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Development and application of real-time molecular microscope based on non-linear optical effect for biological specimen

Research Project

Project/Area Number 20240044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

ARAKI Tsutomu  Osaka University, 大学院・基礎工学研究科, 教授 (50136214)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Mamoru  大阪大学, 大学院・基礎工学研究科, 准教授 (70237949)
FUKUSHIMA Shuichirou  大阪大学, 大学院・基礎工学研究科, 助教 (40362644)
KINOーOKA Masahiro  大阪大学, 大学院・基礎工学研究科, 教授 (40234314)
IWATA Tetsuo  徳島大学, 大学院・ソシオテクノサイエンス研究部, 教授 (50304548)
YASUI Takeshi  徳島大学, 大学院・ソシオテクノサイエンス研究部, 教授 (70314408)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥50,830,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥11,730,000)
Fiscal Year 2010: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2009: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2008: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000)
Keywords分子イメージング / SHG顕微鏡 / CARS顕微鏡 / ラマン分光 / コラーゲン / 皮膚 / 非線形光学効果 / 細胞培養 / 非線形光学現象 / リポゾーム / コラーゲン分子配向
Research Abstract

Aims of this research project are to develop new microscopes utilizing non-linear optical effect induced by an ultra-short pulse laser and to observe molecular imaging of the biological specimen without unwanted staining treatment. We have developed a high-quality SHG microscope and CARS microscope, and following results have been obtained using these microscopes : (1) Age-dependency of collagen distribution in human skin was observed with the SHG microscope. This microscope was also applied to evaluate quality of cartilage cultured in collagen gel. (2) CARS image of Hela cell was demonstrated. Also repair of cell membrane was traced in time based on the CARS images.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (81 results)

All 2011 2010 2009 2008 Other

All Journal Article (23 results) (of which Peer Reviewed: 19 results) Presentation (53 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Real-time imaging of laser-induced membrane disruption oliving cell observed with multifocus coherent anti-Stokes Raman scattering microscopy2011

    • Author(s)
      南川丈夫, 新岡宏彦, 荒木勉, 橋本守
    • Journal Title

      J.Biomedical Optics Vol.16

      Pages: 21111-21111

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time imaging of laser-induced membrane disruption of a living cell observed with multifocus coherent anti-Stokes Raman scattering microscopy2011

    • Author(s)
      T.Minamikawa, H.Niioka, T.Araki, M.Hashimoto
    • Journal Title

      J.Biomedical Optics

      Volume: 16 Pages: 21111-21111

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイムCARS顕微鏡の開発と生細胞観測への応用2010

    • Author(s)
      橋本守, 南川丈夫, 新岡宏彦, 荒木勉
    • Journal Title

      日本レーザー医学会誌日本レーザー医学会誌 30巻

      Pages: 421-426

    • NAID

      10026089120

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ラジアル偏光ビームを用いたSHG顕微計測2010

    • Author(s)
      新岡宏彦, 薦田幸一郎, 吉木啓介, 荒木勉, 橋本守
    • Journal Title

      光学

      Volume: 39 Pages: 401-403

    • NAID

      10026586276

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイムCARS顕微鏡の開発と生細胞観測への応用2010

    • Author(s)
      橋本守, 南川丈夫, 新岡宏彦, 荒木勉
    • Journal Title

      日本レーザー医学会誌

      Volume: 30 Pages: 421-426

    • NAID

      10026089120

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time molecular imaging of organelles in living cell by multifocus excitation CARS microscope2010

    • Author(s)
      T.Minamikawa
    • Journal Title

      Proc.SPIE 7569

      Pages: 756927-756927

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed CARS spectral imaging using acousto optic tunable filter2010

    • Author(s)
      M.Hashimoto
    • Journal Title

      Proc.SPIE 7569

      Pages: 75690-75690

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autoregressive-model-based fluorescence-lifetime measurements byphase-modulation fluorometry using a pulsed-excitation light source and a high-gain photomultiplier tube2009

