| Project/Area Number |
20300147
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
KIKKAWA Yoshiaki Tokyo University of Agriculture, 生物産業学部, 准教授 (20280787)
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| Co-Investigator(Kenkyū-buntansha) |
SHITARA Hiroshi (財)東京都医学研究機構, 東京都臨床医学総合研究所, 研究員 (90321885)
NOGUCHI Yoshihiro 東京医科歯科大学, 医学部付属病院, 講師 (50282752)
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| Research Collaborator |
STEVE Brown 英国MRC, Mammalian Genetics Unit, 教授
奥村 和弘 東京農業大学, 大学院, 博士後期課程
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 育種遺伝 / 分子間相互作用 / 難聴 / マウス / 有毛細胞 / Stereocilia / 難聴発症機構 / ヒト難聴モデルマウス / ミオシンVI / カドヘリン23 / 加齢性難聴 / 修飾遺伝子 / ゲルソリン / DBA / 2J / 4.1蛋白質 / Sans / Whirlin / Myosin VI / マウス亜種間交配 |
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| Research Abstract |
To establish new mouse models for deafness and identify molecular mechanism of hearing impairment, we carried out positional cloning approach, expression analysis of the inner ear hair cells and protein-protein interaction studies among the deafness associated-proteins. 1) We identified that responsible gene of two new spontaneous mutants is myosin VI. 2) 4.1B and gelsolin may be members of the myosin XV-whirlin complex that is important for stereocilia maturation. 3) Phenotypic data of double homozygotes between Sans and Whrlin mutants suggest that the function of SANS interacts with WHRLIN and the interaction play regulation of planar cell polarity in stereocilia of the hair cells.
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