Three dimensionally layered spheroid array for tissue organization
| Project/Area Number |
20300170
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
OTSUKA Hidenori Tokyo University of Science, 理学部, 准教授 (00344193)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHI Kazuto 東京大学, 医学部付属病院, 准教授 (30344451)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | 高分子ゲル / マイクロパターニング / スフェロイド / 軟骨再生 / 微細加工 / 軟骨 / 再生医療 / 組織工学 |
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| Research Abstract |
For formation of tissue elements, spheroid is successfully constructed with a diameter of 100 or 200 ・m on a micropatterned substrate coated with polyethylene glycol (PEG). The PEGylated surface as a cytophobic region was regulated by controlling the polymer density through photolithography. This spheroid formation induces epitaxially growing chondrocyte spheroids perpendicular to the surface with long-term viability. Such interesting observation was resulted from the spontaneous production of extracellular matrix (ECM) protein and concomitant formation of native cartilage-like tissue. This approach aims to overcome the disadvantages associated with the hanging drop and suspension culture methods by providing control over the size, shape, and other features of the cellular assembly in a scalable manner. It is envisioned that with the integration of presented technologies with biomaterials such as photocrosslinkable gels and microfluidics, more complex tissue sections for therapeutic applications can be fabricated.
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Report
(4 results)
Research Products
(201 results)
