The Mechanism Underlying the Formation of Activity-Dependent Thalamocortical Projection

• Project/Area Number: 20300110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Osaka University
• Principal Investigator: YAMAMOTO Nobuhiko Osaka University, 大学院・生命機能研究科, 教授 (00191429)
• Co-Investigator(Kenkyū-buntansha): SUGO Noriyuki 大阪大学, 大学院・生命機能研究科, 助教 (20372625)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 軸索分岐 / 軸索誘導 / 神経活動 / 層特異性 / 視床 / 大脳 / HDAC9 / 遺伝子発現調節 / c-fos / 樹状突起 / 核細胞質移動 / 軸索 / 同期性 / Kir2.1 / 軸索分枝 / RhoA / 神経回路

Research Abstract

Neural activity is well known to play an important role in neural circuit formation. However, the mechanism is largely unknown. We studied the role of neural activity in axonal branch formation, focusing on the thalamocortical projection in the mammalian brain. The result shows that both activities of growing thalamic axons (presynaptic) and their target cortical neurons (postsynaptic) promote axon branching. Evidence also suggests the underlying molecular mechanisms.

Research Products

• [Journal Article] A molecular mechanism that regulates medially oriented axonal growth of upper layer neurons in the developing neocortex. (2011)
  • Author(s): Zhao H, Maruyama T, Hattori Y, Sugo N, Takamatsu H, Kumanogoh A, Shirasaki R, Yamamoto N
  • Journal Title: J Comp Neurol 519 Pages: 834-848
  • Peer Reviewed
• [Journal Article] Laminar and Areal Expression of Unc5d and Its Role in Cortical Cell Survival (2011)
  • Author(s): Takemoto M, Hattori Y, Zhao H, Sato H, Tamada A, Sasaki S, Nakajima K, Yamamoto N
  • Journal Title: Cerebral Cortex Volume: (Epub)
  • Peer Reviewed
• [Journal Article] Role of pre- and postsynaptic activity in thalamocortical axon branching. (2010)
  • Author(s): Yamada A, Uesaka N, Hayano Y, Tabata T, Kano M, Yamamoto N
  • Journal Title: Proc Natl Acad Sci USA 107 Pages: 7562-7567
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons. (2010)
  • Author(s): Sugo N, Oshiro H, Takemura M, Kobayashi T, Kohno Y, Uesaka N, Song WJ, Yamamoto N
  • Journal Title: Eur J Neurosci 31 Pages: 1521-1532
  • Peer Reviewed
• [Journal Article] Role of pre- and postsynaptic activity in thalamocortical axon branching. (2010)
  • Author(s): Yamada A, Uesaka N, Hayano Y, Tabata T, Kano M, Yamamoto N
  • Journal Title: Proc Natl Acad Sci of USA Volume: 107 Pages: 7562-7567
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons. (2010)
  • Author(s): Sugo N, Oshiro H, Takemura M, Kobayashi T, Kohno Y, Uesaka N, Song W-J, Yamamoto N
  • Journal Title: European Journal of Neuroscience Volume: 31 Pages: 1521-1532
  • Peer Reviewed
• [Journal Article] Role of pre-and Postsynaptic activity in thalamocortical axon branching. (2010)
  • Author(s): Yamada A, Uesaka N, Hayano Y, tabata T, Kano M, Yamamoto N
  • Journal Title: Proc Natl Acad Sci of USA (印刷中)
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons. (2010)
  • Author(s): Sugo N, Oshiro H, Takemura M, Kobayashi T, Kohno Y, Uesaka N, Song W-J, Yamamoto N
  • Journal Title: Eur J Neurosci (印刷中)
  • Peer Reviewed
• [Journal Article] FGF8 signaling regulates growth of midbrain dopaminergic axons by inducing semaphorin 3F. (2009)
  • Author(s): Yamauchi K, Mizushima S, Tamada A, Yamamoto N, Takashima S, Murakami F
  • Journal Title: J Neurosci 29 Pages: 4044-4055
  • Peer Reviewed
• [Journal Article] Role of RhoA in activity-dependent cortical axon branching. (2008)
  • Author(s): Ohnami S, Endo M, Hirai S, Uesaka N, Hatanaka Y, Yamashita T, Yamamoto N
  • Journal Title: J Neurosci 28 Pages: 9117-9121
  • Peer Reviewed
• [Journal Article] Cooperative activity of multiple upper layer proteins for thalamocortical axon growth. (2008)
  • Author(s): Maruyama T, Matsuura M, Suzuki K, Yamamoto N
  • Journal Title: Dev Neurobiol 68 Pages: 317-331
  • Peer Reviewed
• [Journal Article] Single cell electroporation method for axon tracing in cultured slices. (2008)
  • Author(s): Uesaka N, Nishiwaki M, Yamamoto N
  • Journal Title: Dev Growth Differ 50 Pages: 475-477
  • NAID: 10021097209
