Invention of a light-dependent nitrogenase from chlorophyll biosynthesis enzymes
Project/Area Number |
20200063
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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Allocation Type | Single-year Grants |
Research Field |
Applied biochemistry
Evolutionary biology
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Research Institution | Nagoya University |
Principal Investigator |
FUJITA Yuichi Nagoya University, 生命農学研究科, 准教授 (80222264)
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Co-Investigator(Renkei-kenkyūsha) |
KURISU Genji 大阪大学, 蛋白質研究所, 教授 (90294131)
OHKI Kaori 福井県立大学, 生物資源学部, 教授 (90101104)
TERAUCHI Kazuki 立命館大学, 生物科学部, 准教授 (70444370)
SHIMA Seigo マックスプランク研究所, グループリーダー
NOMATA Jiro 東京工業大学, 資源化学研究所, 助教 (40583216)
|
Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2008: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
Keywords | 光合成 / クロロフィル / プロトクロロフィリド / 酵素の進化 / プロトクロロフィリド還元酵素 / 単鎖デヒドロゲナーゼ/レダクターゼ / Synechocystis sp.PCC6803 / Synechococcus sp.CC9913 / 光依存型プロトクロロフィリド還元酵素 / ラン藻(シアノバクテリア) / 短鎖アルコールデヒドロゲナーゼファミリー / クロロフィル生合成 / プロラメラ体 |
Research Abstract |
Light-dependent protochlorophyllide oxidoreductase (LPOR) belongs to a large enzyme family "short-chain dehydrogenase/reductase". Toward the invention of new light-dependent enzymes it is very important to understand how the light-dependent property of LPOR has been evolved. I have identified a most diverged LPOR in a marine cyanobacterium by biochemical method and determined its enzyme parameters.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Electronic structures of a [4Fe-4S] cluster, [Fe_4S_4 (SCH_3)_3(CH_3COO)], in dark-operative protochlorophyllide reductase (DPOR).2011
Author(s)
Takano, Y., Yonezawa, Y., Fujita, Y., Kurisu, G., Nakamura, H.
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Journal Title
Chem.Phys.Lett. 503
Pages: 296-300
Related Report
Peer Reviewed
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[Journal Article] Electronic structures of a [4Fe-4S] cluster, [Fe_4S_4 (SCH_3)_3(CH_3COO)], in dark-operative protochlorophyllide reductase (DPOR).2011
Author(s)
Takano, Y., Yonezawa, Y., Fujita, Y., Kurisu, G., Nakamura, H.
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Journal Title
Chemical Physics Letters
Volume: 503
Pages: 296-300
Related Report
Peer Reviewed
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[Journal Article] Chlorophyll biosynthesis : spotlight on protochlorophyllide reduction.2010
Author(s)
Reinbothe, C., El Bakkouri, M., Buhr, F., Muraki, N., Nomata, J., Kurisu, G., Fujita, Y., Reinbothe, S.
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Journal Title
Trends Plant Sci. 15
Pages: 614-624
Related Report
Peer Reviewed
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[Journal Article] Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga Cyanidioschyzon merolae : Genetic evidence for nitrite reduction by a sulfite reductase-like enzyme.2010
Author(s)
Imamura, S., Terashita, M., Ohnuma, M., Maruyama, S., Minoda, A., Weber, A.P.M., Inouye, T., Sekine, Y., Fujita, Y., Omata, T., Tanaka, K.
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Journal Title
Plant Cell Physiol. 51
Pages: 707-717
NAID
Related Report
Peer Reviewed
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[Journal Article] X-ray crystal structure of the light-independent protochlorophyllide reductase.2010
Author(s)
Muraki, N., Nomata, J., Ebata, K., Mizoguchi, T., Shiba, T., Tamiaki, H., Kurisu, G., Fujita, Y.
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Journal Title
Nature 465
Pages: 110-114
Related Report
Peer Reviewed
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[Journal Article] Chlorophyll biosynthesis : spotlight on protochlorophyllide reduction.2010
Author(s)
Reinbothe, C., El Bakkouri, M., Buhr, F., Muraki, N., Nomata, J., Kurisu, G., Fujita, Y., Reinbothe, S.
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Journal Title
Trends in Plant Science
Volume: 15
Pages: 614-624
Related Report
Peer Reviewed
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[Journal Article] Nitrate assimilatory genes and their transcriptional regulation in a unicellular redalga Cyanidioschyzon merolae : Genetic evidence for nitrite reduction by a sulfite reductase-like enzyme.2010
Author(s)
Imamura, S., Terashita, M., Ohnuma, M., Maruyama, S., Minoda, A., Weber, A.P.M., Inouye, T., Sekine, Y., Fujita, Y., Omata, T., Tanaka, K.
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Journal Title
Plant and Cell Physiology
Volume: 51
Pages: 707-717
NAID
Related Report
Peer Reviewed
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[Journal Article] X-ray crystal structure of the light-independent protochlorophyllide reductase.2010
Author(s)
Muraki, N., Nomata, J., Ebata, K., Mizoguchi, T., Shiba, T., Tamiaki, H., Kurisu, G., Fujita, Y.
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Journal Title
Nature
Volume: 465
Pages: 110-114
Related Report
Peer Reviewed
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[Journal Article] Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis.2009
Author(s)
Masuda, S., Ikeda, R., Masuda, T., Hashimoto, H., Tsuchiya, T., Kojima, H., Nomata, J., Fujita, Y., Mimuro, M., Ohta, H., Takamiya, K.
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Journal Title
Plant J. 58
Pages: 952-960
Related Report
Peer Reviewed
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[Journal Article] Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis2009
Author(s)
Masuda, S., Ikeda, R., Masuda, T., Hashimoto, H., Tsuchiya, T., Kojima, H., Nomata, J., Fujita. Y., Mimuro, M., Ohta, H. and Takamiya, K
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Journal Title
Related Report
Peer Reviewed
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[Presentation] Characterization of nitrogenase-like enzyme, dark-operative protochlorophyllide oxidoreductase.2009
Author(s)
Nomata, J., Muraki, N., Ebata, K., Harimoto, J., Mizoguchi, T., Tamiaki, H., Kurisu, G., Fujita, Y.
Organizer
16^<th> International Conference on Nitrogen Fixation
Place of Presentation
Big Sky, MT, USA
Related Report
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[Presentation] Characterization of metal clusters of nitrogenase like dark-operative protochlorohyllide reductase and chlorophyllide reductase from Rhodobacter capsulatus2009
Author(s)
Nomata, J, Ebata, K, Muraki, N, Kitashima, M, Inoue, K, Kurisu, G, Fujita, Y
Organizer
14^<th> International Conference on Bioinorganic Chemistry
Place of Presentation
Nagoya Congress Center, Nagoya, Aichi
Related Report
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