Generation of MicroAntibody by directed evolution
| Project/Area Number |
20200033
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
Living organism molecular science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
TSUMURAYA Takeshi Osaka Prefecture University, 理学系研究科, 准教授 (00372855)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJII Ikuo 大阪府立大学, 理学系研究科, 教授 (70189984)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥33,020,000 (Direct Cost: ¥25,400,000、Indirect Cost: ¥7,620,000)
Fiscal Year 2010: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2008: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
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| Keywords | ペプチド / コンビナトリアル化学 / ポストゲノム創薬 / マイクロ抗体 / 分子プローブ / 分子標的医薬 / ライブラリー / タンパク質 / 生体分子 / バイオテクノロジー / 進化分子工学 / バクテリオファージ / 試験管内進化 / ケミカルバイオロジー / 免疫学 |
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| Research Abstract |
In this study, we have developed a method to downsize the antibody by the combination of structure-design of proteins and directed evolution. We have constructed a library of alternative-binding peptide with non-immunoglobulin domain to develop molecular probe and molecular targeting drug. The helix-loop-helix peptide, which binds to specifically target molecules, is named as "MicroAntibody". We have constructed a library of MicroAntibody to generate human IgG-Fc-specific peptides and Aurora kinase-specific peptides. We also studied immunogenicity and stability of MicroAntibody
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Report
(4 results)
Research Products
(89 results)
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
K.Tsumoto, A.Yokota, Y.Tanaka, M.Ui, T.Tsumuraya, I.Fujii, I.Kumagai, Y.Nagumo, H.Oguri, M.Inoue, M.Hirama
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Journal Title
J.Mol.Biol. 283
Pages: 12259-12266
Related Report
Peer Reviewed
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
Kouhei Tsumoto, Akiko Ybkota, Yoshikazu Tanaka, Mihoko Ui, Takeshi Tsumuraya, Ikuo Fujii, Izumi Kumagai, Yoko Nagumo, Hiroki Oguri, Masayuki Inoue, Masahiro Hirama
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Journal Title
Journal of Biological Chemistry 283
Pages: 12259-12266
Related Report
Peer Reviewed
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[Presentation] ホロ酵素型触媒抗体の分子設計2010
Author(s)
廣瀬仁孝, 石川文洋, 円谷健, 藤井郁雄
Organizer
第27回有機合成化学セミナー
Place of Presentation
シーサイドホテル舞子ビラ神戸(兵庫県)
Year and Date
2010-09-03
Related Report
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[Presentation]2009
Author(s)
R.El-Haggar, Z.Ye, Y.Ishino, K.Kamikawa, T.Tsumuraya, I.Fujii
Organizer
2^<nd> Switzerland-Japan Biomolecular Chemistry Symposium
Place of Presentation
東京大学(東京都)
Year and Date
2009-09-11
Related Report
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[Presentation] ホロ酵素型抗体酵素2009
Author(s)
石川文洋, 円谷健, 藤井郁雄
Organizer
2009年度酵素・補酵素研究会
Place of Presentation
奈良
Year and Date
2009-07-10
Related Report
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