    • Author(s)
      T.Iwata, R.Ito, Y.Mizutani, T.Araki
    • Journal Title

      Applied Spectroscopy Vol.63

      Pages: 256-1261

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A proposal of a pseudo-lock-in light-detection scheme by sinumodulation of bias voltages applied to tdynodes in a photomultiplier tube2009

    • Author(s)
      T.Iwata, H.Ochil, T.Araki
    • Journal Title

      Meas.Sci.Technol. Vol.20

      Pages: 65901-65907

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging2009

    • Author(s)
      T.Minamikawa, M.Hashimoto, K. Fujita, S.Kawata, T.Araki
    • Journal Title

      Opt.Express Vol.17

      Pages: 9526-9536

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ex vivo ain vivo second-harmonic-generation imaging of dermal collagen fiber in skin : comparison of imaging characteristicsbetween mode-locked Cr : Forsterite and Ti : Saphire lasers2009

    • Author(s)
      T.Yasui, Y.Takahashi, M.Ito, S.Fukushima, T.Araki
    • Journal Title

      Appl.Opt. Vol.48

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of second harmonic generation from self-assembled monolayers on gold by excitation with radially polarized beam2009

    • Author(s)
      M.Hashimoto, K.Ashida, K.Yoshiki, T.Araki
    • Journal Title

      Opt.Lett. Vol.34

      Pages: 1423-1425

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Observation of dermal collagen fiber in wrinkled skin usingpolarization-resolved secondharmonic-generation microscopy2009

    • Author(s)
      T.Yasui, Y.Takahashi, S.Fukushima Y.Ogura, T.Yamashita, T.Kuwahara,T.Hirao, T.Araki
    • Journal Title

      Optics Express Vol.17

      Pages: 912-92

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A proposal of a pseudo-lock-in light-detection scheme by sinusoidal modulation of bias voltages applied to two dynodes in a photomultiplier tube2009

    • Author(s)
      T.Iwata
    • Journal Title

      Meas.Sci.Technol 20

      Pages: 65901-8

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multi-focus excitation coherent anti-Stokes Raman scattering (CARS) microscopy and its applications for real-time imaging2009

    • Author(s)
      T.Minamikawa
    • Journal Title

      Optics Express 17

      Pages: 9526-9534

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multifocus CARS microscopy for realtime vibrational imaging2009

    • Author(s)
      M.Hashimoto
    • Journal Title

      Proc.SPIE 7507

      Pages: 75070-75070

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] "多焦点CARS顕微鏡によるリアルタイム分子イメージング2009

    • Author(s)
      南川丈夫
    • Journal Title

      オプトロニクス 28

      Pages: 103-107

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 生体組織の無染色顕微鏡観察術2009

    • Author(s)
      橋本守
    • Journal Title

      光アライアンス 20

      Pages: 24-28

    • Related Report
      2009 Annual Research Report
  • [Journal Article] SHGイメージングとCARSイメージング2009

    • Author(s)
      荒木勉
    • Journal Title

      ぶんせき 9号

      Pages: 409-495

    • NAID

      10025623525

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      井上康志, 川田善正, 渡慶次学, 橋本守, 火原彰秀
    • Journal Title

      顕微分光法ナノ・マイクロの世界を見る分光法(講談社サイエンティフィック)

      Pages: 55-76

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Ex vivo and in vivo second-harmonic-generation imaging of dermal collagen fiber in skin : comparison of imaging characteristics between mode-locked Cr : Forsterite and Ti : Sapphire lasers2009

    • Author(s)
      T. Yasui
    • Journal Title

      Applied Optics 48

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Observation of dermal collagen fiber in wrinkled skin using polarization-resolved secondharmonic-generation microscopy2009

    • Author(s)
      T. Yasui
    • Journal Title

      Optics Express 17

      Pages: 912-923

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A deconvolution procedure for determination of a fluorescence decay waveform applicable to a band-limited measurement system that has a time delay2008

    • Author(s)
      T. Iwata
    • Journal Title

      Meas. Sci. Technol. 19

      Pages: 15601-15601

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 非線形ラマシ散乱顕微鏡によるリアルタイムイメージング2011

