Physiological functions of lipid signaling molecule-producing enzymes based on their search of partner proteins
| Project/Area Number |
20247010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KANAHO Yasunori University of Tsukuba, 大学院・人間総合科学研究科, 教授 (00214437)
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| Co-Investigator(Renkei-kenkyūsha) |
HASEGAWA Hiroshi 筑波大学, 大学院・人間総合科学研究科, 助教 (10332230)
FUNAKOSHI Yuji 筑波大学, 大学院・人間総合科学研究科, 助教 (30415286)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2010: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2009: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2008: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
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| Keywords | PIP5-キナーゼ / ホスホリパーゼD / アダプター蛋白質 / クラスリン依存的エンドサイトーシス / NMDA受容体 / 長期抑制 / 好中球 / 精子形成 / PIP5K / NMDA / AMPA受容体 / 海馬神経細胞 / スパイン / AP-2 / クラスリン / エンドサイトーシス / PLD / ARF6 / 酵素放出反応 / 活性酸素 / エタノール / 脂質性シグナル伝達 / リン脂質代謝酵素 / ノックアウトマウス / パートナー蛋白質 / シグナル伝達 / 生殖 |
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| Research Abstract |
In this study, the physiological functions of lipid signaling molecule-producing enzymes, PIP5K and PLD, have been investigated. It was found that PIP5Kγ661 is dephosphorylated upon NMDA stimulation of hippocampal neurons, interacts with the adaptor complex AP-2 and stimulates the endocytosis of AMPA receptors, thereby inducing the long-term depression. On the other hand, PIP5Kα and PIP5Kβ were found to regulate spermatogenesis. Lastly, it was suggested that PLD is not involved in the neutrophil functions, such as superoxide generation and enzyme release, although the involvement of PLD in these neutrophil functions have been reported.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Phosphatidylinositol-4-Phosphate-5-Kinase alpha Deficiency Alters Dynamics of Glucose-Stimulated Insulin Release to Improve Glucohomeostasis and Decrease Obesity in Mice.2011
Author(s)
Huang P., Yeku O., Zong H., Tsang P., SuW., Yu X., Teng S., Osisami M, Kanaho Y., Pessin J., Frohman MA.
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Journal Title
Diabetes 60
Pages: 454-463
Related Report
Peer Reviewed
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[Journal Article] SIRT1 regulates thyroid-stimulated hormone release by enhancing PIP5Kgamma activity through deacylation of specific lysine residues in mammals.2010
Author(s)
Akieda-Asai S., Zaima N., Ikegami K., Kahyo T., Yao I., Hatanaka T., Iemura S., Sugiyama R., Yokozeki T., Eishi Y., Koike M., Ikeda K., Chiba T., Yamaza H., Shinokawa I., Song SY., Matsuno A., Mizutani A., Sawabe M., Chao MV., Tanaka M., Kanaho Y., Natsume T., Sugimura H., Date Y., McBurney MW., Guarente L., Setou M.
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Related Report
Peer Reviewed
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[Journal Article] Sequential regulation of DOCK2 dynamics by two phospholipids during neutrophil chemotaxis.2009
Author(s)
Nishikimi A., Fukuhara H., Su W., Hongu T., Takasuga S., Mihara H., Cao Q., Sanematsu F., Kanai M., Hasegawa H., Tanaka Y., Shibasaki M., Kanaho Y., Sasaki T., Frohman M.A., Fukui Y.
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Journal Title
Science 324
Pages: 384-387
Related Report
Peer Reviewed
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[Journal Article] Loss of PIP5KIβ demonstrates that PIP5KI isoform-specific PIP 2 synthesis is required for IP 3 formation.2008
Author(s)
Wang Y., Chen X., Lian L., Tang T., Stalker TJ., Sasaki T., Kanaho Y., Brass LF., Choi J.K., Hartwig J.H., Abrams C.S.
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Journal Title
Proc.Natl.Acad.Sci.USA. 105
Pages: 14064-16069
Related Report
Peer Reviewed
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[Journal Article] Loss of PIP5KIγ, unlike other PIP5KI isoforms, impairs the integrity of the membrane cytoskeleton in murine megakaryocytes.2008
Author(s)
Wang Y., Litvinov RI., Chen X., Bach TL., Lian L., Petrich B.G., Monkley S.J., Kanaho Y., Critchley D.R., Sasaki T., Birnbaum M.J., Weisel J.W., Hartwig J., Abrams C.S.
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Journal Title
J.Clin.Invest. 118
Pages: 812-819
Related Report
Peer Reviewed
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[Presentation] The role of PIP5K in wound healing.2009
Author(s)
Okada R., Hasegawa H., Furuya Y., Funakoshi Y., Kanaho Y.
Organizer
The 5^<th>Japan-Korea Conference in Cellular Signaling for Young Scientists.
Place of Presentation
ハウステンボス(長崎)
Year and Date
2009-11-25
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