Analysis of antigen-uptake network at mucosal epithelial layer

• Project/Area Number: 20249028
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: The University of Tokyo
• Principal Investigator: KIYONO Hiroshi The University of Tokyo, 医科学研究所, 教授 (10271032)
• Co-Investigator(Renkei-kenkyūsha): KUNISAWA Jun 東京大学, 医科学研究所, 講師 (80376615) ; SATO Shintaro 東京大学, 医科学研究所, 助教 (80447333) ; YUKI Yoshikazu 東京大学, 医科学研究所, 助教 (60345030)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥50,830,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥11,730,000); Fiscal Year 2010: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2009: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2008: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
• Keywords: 粘膜免役 / M細胞 / 上皮細胞 / 抗原取り込み / 共生細菌 / 粘膜免疫 / UEA-1 / フコシル化

Research Abstract

M (Microfold) cells and related subpopulation cells are known as specialized cells for antigen uptake. To reveal the extracellular regulating factors and the mechanisms for antigen uptake and their differentiation of M cells, we identified several M cell-specific genes, and investigated there functions on M cells. As a result, it was suggested that inducible M-like cells, which have been discovered by our group, were an intermediate cells between Peyer's patch M cells and intestinal epithelial cells. We succeeded in identifying GP2 and MLP as novel M cell-specific molecule. In the case of GP2, our collaborative efforts revealed that GP2 could capture some bacterial groups via FimH protein, and acted as a scaffold to uptake them efficiently. In addition, we established monoclonal anti-GP2 antibody, and verified that this antibody was applicable for effective vaccine antigen delivering to M cells.

Research Products

• [Journal Article] Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress (2011)
  • Author(s): Terahara K., et al.
  • Journal Title: Biochem Biophys Res Commun. 404 Pages: 822-828
  • Peer Reviewed
• [Journal Article] Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress. (2011)
  • Author(s): Terahara K, Nochi T, Yoshida M, Takahashi Y, Goto Y, Hatai H, Kurokawa S, Jang MH, Kweon MN, Domino SE, Hiroi T, Yuki Y, Tsunetsugu-Yokota Y, Kobayashi K, Kiyono H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 404 Pages: 822-828
  • Peer Reviewed
• [Journal Article] Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines. (2010)
  • Author(s): Nochi T., et al.
  • Journal Title: Nat Mater. 9 Pages: 572-578
  • Peer Reviewed
• [Journal Article] In vivo molecular imaging analysis of a nasal vaccine that induces protective immunity against botulism in nonhuman primates. (2010)
  • Author(s): Yuki Y., et al.
  • Journal Title: J Immunol. 185 Pages: 5436-5443
  • Peer Reviewed
• [Journal Article] Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine. (2010)
  • Author(s): Tokuhara D, et al.
  • Journal Title: Proc Natl Acad Sci USA. 107 Pages: 8794-8799
  • Peer Reviewed
• [Journal Article] Mucosal regulatory cells in the gastrointestinal tract and periodontium. (2010)
  • Author(s): Takahashi I., et al.
  • Journal Title: Periodontol. 54 Pages: 247-256
  • Peer Reviewed
• [Journal Article] Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis. (2010)
  • Author(s): Obata T., et al.
  • Journal Title: Proc Natl Acad Sci USA. 34 Pages: 768-774
  • Peer Reviewed
• [Journal Article] Antigen-sampling cells in the salmonid intestinal epithelium. (2010)
  • Author(s): Fuglem B., t al.
  • Journal Title: Dev Comp Immunol. 120 Pages: 2131-2143
  • Peer Reviewed
• [Journal Article] Aberrant interaction of the gut immune system with environmental factors in the development of food allergies. (2010)
  • Author(s): Kunisawa J, Kiyono H.
  • Journal Title: Curr Allergy Asthma Rep. 10 Pages: 215-221
  • Peer Reviewed
• [Journal Article] IL-15 triggers an antiapoptotic pathway in human intraepithelial lymphocytes that is a potential new target in celiac disease-associated inflammation and lymphomagenesis. (2010)
  • Author(s): Malamut G., et al.
  • Journal Title: J Clin Invest. 120 Pages: 2131-2143
  • Peer Reviewed
• [Journal Article] Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. (2010)
  • Author(s): Kayamuro H., et al., Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S.
