スラブ光導波路分光法を用いたタンパク質の電子移動反応のその場測定

• Project/Area Number: 20310065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: MATSUDA Naoki National Institute of Advanced Industrial Science and Technology, 生産計測技術研究センター, 研究チーム長 (10344219)
• Co-Investigator(Kenkyū-buntansha): 二又 政之 国立大学法人埼玉大学, 大学院・理工学研究科, 教授 (20344161)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2008: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: スラブ光導波路分光法 / タンパク質 / 電子移動反応 / 固液界面 / 紫外可視吸収スペクトル / 吸着過程 / ITO電極 / 速度論 / スラブ光導波路

Research Abstract

Time-resolved electrochemical slab optical waveguide spectroscopy utilizing ITO electrode with 1 msec time resolution was developed. The absorption spectral change from cytochrome c adsorbed on ITO electrode due to pulse potential step between oxidized and reduced form was observed every 2 msec. The time constant of electron transfer (ET) reaction between cytochrome c and ITO electrode was estimated to be about 10 msec from the fitting of the time- resolved intensity change in SOWG absorption spectra. Finally as a result of this study, ET rate constant (k) was evaluated to be 100 [s^<-1>].

Research Products

• [Journal Article] In situ observation of reduction behavior of cytochrome c adsorbed on glass surface by slab optical waveguide spectroscopy (2011)
  • Author(s): Naoki Matsuda, Hirotaka Okabe, Masaki Fujii, Masayoshi Matsui, Yusuke Ayato, Akiko Takatsu, Kenji Kato
  • Journal Title: IEICE Trans. Electron. E94-C Pages: 170-175
  • NAID: 10028231537
• [Journal Article] Elucidation of Electrostatic Interaction between Cationic Dyes and Ag Nanoparticles Generating Enrormous SERS Enhancement in Solution (2011)
  • Author(s): Masayuki Futamata, Yingying Yu,, Toru Yajima.
  • Journal Title: J.Phys.Chem.C 115 Pages: 5271-5279
• [Journal Article] スラブ光導波路分光法を用いた固液界面におけるタンパク質の吸着過程及び機能のその場観察 (2011)
  • Author(s): 松田直樹
  • Journal Title: 色材協会誌 84 Pages: 18-23
• [Journal Article] In situ observation of reduction behavior of cytochrome c adsorbed on glass surface by slab optical waveguide spectroscopy (2011)
  • Author(s): Naoki Matsuda, Hirotaka Okabe, Masaki Fujii, Masayoshi Matsui, Yusuke Ayato, Akiko Takatsu, Kenji Kato
  • Journal Title: IEICE Transactions on Electronics Volume: E94-C Pages: 170-175
  • NAID: 10028231537
  • Peer Reviewed
• [Journal Article] Elucidation of Electrostatic Interaction between Cationic Dyes and Ag Nanoparticles Generating Enrormous SERS Enhancement in Solution (2011)
  • Author(s): Masayuki Futamata, Yingying Yu, Toru Yajima
  • Journal Title: Journal of Physical Chemistry C Volume: 115 Pages: 5271-5279
  • Peer Reviewed
• [Journal Article] スラブ光導波路分光法を用いた固液界面におけるタンパク質の吸着過程及び機能のその場観察 (2011)
  • Author(s): 松田直樹
  • Journal Title: 色材協会誌 Volume: 84 Pages: 18-23
  • Peer Reviewed
• [Journal Article] A Novel Biofuel Cell Based on Direct ElectronTransfer Utilizing Enzymatic Activityof Hemoglobin at Indium-Tin-Oxide Electrodes in Cathodic Process (2009)
  • Author(s): Yusuke Ayato, Naoki Matsuda
  • Journal Title: Chem.Lett. 38 Pages: 504-505
• [Journal Article] Study of adsorption behaviors of meso-tetrakis(4-N-Methylpyridyl)porphinep-Toluenesulfonate at indium-tin-oxide electrode/solution interface by in-situ internal reflection spectroscopy and cyclic voltammetry (2009)
  • Author(s): Shujun Qiua, Lixian Sun, Hailiang Chu, Yongjin Zou, Fen Xu, Naoki Matsuda
  • Journal Title: THIN SOLID FILMS 517 Pages: 2905-2911
  • Peer Reviewed
• [Journal Article] A Novel Biofuel Cell Based on Direct Electron Transfer Utilizing Enzymatic Activity of Hemoglobin at Indium-Tin-Oxide Electrodes in Cathodic Process (2009)
  • Author(s): Yusuke Ayato, Naoki Matsuda
  • Journal Title: Chemistry Letters 38 Pages: 504-505
  • NAID: 10025122384
  • Peer Reviewed
• [Journal Article] LSP spectral changes correlating with SERS activation and quenching for R6G on immobilized Ag nanoparticles (2008)
  • Author(s): Masayuki Futamata, Yoshihiro Maruyama
  • Journal Title: Appl.Phys. B93 Pages: 117-130
• [Journal Article] In Situ Observation of Electrochemical Activity and Time Dependent Characteristics of Cytochrome c at Bare Indium-Tin-Oxide Electrodesby Voltammetry and Slab Optical Waveguide Spectroscopy (2008)
  • Author(s): Yusuke Ayato, Akiko Takatsu, Kenji Kato, Naoki Matsuda
