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Analysis of the interaction between bacterial cells and hydrocarbons in oil-degrading bacteria and its application to bioremediation.

Research Project

Project/Area Number 20310047
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionNihon University

Principal Investigator

OGIHARA Jun (2010)  日本大学, 生物資源科学部, 講師 (50256830)

中嶋 睦安 (2008-2009)  Nihon University, 生物資源科学部, 教授 (10059660)

Co-Investigator(Kenkyū-buntansha) SUNAIRI Michio  日本大学, 生物資源科学部, 教授 (80196906)
IWABUCHI Noriyuki  日本大学, 生物資源科学部, 講師 (90328708)
FURUKAWA Sohichi  日本大学, 生物資源科学部, 講師 (40339289)
ANZAI Hiroshi  日本大学, 短期大学部・生物資源学科, 教授 (70168029)
荻原 淳  日本大学, 生物資源科学部, 講師 (50256830)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
KeywordsRhodococcus / グリーンバイオテクノロジー / バイオリメディエーション / プロテオーム解析 / 土壌汚染 / 物理化学的相互作用 / ホワイトバイオテクノロジー / Mg
Research Abstract

Rhodococcus erythropolis PR4 is an alkane-degrading bacterium, which grows well in media containing high concentrations of alkanes with translocating to them. These properties give the organism potential in the bioremediation of various environments contaminated by alkanes. In this study, we report the translocation of R. erythropolis PR4 from an aqueous phase to an alkane phase during growth in a two phase culture medium to understand the translocation of PR4 to the alkanes.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (8 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Bacillus trypoxylicola sp. nov., a xylanase-producing alkaliphilic bacterium isolated from larval gut of Trypoxylus dichotomus, the Japanese horned beetle2010

    • Author(s)
      T. Aizawa, N. Iwabuchi, M. Sunairi, M. Nalajima, et al
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 60 Pages: 61-66

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bacillus trypoxylicola sp.nov., a xylanase-producing alkaliphilic bacterium isolated from larval gut of Trypoxylus dichotomus, the Japanese horned beetle2010

    • Author(s)
      Aizawa, Iwabuchi Sunairi, et al
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 60 Pages: 61-66

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Removing Staphylococcus aureus and Escherichia coli biofilms on stainless steel by cleaning-in-place (CIP) cleaning agents2010

    • Author(s)
      Furukawa, et al
    • Journal Title

      Food Control

      Volume: 21 Pages: 669-672

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morinaga, Sugar fatty acid esters inhibit biofilm formation by food-borne pathogenic bacteria2010

    • Author(s)
      Furukawa, et al
    • Journal Title

      Journal of Food Microbiology

      Volume: 138 Pages: 176-180

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生物膜成分による石油汚染海洋の浄化-細胞外多糖の性質と機能およびそれを利用した微生物制御技術-2010

    • Author(s)
      岩淵範之
    • Journal Title

      水環境学会誌

      Volume: 33(4) Pages: 359-364

    • NAID

      10026288978

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Bacillus trypoxylicola sp.nov., a xylanase-producing alkaliphilic bacterium isolated from larval gut of Trypoxylus dichotomus, the Japanese horned beetle2010

    • Author(s)
      T.Aizawa, N.Iwabuchi, M.Sunairi, M.Nalajima, et al.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 60

      Pages: 61-66

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Interfacial Tension in the Translocation of Rhodococcus erythropolis during Growth in a Two-Phase Culture System2009

    • Author(s)
      N. Iwabuchi, M. Sunairi, M. Nakajima, et al
    • Journal Title

      Environmental Science & Technology

      Volume: 43 Pages: 8290-8294

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biofilm formation by Escherichia coli in hypertonic sucrose media2009

    • Author(s)
      T. Kawarai, S. Furukawa, et al
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 107 Pages: 630-635

    • NAID

      110007330982

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] LL-Z1272αepoxide, a precursor of ascochlorin produced by a mutant of Ascochyta viciae2009

    • Author(s)
      K. Hosono, J. Ogihara, et al
    • Journal Title

      Journal of Antibiotics

      Volume: 62 Pages: 571-574

    • NAID

      10030636512

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Interfacial Tension in the Translocation of Rhodococcus erythropolis during Growth in a Two-Phase Culture System2009

    • Author(s)
      N.Iwabuchi, M.Sunairi, M.Nakajima, et al.
    • Journal Title

      Environmental Science & Technology 43

      Pages: 8290-8294

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biofilm formation by Escherichia coli in hypertonic sucrose media2009

    • Author(s)
      T.Kawarai, S.Furukawa, et al.
    • Journal Title

      Journal of Bioscience and Bioengineering 107

      Pages: 630-635

    • NAID

      110007330982

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LL-Z1272α epoxide, a precursor of ascochlorin produced by a mutant of Ascochyta viciae2009

    • Author(s)
      K.Hosono, J.Ogihara, et al.
    • Journal Title

      Journal of Antibiotics (In press)

    • NAID

      10030636512

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Rhodococcus erythropolis PR4株の培地/アルカン二相培養系における各種プロテオーム解析2010

    • Author(s)
      瀧原、岩淵、荻原、砂人, ら
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海洋性多環芳香族炭化水素(PAHs)分解菌Cycloclasticusの培養制御技術の開発2010

    • Author(s)
      荻原、砂入、岩淵, ら
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海洋性多環芳香族炭化水素(PAHs)分解菌Cycloclasticusのプロテオーム解析2010

    • Author(s)
      荻原、砂入、岩淵, ら
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cycloclasticus属細菌の生育と多環芳香族炭化水素(PAHs)の分解に対する鉄の影響2010

    • Author(s)
      荻原、砂入、岩淵, ら
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酵母と乳酸菌の複合バイオフィルム形成2009

    • Author(s)
      古川壮
    • Organizer
      BioJapan2009
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-10-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 油-微生物の相互作用における細胞外多糖(EPS)の影響2009

    • Author(s)
      岩淵範之
    • Organizer
      Bacterial Adherence & Biofilm 第23回学術集会
    • Place of Presentation
      第一三共
    • Year and Date
      2009-07-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Rhodococcus erythropolis PR4株の培地/アルカン二層培養系におけるGroEL2の影響2009

    • Author(s)
      瀧原速仁, 岩淵範之, 砂入道夫, 中嶋睦安
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 油-微生物の相互作用におけるバイオフィルムの役割を考える2009

    • Author(s)
      岩淵 範之
    • Organizer
      日本農芸化学会シンポジウム
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 形質転換されたロドコッカス(Rhodococcus)属細菌、及び、これを用いた炭化水素の処理方法2009

    • Inventor(s)
      岩淵範之、中嶋睦安、砂入道夫, 他
    • Industrial Property Rights Holder
      日本大学
    • Filing Date
      2009-09-09
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ロドコッカス(Rhodococcus)属細菌を用いた代謝方法2009

    • Inventor(s)
      岩淵範之、中嶋睦安、砂入道夫, 他
    • Industrial Property Rights Holder
      日本大学
    • Filing Date
      2009-08-24
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 形質転換されたロドコッカス(Rhodococcus)属細菌、及び、これを用いた炭化水素の処理方法2009

    • Inventor(s)
      岩淵範之, 中嶋睦安, 砂入道夫, 他
    • Industrial Property Rights Holder
      日本大学
    • Filing Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] ロドコッカス(Rhodococcus)属細菌を用いた代謝方法2009

    • Inventor(s)
      岩淵範之, 中嶋睦安, 砂入道夫, 他
    • Industrial Property Rights Holder
      日本大学
    • Filing Date
      2009-08-24
    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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