  • Journal Title: J Virol. 84 Pages: 12703-12712
  • Peer Reviewed
• [Journal Article] Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines. (2010)
  • Author(s): Nochi T, Yuki Y, Takahashi H, Sawada S, Mejima M, Kohda T, Harada N, Kong IG, Sato A, Kataoka N, Tokuhara D, Kurokawa S, Takahashi Y, Tsukada H, Kozaki S, Akiyoshi K, Kiyono H.
  • Journal Title: Nat Mater. Volume: 9 Pages: 572-578
  • Peer Reviewed
• [Journal Article] In vivo molecular imaging analysis of a nasal vaccine that induces protective immunity against botulism in nonhuman primates. (2010)
  • Author(s): Yuki Y, Nochi T, Harada N, Katakai Y, Shibata H, Mejima M, Kohda T, Tokuhara D, Kurokawa S, Takahashi Y, Ono F, Kozaki S, Terao K, Tsukada H, Kiyono H.
  • Journal Title: JImmunol. Volume: 185 Pages: 5436-5443
  • Peer Reviewed
• [Journal Article] Secretory IgA-mediated protection against V.cholerae and heat-labileenterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine. (2010)
  • Author(s): Tokuhara D, Yuki Y, Nochi T, Kodama T, Mejima M, Kurokawa S, Takahashi Y, Nanno M, Nakanishi U, Takaiwa F, Honda T, Kiyono H.
  • Journal Title: Proc Natl Acad Sci USA. Volume: 107 Pages: 8794-8799
  • Peer Reviewed
• [Journal Article] Mucosal regulatory cells in the gastrointestinal tract and periodontium. (2010)
  • Author(s): Takahashi I, Fujihashi K, Kiyono H.
  • Journal Title: Periodontol Volume: 54 Pages: 247-256
  • Peer Reviewed
• [Journal Article] Indigenous opportunistic bacteria inhabit mammalian gut-associatedlymphoid tissues and share a mucosal antibody-mediated symbiosis. (2010)
  • Author(s): Obata T, Goto Y, Kunisawa J, Sato S, Sakamoto M, Setoyama H, Matsuki T, Nonaka K, Shibata N, Gohda M, Kagiyama Y, Nochi T, Yuki Y, Fukuyama Y, Mukai A, Shinzaki S, Fujihashi K, Sasakawa C, Iijima H, Goto M, Umesaki Y, Benno Y, Kiyono H.
  • Journal Title: Proc Natl Acad Sci USA. Volume: 107 Pages: 7419-7424
  • Peer Reviewed
• [Journal Article] Antigen-sampling cells in the salmonid intestinal epithelium. (2010)
  • Author(s): Fuglem B, Jirillo E, Bjerkas I, Kiyono H, Nochi T, Yuki Y, Raida M, Fischer U, Koppang EO.
  • Journal Title: Dev Comp Immunol. Volume: 34 Pages: 768-774
  • Peer Reviewed
• [Journal Article] Aberrant interaction of the gut immune system with environmentalfactors in the development of food allergies. (2010)
  • Author(s): Kunisawa J, Kiyono H.
  • Journal Title: Curr Allergy Asthma Rep. Volume: 10 Pages: 215-221
  • Peer Reviewed
• [Journal Article] IL-15 triggers an antiapoptotic pathway in human intraepithelial lymphocytes that is a potential new target in celiac disease-associated inflammation and lymphomagenesis. (2010)
  • Author(s): Malamut G, El Machhour R, Montcuquet N, Martin-Lanneree S, Dusanter-Fourt I, Verkarre V, Mention JJ, Rahmi G, Kiyono H, Butz EA, Brousse N, Cellier C, Cerf-Bensussan N, Meresse B.
  • Journal Title: J Clin Invest. Volume: 120 Pages: 2131-2143
  • Peer Reviewed
• [Journal Article] Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. (2010)
  • Author(s): Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S.
  • Journal Title: J Vjrol. Volume: 84 Pages: 12703-12712
  • Peer Reviewed
• [Journal Article] Antigen-sampling cells in the salmonid intestinal epithelium. (2010)
  • Author(s): Fuglem, B., et al.
