| Project/Area Number |
20247010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KANAHO Yasunori University of Tsukuba, 大学院・人間総合科学研究科, 教授 (00214437)
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| Co-Investigator(Renkei-kenkyūsha) |
HASEGAWA Hiroshi 筑波大学, 大学院・人間総合科学研究科, 助教 (10332230)
FUNAKOSHI Yuji 筑波大学, 大学院・人間総合科学研究科, 助教 (30415286)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2010: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2009: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2008: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
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| Keywords | PIP5-キナーゼ / ホスホリパーゼD / アダプター蛋白質 / クラスリン依存的エンドサイトーシス / NMDA受容体 / 長期抑制 / 好中球 / 精子形成 / PIP5K / NMDA / AMPA受容体 / 海馬神経細胞 / スパイン / AP-2 / クラスリン / エンドサイトーシス / PLD / ARF6 / 酵素放出反応 / 活性酸素 / エタノール / 脂質性シグナル伝達 / リン脂質代謝酵素 / ノックアウトマウス / パートナー蛋白質 / シグナル伝達 / 生殖 |
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| Research Abstract |
In this study, the physiological functions of lipid signaling molecule-producing enzymes, PIP5K and PLD, have been investigated. It was found that PIP5Kγ661 is dephosphorylated upon NMDA stimulation of hippocampal neurons, interacts with the adaptor complex AP-2 and stimulates the endocytosis of AMPA receptors, thereby inducing the long-term depression. On the other hand, PIP5Kα and PIP5Kβ were found to regulate spermatogenesis. Lastly, it was suggested that PLD is not involved in the neutrophil functions, such as superoxide generation and enzyme release, although the involvement of PLD in these neutrophil functions have been reported.
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