    • Author(s)
      橋本守
    • Organizer
      第58回応用物理学関連連合講演会,シンポジウム「ラマン分光イメージングが拓く新しいサイエンスとテクノロジー」
    • Place of Presentation
      神奈川工科大学(厚木市)(招待講演)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 極短パルスレーザーによる真皮コラーゲンの可視化2011

    • Author(s)
      荒木勉
    • Organizer
      医工連携をめざした生体光計測」フォーラム
    • Place of Presentation
      徳島大学(徳島市)(招待講演)
    • Year and Date
      2011-03-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体コラーゲン顕微鏡を用いたin vivo皮膚計測2011

    • Author(s)
      安井武史
    • Organizer
      第44回光学五学会関西支部連合講演会「光でみる生体情報」
    • Place of Presentation
      大阪市立大学文化交流センター(大阪市)(招待講演)
    • Year and Date
      2011-01-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 培養軟骨作成過程における細胞分布の非侵襲計測2011

    • Author(s)
      前原鈴子, 福島修一郎, 紀ノ岡正博, 荒木勉
    • Organizer
      日本機械学会 第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2011-01-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 第2高調波発生顕微鏡を用いたコラーゲン熱変陸のモニタリング2011

    • Author(s)
      田仲亮介, 安井武史, 福島修一郎, 荒木勉
    • Organizer
      日本機械学会 第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 多焦点CARS顕微鏡による細胞応答の無染色高速観測2010

    • Author(s)
      橋本守
    • Organizer
      平成22年度ナノ分光部会・生細胞分光部会 合同シンポジウム「分光学が拓く新しいイメージング・イメージングが拓く新しい分光学」
    • Place of Presentation
      東京大学(東京)(招待講演)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vivo observation of dermal collagen fiber in human skin using a second harmonic generation microscope2010

    • Author(s)
      荒木勉
    • Organizer
      WINPTech 2010
    • Place of Presentation
      神戸大学(神戸市)招待講演
    • Year and Date
      2010-12-02
    • Related Report
      2010 Final Research Report
  • [Presentation] In vivo observation of dermal collagen fiber in human skin using a second harmonic generation microscope2010

    • Author(s)
      T.Araki
    • Organizer
      WINPTech2010
    • Place of Presentation
      神戸大学(神戸市)(招待講演)
    • Year and Date
      2010-12-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 第2高調波発生光を用いたコラーゲンゲルの局所的変位場の解析2010

    • Author(s)
      斉藤徹也, 福島修一郎, 荒木勉
    • Organizer
      日本機械学会 第21回バイオフロンティア講演会
    • Place of Presentation
      ITビジネスプラザ武蔵(金沢市)
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体コラーゲンSHG顕微鏡を用いたヒト皮膚のin vivo観察2010

    • Author(s)
      安井武史, 米津真人, 山下豊信, 小倉有紀, 平尾哲二, 荒木勉
    • Organizer
      Optics & Photonics Japan 2010
    • Place of Presentation
      中央大学(東京都)
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 偏光分布制御CARS顕微鏡を用いた液晶分子の3次元配向イメージング2010

    • Author(s)
      池田晃平, 高木達朗, 南川丈夫, 新岡宏彦, 橋本守, 荒木勉
    • Organizer
      平成22年度日本分光学会年次講演会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pt基板上自己組織化単分子膜の高感度/高分解能SHGイメージング2010

    • Author(s)
      新岡宏彦, 蘆田幸一郎, 吉木啓介, 荒木勉, 橋本守
    • Organizer
      平成22年度日本分光学会年次講演会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 多焦点CARS顕微鏡における生細胞へのフォトダメージ評価2010

    • Author(s)
      南川丈夫, 村上貴視, 松村直和, 新岡宏彦, 福島修一郎, 荒木勉, 橋本守
    • Organizer
      平成22年度日本分光学会年次講演会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体コラーゲンSHG顕微鏡を用いた光学的熱傷診断に関する研究2010

    • Author(s)
      田仲亮介, 安井武史, 福島修一郎, 荒木勉
    • Organizer
      Optics & Photonics Japan 2010
    • Place of Presentation
      中央大学(東京都)
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 皮膚コラーゲンを可視化する新しい光学顕微鏡2010