  • Journal Title: Dev Comp Immunol. 34 Pages: 253-257
  • Peer Reviewed
• [Journal Article] Mucosal immunosenescence : New developments and vaccines to contr-ol infectious diseases. (2009)
  • Author(s): Fujihashi, K., Kiyono, H.
  • Journal Title: Trends Immunol. 30 Pages: 334-343
  • Peer Reviewed
• [Journal Article] Expression of newly identified secretory CEACAM1 (a) isoforms in the intestional epithelium. (2009)
  • Author(s): Terahara, K., et al.
  • Journal Title: Biochem Biophys Res Commun. 383 Pages: 340-346
  • Peer Reviewed
• [Journal Article] Uptake through glycoprotein 2 of FimH (+) bacteria by M cells initiates mucosal immune response. (2009)
  • Author(s): Hase, K., et al.
  • Journal Title: Nature 462 Pages: 226-230
  • Peer Reviewed
• [Journal Article] RANKL is necessary and sufficient to initiate development of antigen-sampling M cells in the intestinal epithelium. (2009)
  • Author(s): Knoop, K.A., et al.
  • Journal Title: J.Immunol 183 Pages: 5738-5747
  • Peer Reviewed
• [Journal Article] Localization of fatty acid binding protein of epidermal type common to dendritic cells and presumptive macrophages in Peyer's patches and epithelial M cells of mouse intestine. (2009)
  • Author(s): Suzuki, R., et al.
  • Journal Title: Histochem Cell Biol. 132 Pages: 577-584
  • Peer Reviewed
• [Journal Article] Id2-, RORγt-, and LTβR-independent lymphoid organogenesis in ocular immunity. (2009)
  • Author(s): Nagatake, T., et al.
  • Journal Title: J.Exp.Med. 206 Pages: 2351-2364
  • Peer Reviewed
• [Journal Article] New Horizon of mucosal immunity and vaccine. (2009)
  • Author(s): Takahashi, T., et al.
  • Journal Title: Curr.Opin.Immunol. 21 Pages: 352-358
  • Peer Reviewed
• [Journal Article] A Rice-based oral cholera vaccine induces macaque-specific systemic neutralizing Abs but does not influence pre-existing intestinal immunity. (2009)
  • Author(s): Nochi, T., et al.
  • Journal Title: J.Immunol. 183 Pages: 6538-6544
  • Peer Reviewed
• [Journal Article] Mucosal vaccines : novel advances in technology and delivery. (2009)
  • Author(s): Yuki, Y., Kiyono, H.
  • Journal Title: Expert Rev Vaccines. 8 Pages: 1083-1097
  • Peer Reviewed
• [Journal Article] Comprehensive gene expression analysis among peyer's patch M cells, villous M cells and intestinal epithelial cells by DNA microarray analysis. (2008)
  • Author(s): Terahara, T., Igarashi, O., Yoshid a, M., Nochi, T., Kurokawa, S.,Takayama, N., Yuki, Y., Low, A W. and Kiyono, H.
  • Journal Title: J. Immunol. 180 Pages: 7840-7846
  • Peer Reviewed
• [Journal Article] Regulation of humoral and cellular gut immunity by lamina propria den dritic cells expressing Toll-like receptor 5. (2008)
  • Author(s): Uematsu,S., Fujimoto, K., Jang, MH., Yang, BG., Jung, YJ., Nishi yama, M., Sato, S., Tsujimura, T. Yamamoto, M., Yokota, Y., Kiyno, H., Miyasaka, M., Ishii, KJ., Akira, S.
  • Journal Title: Nat Immunol 9 Pages: 769-776
  • Peer Reviewed
• [Journal Article] Cutting edge: Langerin+ dendritic cells in the mesenteric lymph node set the stage for skin and gut immune system cross-talk (2008)
  • Author(s): Chang, SY., Cha, HR., Igarashi, O., Rennert, PD., Kissenpfennig, A., Malissen, B., Nanrto, M., Kiy. ono, H., and Kweon, MN.
  • Journal Title: J. Imrnuriol. 180 Pages: 4361-4365
  • Peer Reviewed
• [Journal Article] Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer's patches for intestinal IgA responses. (2008)
  • Author(s): Gohda, M., Kunisawa, J., Miura, F., Kagiyama, Y., Kurashima, Y. , Higuchi, M., Ishikawa, I., Ogahara, I. and Kiyono, H.