• [Journal Article] Activity-dependent thalamocortical axon branching. (2008)
  • Author(s): Hayano Y, Yamamoto N
  • Journal Title: Neuroscientist 14 Pages: 359-368
• [Journal Article] Role of RhoA in activity-dependent cortical axon branching (2008)
  • Author(s): Ohnami, et al.
  • Journal Title: J. Neurosci. 28 Pages: 9117-9121
  • Peer Reviewed
• [Journal Article] Cooperative activity of multiple upper layer proteins forthalamocortical axon growth (2008)
  • Author(s): Maruyama, et al.
  • Journal Title: Dev. Neurobiol. 68 Pages: 317-331
  • Peer Reviewed
• [Journal Article] Single cell electroporation method. for axon tracing in cultured slices. (2008)
  • Author(s): Uesaka, et al.
  • Journal Title: Dev. Growth. Differ 50 Pages: 475-477
• [Presentation] Activity-dependent thalamocortical axon branching. (2011)
  • Author(s): Yamamoto N.
  • Organizer: Japan-Australia-New Zealand "Building a Functional Brain" Symposium
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2011-01-29
• [Presentation] Activity-dependent thalamocortical axon branching. (2011)
  • Author(s): Yamamoto N.
  • Organizer: Development and Plasticity of Thalamocortical Systems
  • Place of Presentation: Arolla, Switzerland
• [Presentation] Netrin-4 promotes thalamocortical axon branching in a lamina-specific and activity-dependent fashion (2010)
  • Author(s): Hayano Y, Takemoto M, Maeda Y, Kitada K, Yamamoto, N
  • Organizer: Axon Guidance, Synapic Plasticity & Regeneration.
  • Place of Presentation: Cold Spring Harbor, New York
• [Presentation] Thalamus-derived molecules promote survival and dendritic growth of developing cortical neurons Abstract (2010)
  • Author(s): Sato H, Tatara E, Yamamoto Y, Fukutani Y, Takemoto M, Yamamoto N
  • Organizer: Axon Guidance, Synapic Plasticity & Regeneration
  • Place of Presentation: Cold Spring Harbor, New York
• [Presentation] Molecular Mechanisms of Oriented Axon Growth of Cortical Projection Neurons. (2009)
  • Author(s): Yamamoto N.
  • Organizer: Construction and Reconstruction of the Brain
  • Place of Presentation: Awaji, Japan
• [Presentation] The role of pre-and post-synaptic cell activity in thalamocortical axon branching. (2008)
  • Author(s): Yamamoto N, Yamada A, Hayano Y, Uesaka N
  • Organizer: Cortical Development
  • Place of Presentation: Chania, Greece
  • Year and Date: 2008-05-22
• [Presentation] BNDF regulates activity-dependent axonal branching of cortical neurons. (2008)
  • Author(s): Nishiwaki M, Komatsu Y, Yamamoto N
  • Organizer: Axon Guidence, Synaptogenesis & Neural Plasticity
  • Place of Presentation: Cold Spring Harbor, New York
• [Presentation] Activity-dependent nucleocytoplasmic translocation of TORC1 regulates gene expression and dendritic growth in developing cortical neurons. (2008)
  • Author(s): Sugo N, Kobayashi T, Yamamoto N
  • Organizer: Axon Guidance, Synaptogenesis & Neural Plasticity.
  • Place of Presentation: Cold Spring Harbor, New York
• [Presentation] Pre-and post synaptic cell firing history regulates branch formation in thalamocortical. (2008)
  • Author(s): Yamamoto N, Hayashi A, Yamada A, Uesaka N, Hayano Y
  • Organizer: Axon Guidence, Synaptogenesis & Neural Plasticity
  • Place of Presentation: Cold Spring Harbor, New York
• [Presentation] A molecular mechanism that regulates callosal axon growth. (2008)
  • Author(s): Zhao H, Maruyama T, Shirasaki R, Yamamoto N
  • Organizer: Axon Guidence, Synaptogenesis & Neural Plasticity.
  • Place of Presentation: Cold Spring Harbor, New York
• [Presentation] LAMINA-and area-specific expression of UNC5H4 and its role in cortical development. (2008)
  • Author(s): Takemoto M, Hattori Y, Yamamoto N
  • Organizer: Cortical Development.
  • Place of Presentation: Chania, Greece
• [Book] Electroporation and Sonoporation in Developmental Biology((Nakamura H, ed))(Single cell electroporation method for mammalian CNS neurons in organorypic slice cultures.) (2009)
  • Author(s): Uesaka N, Hayano Y, Yamada A, Yamamoto N
  • Publisher: Springer
• [Remarks] ホームページURL
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/~neurobiol/