    • Author(s)
      荒木勉
    • Organizer
      岡山県医用工学研究会
    • Place of Presentation
      岡山大学(岡山市)(招待講演)
    • Year and Date
      2010-10-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Noninvasive estimation of engineered cartilage using second-harmonic-generation microscopy2010

    • Author(s)
      R.Maehara, S.Fukushima, M.Kino-oka, T.Araki
    • Organizer
      1st International Conference on Applied Bionics and Biomechanics
    • Place of Presentation
      Venice(Italy)
    • Year and Date
      2010-10-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] ラジアル偏光励起第二高調波顕微鏡による強誘電性液晶のスイッチング挙動観測の数値解析2010

    • Author(s)
      橋本守, 大久保隆行, 新岡宏彦, 荒木勉
    • Organizer
      第61回応用物理学会学術講演会
    • Place of Presentation
      長崎大学(長崎市)(招待講演)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pt基板上自己組織化分子膜のラジアル偏光励起高分解・増強SHGイメージング2010

    • Author(s)
      新岡宏彦, 蘆田幸一郎, 吉木啓介, 荒木勉, 橋本守
    • Organizer
      第61回応用物理学会学術講演会
    • Place of Presentation
      長崎大学
    • Year and Date
      2010-09-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Visualization of arterial calcification by X-ray computed tomography2010

    • Author(s)
      S.Fukushima, T.Araki
    • Organizer
      10th International Congress of Physiological Anthropology
    • Place of Presentation
      Fremantle(Australia)
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Second-harmonic-generation microscopy for quality evaluation of extracellular matrix2010

    • Author(s)
      福島修一郎, 前原鈴子, 紀ノ岡正博, 荒木勉
    • Organizer
      23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology
    • Place of Presentation
      北海道大学(札幌市)招待講演
    • Year and Date
      2010-09-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Second-harmonic- generation microscopy for quality evaluation of extracellular matrix2010

    • Author(s)
      S.Fukushima, R.Maehara, M.Kino-oka, T.Araki
    • Organizer
      23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology
    • Place of Presentation
      北海道大学(札幌市)(招待講演)
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Visualization of collagen molecule orientation in wrinkled skin using apolarization-resolved second-harmonic-generation microscope2010

    • Author(s)
      T.Yasui, T.Araki
    • Organizer
      30th European Congress of Molecular Spectroscopy(EUCMOS2010)
    • Place of Presentation
      Florence (Italy)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] ラジアル偏光励起SHG顕微鏡によるPt基板上自己組織化 単分子膜の高分解・増強イメージング2010

    • Author(s)
      新岡奉彦, 蘆田幸一廊, 吉木啓介, 荒木勉, 橋本守
    • Organizer
      レーザ顕微鏡研究会 第36回講演会
    • Place of Presentation
      理化学研究所(和光市)
    • Year and Date
      2010-07-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Determination burn depth based on depth-resolved second-harmonic-generation imagdermal collagen2010

    • Author(s)
      T.Yasui, K.Sasaki, R.Tanaka, S.Fukushima, T.Araki
    • Organizer
      Biomedical Optics (OSA Topical Meeting and Tabletop Exhibit)
    • Place of Presentation
      Miami (USA)
    • Year and Date
      2010-04-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Determination of burn depth based on depth-resolved second-harmonic-generation imaging of dermal collagen2010

    • Author(s)
      T.Yasui, K.Sasaki, R.Tanaka, S.Fukushima, T.Araki
    • Organizer
      Biomedical Optics (OSA Topical Meeting and Tabletop Exhibit)
    • Place of Presentation
      Miami(USA)
    • Year and Date
      2010-04-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 偏光分布制御CARS顕微鏡による液晶の3次元配向イメージング2010

    • Author(s)
      高木達朗
    • Organizer
      レーザー学会学術講演会
    • Place of Presentation
      千里ライフサイエンスセンター,大阪
    • Year and Date
      2010-02-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] リアルタイムCARS顕微鏡による細胞の膜修復過程の観測2010