  • Journal Title: J. Immunol. 180 Pages: 5335-5343
  • Peer Reviewed
• [Journal Article] Colonic patches direct the cross-talk between systemic compartments and large intestine independently of innate immunity. (2008)
  • Author(s): Sun-Young Chang, Hye-Ran Cha, Uematsu, S., Akira, S., Igaras hi, O., Kiyono, H.,and Mi-Na Kweon
  • Journal Title: J. Immunol 180 Pages: 1609-1618
  • Peer Reviewed
• [Journal Article] Sphingosine 1-phosphate-dependent trafficking of peritoneal B cells requires functional NFkB-inducing kinase in stromal cells. (2008)
  • Author(s): Kunisawa, J., Gohda, M., Kurashima, Y., Ishikawa, I., Higuchi, M . and Kiyono, H.
  • Journal Title: Blood. 111 Pages: 4646-4652
  • Peer Reviewed
• [Journal Article] Peyer's patches are required for intestinal IgA responses to Salmonella. (2008)
  • Author(s): Hashizume, T., Togawa, A., Nochi, T., Igarashi, O., Kweon, M. N. , Kiyono, H. and Yamamoto, M.
  • Journal Title: Infect Immun. 76 Pages: 927-934
  • Peer Reviewed
• [Journal Article] Nasal Vaccination with the 40-Kilodalton outer membrane protein of Porphyromonas gingivalis and a nontoxic chimeric enterotoxin adjuvant induces lomg-term protective immunity with reduced levels of immunoglobulin E antibodies. (2008)
  • Author(s): Momoi, F., Hashizume, T., Kurita-Ochiai, T., Yuki, Y., Kiyono, H ., and Yamamoto, M.
  • Journal Title: Infect Immun. 76 Pages: 7840-7846
  • Peer Reviewed
• [Journal Article] Tonic B cell activation by radioprotective105/MD-1 promotes disease progression in MRL/lpr mice. (2008)
  • Author(s): Kobayashi T, Takahashi K, Nagai Y, Shibata T, Otani M, Izui S, Akira S, Gotoh Y, Kiyorto H, and Miyake K
  • Journal Title: Int Immunol. 20 Pages: 881-891
  • Peer Reviewed
• [Journal Article] Immunological commonalities and distinctions between airway and digestive immunity. (2008)
  • Author(s): Kunisawa, J., Nochi, T. and Kiyono, H.
  • Journal Title: Trends Immunol. 29 Pages: 505-513
  • Peer Reviewed
• [Presentation] Chaperon-Like Antigen Presentaion by Nanogel-Based Vaccine at Nasal Epithelium. (2011)
  • Author(s): KIYONO H.
  • Organizer: Avison Biomedical Symposium 2011
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-02-25
• [Presentation] Chaperon-Like Antigen Presentaion by Nanogel-Based Vaccine at Nasal Epithelium (2011)
  • Author(s): HIROSHI KIYONO
  • Organizer: Avison Biomedical Symposium 2011
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-02-25
• [Presentation] New Prospects on the Aerodigestive Immunity for Mucosal Vaccine Development. (2010)
  • Author(s): KIYONO H.
  • Organizer: Prato Conference on the Pathogenesis of Bacterial Diseasesof Animals.
  • Place of Presentation: Prato, Italy
  • Year and Date: 2010-10-09
• [Presentation] New Prospects on the Aerodigestive Immunity for Mucosal Vactine Development (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: Prato Conference on the Pathogenesis of Bacterial Diseasesof Animals
  • Place of Presentation: Prato, Italy
  • Year and Date: 2010-10-09
• [Presentation] Mucosal Nanogel-based Chaperon Vaccine for the induction of protective immunity. (2010)
  • Author(s): KIYONO H.
  • Organizer: 4^<th> Vaccine and ISV Annual Global Congress.
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2010-10-04
• [Presentation] Mucosal Nanogel-based Chaperon Vaccine for the induction of protective immunity (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: 4^<th> Vaccine and ISV Annual Global Congres
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2010-10-04
• [Presentation] Intra-Tissue habitation of commensal bacteria in Peyer's patches for gut immunity. (2010)
  • Author(s): KIYONO H.
