによるマウス骨格筋細胞膜脆弱化の誘導2023

    • Author(s)
      江川達郎,小川岳史,木戸康平,横川拓海,後藤勝正,林達也
    • Organizer
      第69回日本宇宙航空環境医学会大会
    • Related Report
      2023 Research-status Report
  • [Presentation] 新規運動抵抗性因子としてのメチルグリオキサールの可能性2023

    • Author(s)
      Egawa, T., Ogawa, T., Kido, K., Yokokawa, T., Goto, K., Hayashi, T.
    • Organizer
      日本生理学会 第100回記念大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Abnormal differentiation of C2C12 myoblasts due to low expression level of ryanodine receptor 32023

    • Author(s)
      Ito, R., Yokoyama, S., Ohashi, K., Hagiwara, A., Takeo, Y., Egawa, T., Asakura, A., Goto, K.
    • Organizer
      日本生理学会 第100回記念大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 胃-骨格筋間コミュニケーションの可能性-胃抑制性ペプチドによるC2C12筋管細胞の自動拍動-2022

    • Author(s)
      後藤勝正,横山真吾,大橋和也,萩原ありさ,青島惠,伊藤理香,江川達郎,朝倉淳
    • Organizer
      第77回日本体力医学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 胃抑制性ペプチドはマウスヒラメ筋の廃用性筋萎縮ならびに速筋化を軽減する― 蛍光免疫染色を用いた検討 ―2022

    • Author(s)
      横山真吾,大橋和也,萩原ありさ,伊藤理香,後藤勝正
    • Organizer
      第77回日本体力医学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 糖化ストレスは物理的負荷に伴う筋細胞膜損傷を増幅させる2022

    • Author(s)
      江川達郎,小川岳史,木戸康平,横川拓海,後藤勝正,林達也
    • Organizer
      第77回日本体力医学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] A possible of gastric inhibitory polypeptide in the regulation of skeletal muscle mass2020

    • Author(s)
      Goto, K., Aoshima, M., Hagiwara, A., Yamashita, T., Ortuste Quiroga, H.P., Yokoyama, S.
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Research-status Report
  • [Remarks] 豊橋創造大学大学院健康科学研究科生体機能学分野

    • URL

      https://sozo-ac.com/professor/goto_katsumasa/index.html

    • Related Report
      2023 Research-status Report 2022 Research-status Report

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Published: 2019-10-10   Modified: 2026-01-16  

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