    • Author(s)
      橋本守
    • Organizer
      レーザー学会学術講演会第30回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター,大阪
    • Year and Date
      2010-02-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] リアルタイムCARS顕微鏡による細胞の膜修復過程の観測2010

    • Author(s)
      橋本守, 南川丈夫, 新岡宏彦, 荒木勉
    • Organizer
      レーザー学会学術講演会第30回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(吹田市)招待講演
    • Year and Date
      2010-02-02
    • Related Report
      2010 Final Research Report
  • [Presentation] CARS顕微鏡の開発と歪み分布可視化の可能性2010

    • Author(s)
      橋本守
    • Organizer
      日本実験力学会ナノ材料計測分科会第1回研究会
    • Place of Presentation
      兵庫県立大学、姫路
    • Year and Date
      2010-01-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 偏光分解SHG顕微鏡を用いた生体軟組織コラーゲン繊維配向の定量的解析2010

    • Author(s)
      小石洋平
    • Organizer
      第22回バイオエンジニアリング講演会
    • Place of Presentation
      岡山理科大学、岡山市
    • Year and Date
      2010-01-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] レーザーによる生体分子可視化の試み -染めずに、すばやく、あるがまま-2010

    • Author(s)
      荒木勉
    • Organizer
      第22回バイオエンジニアリング講演会
    • Place of Presentation
      岡山理科大学、岡山市
    • Year and Date
      2010-01-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] レーザー誘起アブレーションによる細胞の膜破壊と膜修復過程のリアルタイムCARSイメージング2009

    • Author(s)
      南川丈夫
    • Organizer
      日本光学会年次学術講演会
    • Place of Presentation
      朱鷺メッセ,新潟
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Imaging of self-assembled monolayer on Pt substrate with radial beam excited SHG microscopy2009

    • Author(s)
      H.Niioka
    • Organizer
      平成21年度日本分光学会年次講演会
    • Place of Presentation
      東京工業大学、東京
    • Year and Date
      2009-11-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Real-time CARS imaging of local disruption and reaction of living cell induced by near-infrared laser ablation2009

    • Author(s)
      T.Minamikawa
    • Organizer
      平成21年度日本分光学会年次講演会
    • Place of Presentation
      東京工業大学、東京
    • Year and Date
      2009-11-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 第2高調波発生顕微鏡による培養軟骨の品質評価2009

    • Author(s)
      前原鈴子, 福島修一郎, 紀ノ岡正博, 荒木勉
    • Organizer
      日本機械学会第20回バイオフロンティア講演会, A208
    • Place of Presentation
      和歌山県民文化会館(和歌山市)
    • Year and Date
      2009-11-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 第2高調波発生顕微鏡による軟骨細胞の品質評価2009

    • Author(s)
      前原鈴子
    • Organizer
      第20回バイオフロンティア講演会
    • Place of Presentation
      和歌山県民文化会館
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Realtime biomolecular imaging by multifocus CARS microscopy2009

    • Author(s)
      M.Hashimoto
    • Organizer
      International workshop on NanoBioPhotonics
    • Place of Presentation
      Marseille, France
    • Year and Date
      2009-10-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] High-speed non-staining biomolecular imaging by nonlinear Raman microspectroscopy2009

    • Author(s)
      橋本守
    • Organizer
      10th Polish-Japanese Seminar on Biomedical Engineering
    • Place of Presentation
      Warsaw(Poland)招待講演
    • Year and Date
      2009-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] High-speed non-staining biomolecular imaging by nonlinear Raman microspectroscopy2009

    • Author(s)
      M.Hashimoto
    • Organizer
      10th Polish-Japanese Seminar on Biomedical Engineering
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] ラジアル偏光を用いたpt基板上自己組織化分子膜の顕微増強SHGイメージング2009

    • Author(s)
      新岡宏彦
    • Organizer
      第70回応用物理学会学術講演会
    • Place of Presentation
      富山大学,富山市
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多焦点CARS顕微鏡によるリアルタイム細胞イメージング2009