  • Organizer: 8^<th> German-Japanease Symposium, Regulation of immune response and diseases.
  • Place of Presentation: Cuxhahen, Germany
  • Year and Date: 2010-09-27
• [Presentation] Intra-Tissue habitation of commensal bacteria in Peyer's patches for gut immunity (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: 8^<th> German-Japanease Symposium, Regulation of immune response and diseases
  • Place of Presentation: Cuxhahen, Germany
  • Year and Date: 2010-09-27
• [Presentation] Intra-tissue habitation of intestinal opportunistic bacteria in mammalian gut-associated lymphoid tissues for the creation of a mucosal antibody-mediated symbiosis. (2010)
  • Author(s): KIYONO H.
  • Organizer: 40^<th> Annual Meeting DGFI German Society for Immunology.
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 2010-09-26
• [Presentation] Intra-tissue habitation of intestinal opportunistic bacteria in mammalian gut-associated lymphoid tissues for the creation of a mucosal antibody-mediated symiosis (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: 40^<th> Annual Meeting DGFI German Society for Immunology
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 2010-09-26
• [Presentation] Mucosal chaperoning nanogel vaccine for the induction of protective immunity. (2010)
  • Author(s): KIYONO H.
  • Organizer: The 10^<th> Awaji International Forum on Infection and Immunity (United states-Japan Cooperative Medical Science Program 23rd Joint Meeting of the AIDS Panels.
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2010-09-08
• [Presentation] Mucosal chaperoning nanogel vaccine for the induction of protective immunity (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: The 10^<th> Awaji Inteertional Forum on Infection and Immunity (United states-Japan Cooperative Medical Science Program 23^<rd> Joint Meeting of the AIDS Panels
  • Place of Presentation: Awaji, 淡路夢舞台国際会議場
  • Year and Date: 2010-09-08
• [Presentation] Role of Intestinal Glycosylation for the Mucosal Immne System and Mucosal Vaccine Development. (2010)
  • Author(s): KIYONO H.
  • Organizer: 1^<st> Asia Pacific Workshop on Neurovirology Seoul 2010
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-07-16
• [Presentation] Role of Intestinal Glycosylation for the Mucosal Immne System and Mucosal Vaccine Development (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: 1^<st> Asia Pacific Workshop on Neurovirology Seoul 2010
  • Place of Presentation: Seoul, Korea.
  • Year and Date: 2010-07-16
• [Presentation] Craniofacial Mucosal Immune Sysem : Uniqueness of lymphoid Organogenesis and Immunological Function for NALT and TALT. (2010)
  • Author(s): KIYONO H.
  • Organizer: The 7^<th> Intenational Symposium on Tonsils and Mucosal Barriers of the Upper Airways
  • Place of Presentation: Asahikawa., Hokkaido
  • Year and Date: 2010-07-08
• [Presentation] Craniofacial Mucosal Immune Sysem : Uniqueness of lymphoid Organogenesis and Immunological Function for NALT and TALT (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: The 7^<th> Intenational Symposium on Tonsilsand Mucosal Barriers of the Upper Airways
  • Place of Presentation: Asahikawa Grand Hotel
  • Year and Date: 2010-07-08
• [Presentation] Mucosal Immunity in the Control of Infectious Diseases. (2010)
  • Author(s): KIYONO H
  • Organizer: Pasteur-Areva Course Anti-Viral Imjmunity
  • Place of Presentation: Shanghai., China
  • Year and Date: 2010-05-28
• [Presentation] Mucosal Immunity in the Control of Infectious Diseases (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: Pasteur-Areva Course Anti-Viral Imjmunity
  • Place of Presentation: Shanghai, China
  • Year and Date: 2010-05-28
• [Presentation] Basic principals of immune responces: innate versus adaptive immunity. (2010)
  • Author(s): KIYONO H.
  • Organizer: The 10^<th> International Advanced Course on Vaccinology in Asia Pacific Region.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-05-10
• [Presentation] Basic principals of immune responces : innate versus adaptive immunity (2010)
  • Author(s): HIROSHI KIYONO
  • Organizer: The 10^<th> International Advanced Course on Vaccinology in Asia Pacific Region
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-05-10
• [Presentation] New Prospect for Mucosal Vaccine Development : MucoRiceTM for Refrigeration-and Needle/Shiringe-free vaccine. (2010)
  • Author(s): 清野宏
  • Organizer: The 2nd International Symposium of Innovative Bioproduction Kobe (iBioK)
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-01-22
• [Presentation] Novel Strategy for Mucosal Vaccine Development : MucoRice and MucoNanogel (2010)
  • Author(s): 清野宏
  • Organizer: Adaptive and Innate Immune Resposes to Neglected Tropical Disease Conference
  • Place of Presentation: SanDiego, USA
  • Year and Date: 2010-01-10
• [Presentation] MucoRice : New Horizon for the Development of Oral Vaccine (2009)
  • Author(s): 清野宏
  • Organizer: 12th Asia Pacific-International Molecular Biology Network (A-IMBN) Symposium
  • Place of Presentation: Penang, Malaysia
  • Year and Date: 2009-10-27
• [Presentation] New Prospect for Vaccine Development : MucoRice and MucoNanogel (2009)
  • Author(s): 清野宏
  • Organizer: 3rd Vaccine Global Congress
  • Place of Presentation: Singapore
  • Year and Date: 2009-10-04
• [Presentation] The Craniofacial and Mucosal Immune System (2009)
  • Author(s): 清野宏
  • Organizer: Japanese Journal of Rhinology
  • Place of Presentation: 島根,日本
  • Year and Date: 2009-10-03
• [Presentation] Mucosal Harmony for Immunity and Tolerance in Vaccine Development (2009)
  • Author(s): 清野宏
  • Organizer: 2nd European Congress of Immunology
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2009-09-14
• [Presentation] 粘膜免疫による腸内細菌共生機構の構築 (2009)
  • Author(s): 清野宏
  • Organizer: 第63回日本細菌学会 東北支部総会
  • Place of Presentation: 盛岡,日本
  • Year and Date: 2009-08-20
• [Presentation] Rice-Based Vaccine : New Generation of Mucosal Vacine (2009)
  • Author(s): 清野宏
  • Organizer: 14th International Congress of Mucosal Immunology
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-07-08
• [Presentation] 粘膜免疫による腸内細菌共生の構築 (2009)
  • Author(s): 清野宏
  • Organizer: 第91回日本消化器病学会 中国支部例会
  • Place of Presentation: 岡山,日本
  • Year and Date: 2009-06-13
• [Presentation] Mucosal Decision of Immunity and Tolerance for Creation of Symbiosis (2009)
  • Author(s): 清野宏
  • Organizer: The 6th Extraordinary International Symposium on Recent Advances in Otitis Media
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-05-06
• [Presentation] Mucosal Immunity : A Driving Force for the Development of New Generation of Oral Vaccine (2009)
  • Author(s): 清野宏
  • Organizer: The 13th International Conference on Emerging Infection Dieseases of the Pacific Rum : Focus on Enteric Diseases.
  • Place of Presentation: Kolkata, India.
  • Year and Date: 2009-04-06
• [Presentation] Session1"Id2, RORgt, and LTbR independent initiation of TALT genesis in ocular immunity."and Session2"Indigenous opportunistic bacteria inhabit mammalian gut associated lymphoid tissues for gut immunity." (2009)
  • Author(s): 清野宏
  • Organizer: Mucosal immunology Workshop at Trudeau Institute
  • Place of Presentation: New York, USA
• [Book] 細胞工学(粘膜免疫システム) (2011)
  • Author(s): 清野宏
  • Publisher: (株)秀潤社
• [Book] 臨床粘膜免疫学 (2010)
  • Author(s): 清野宏
  • Total Pages: 722
  • Publisher: (株)シナジー
• [Book] 臨床粘膜免疫学 (2010)
  • Author(s): 清野宏
  • Total Pages: 722
  • Publisher: 株式会社シナジー
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 炎症性腸疾患の検査方法及び検査用キット (2010)
  • Inventor(s): 清野宏, 佐藤慎太郎, 小幡高士, 飯島英樹
  • Industrial Property Rights Holder: 清野宏, 佐藤慎太郎, 小幡高士, 飯島英樹
  • Industrial Property Number: 2010-072790
  • Filing Date: 2010-03-26