    • Author(s)
      南川丈夫
    • Organizer
      第70回応用物理学会学術講演会
    • Place of Presentation
      富山大学,富山市
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Orientation mapping of dermal collagen fiber in photoaged skin using polarization-resolved second-harmonic-generation microscopy2009

    • Author(s)
      T.Yasui
    • Organizer
      11th International Conference on Methods and Applications of Fluorescence : Spectroscopy, Imaging and Probes (MAF-11),
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2009-09-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] Observation of dermal collagen fiber in wrinkled skin using a second harmonic-generation microscope2009

    • Author(s)
      T.Araki
    • Organizer
      3rd Switzerland-Japan Workshop on Biomechanics
    • Place of Presentation
      Engelberg, Switzeralnad
    • Year and Date
      2009-09-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Polarization-resolved second-harmonic-generation imaging of photoaged dermal collagen fiber2009

    • Author(s)
      M.Yonetsu
    • Organizer
      8th Pacific Rim Conference on Lasers and Electro-Optics (CLEO/Pacific Rim 2009)
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2009-09-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生きた細胞のリアルタイムCARSイメージング2009

    • Author(s)
      南川丈夫
    • Organizer
      レーザ顕微鏡研究会 第35回講演会
    • Place of Presentation
      理化学研究所 和光市
    • Year and Date
      2009-07-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] CARS顕微鏡の多焦点化によるリアルタイムイメージング2009

    • Author(s)
      橋本守
    • Organizer
      第48回日本生体医工学会大会
    • Place of Presentation
      東京都江戸川区 タワーホール船堀、東京
    • Year and Date
      2009-04-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Observation of collagen fibril structure by second-harmonic generation microscopy2009

    • Author(s)
      Y.Koishi
    • Organizer
      4th Asian Pacific Conference on Biomechanics
    • Place of Presentation
      Christchurch, New Zealand
    • Year and Date
      2009-04-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Polarization-resolved second-harmonic-generation imaging of photoaged dermal collagen fiber2009

    • Author(s)
      T. Yasui
    • Organizer
      BiOS 2009 (Photonic West 2009)
    • Place of Presentation
      San Jose, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Three-dimensional single molecular orientation measurement by built-in type compact polarization-mode-selector2009

    • Author(s)
      K. Yoshiki
    • Organizer
      BiOS 2009 (Photonic West 2009)
    • Place of Presentation
      San Jose, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] 3次元偏光制御を用いたベクトル顕微鏡2009

    • Author(s)
      橋本守
    • Organizer
      レーザー学会学術講演会第29回年次大会
    • Place of Presentation
      徳島大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] 第二高調波発生光を用いた関節軟骨コラーゲンの定量的イメージング2009

    • Author(s)
      小石洋平
    • Organizer
      第21回バイオエンジニアリング講演会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2008 Annual Research Report
  • [Presentation] 偏光分解SHG(第2 高調波発生光)顕微鏡を用いた光老化真皮におけるコラーゲン線維変性の観察2008

    • Author(s)
      高橋由
    • Organizer
      Optics & Photonics Japan 2008
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2008 Annual Research Report
  • [Presentation] 多焦点CARS顕微鏡によるDOPC/DPPC混合脂質リポソームの脂質分布計測2008

    • Author(s)
      南川丈夫
    • Organizer
      第69回応用物理学会学術講演会
    • Place of Presentation
      中部大学
    • Related Report
      2008 Annual Research Report
  • [Book] 近接場光のセンシング・イメージング技術への応用2010

    • Author(s)
      荒木勉
    • Publisher
      シーメムシー出版
    • Related Report
      2010 Final Research Report
  • [Book] 近擦場光のセンシング・イメージング技術への応用2010

    • Author(s)
      荒木勉(共著)
    • Publisher
      シーメムシー出版
    • Related Report
      2010 Annual Research Report
  • [Book] 顕微分光法ナノ・マイクロの世界を見る分光法2009

    • Author(s)
      橋本守
    • Publisher
      講談社サイエンティフィック
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://sml.me.es.osaka-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://sml.me.es.osaka-u.ac.jp/araki_lab/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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