  • Journal Title: IEICE Trans. Electron. E91-C Pages: 1899-1904
• [Journal Article] In Situ Observation of Electrochemical Activity and Time Dependent Characteristics of Cytochrome c at Bare Indium-Tin-Oxide Electrodes by Voltammetry and Slab Optical Waveguide Spectroscopy (2008)
  • Author(s): Yusuke Ayato, Akiko Takatsu, Kenji Kato, Naoki Matsuda
  • Journal Title: IEICE Transaction Electronics E91-C Pages: 1899-1904
  • Peer Reviewed
• [Journal Article] LSP spectral changes correlating with SERS activation and quenching for R6G on immobilized Ag nanoparticles (2008)
  • Author(s): Masayuki Futamata, Yoshihiro Maruyama
  • Journal Title: Applied Physics B93 Pages: 117-130
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): 二又政之
  • Journal Title: 07ICO3/ナノテクノロジー分野 科学技術・研究開発の国際比較 2008年度版(科学技術振興機構(JST))
• [Presentation] Oxidation-reduction reaction of cytochrome c on solid/liquid interfaces : in situ observation by slab optical waveguide spectroscopy (2011)
  • Author(s): Naoki Matsuda, Hirotaka Okabe, Akiko Takatsu, Kenji Kato
  • Organizer: 2010環太平洋国際化学会議
  • Place of Presentation: ハワイコンベンションセンター(ホノルル、アメリカ合衆国)
  • Year and Date: 2011-12-19
• [Presentation] In Situ Observation of Direct Electron Transfer Reaction between Cytochrome c and ITO Electrode by Slab Optical Waveguide Spectroscopy (2011)
  • Author(s): Naoki Matsuda
  • Organizer: 7th International Symposium on Transparent Oxide Thin Films for Electronics and Optics (TOEO-7)
  • Place of Presentation: 国際会議場(早稲田大学)
  • Year and Date: 2011-03-15
• [Presentation] スラブ光導波路分光法を用いた界面におけるタンパク質機能のその場観察 (2011)
  • Author(s): 松田直樹
  • Organizer: 平成22年度新産業支援プラットフォーム事業第三回研究会
  • Place of Presentation: 井門博多駅前ビル
  • Year and Date: 2011-02-23
• [Presentation] Oxidation-reduction reaction of cytochrome c on solid/liquid interfaces : in situ observation by slab optical waveguide spectroscopy (2011)
  • Author(s): Naoki Matsuda
  • Organizer: The Eleventh International Symposium on Biomimetic Materials Processing(BMMP-11)
  • Place of Presentation: 野依記念館(名古屋大学)
  • Year and Date: 2011-01-27
• [Presentation] In Situ Observation of UV-vis.Absorption Spectra of Adsorption Process and Functionalities of Cytochrome c Proteins by Slab Optical Waveguide Spectroscopy (2010)
  • Author(s): Naoki Matsuda, Hirotaka Okabe
  • Organizer: 第20回MRSJ学術シンポジウム
  • Place of Presentation: 横浜情報文化センター
  • Year and Date: 2010-12-22
• [Presentation] Oxidation-reduction reaction of cytochrome c on solid/liquid interfaces : in situ observation by slab optical waveguide spectroscopy (2010)
  • Author(s): Naoki Matsuda, Hirotaka Okabe, Akiko Takatsu, Kenji Kato
  • Organizer: 2010環太平洋国際化学会議
  • Place of Presentation: ハワイコンベンションセンター、ホノルル、アメリカ合衆国(招待講演)
  • Year and Date: 2010-12-19
• [Presentation] In situ observation of protein functionality on solid/liquid interfaces by slab optical waveguide spectroscopy (2010)
  • Author(s): 松田直樹
  • Organizer: The International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 野依記念館、名古屋大学(招待講演)
  • Year and Date: 2010-05-28
• [Presentation] 時間分解スラブ光導波路を用いたITO電極表面におけるチトクロームcの吸着過程と電子移動反応のその場観察 (2010)
  • Author(s): 松田直樹
  • Organizer: 電気化学会第77回大会
  • Place of Presentation: 富山大学
  • Year and Date: 2010-03-30
• [Presentation] In situ observation of protein functionality on solid/liquid interfaces by slab optical waveguide spectroscopy (2010)
  • Author(s): 松田直樹
  • Organizer: BMMP-10
  • Place of Presentation: 野依記念館(名古屋大学)
  • Year and Date: 2010-02-28
• [Presentation] In Situ Observation of Protein Functionalities on Solid/Liquid Interfaces by Slab Optical Waveguide Spectroscopy (2009)
  • Author(s): 松田直樹
  • Organizer: 第19回MRS-Jシンポジウム
  • Place of Presentation: 横浜波止場会館
  • Year and Date: 2009-12-07
• [Presentation] In situ observation of functionality of proteins on solid/liquid interfaces by slab optical waveguide spectroscopy (2009)
  • Author(s): 松田直樹
  • Organizer: The 10th Chitose Internartional Forum
  • Place of Presentation: 千歳科学技術大学
  • Year and Date: 2009-11-13
• [Presentation] スラブ光導波路分光法を用いたITO電極表面の可視吸収スペクトルのその場観察とチトクロームcの電子移動反応 (2009)
  • Author(s): 松田直樹
  • Organizer: 電子情報通信学会2009ソサイエティー大会
  • Place of Presentation: 新潟大学
  • Year and Date: 2009-09-15
• [Presentation] ITO電極に直接吸着したヘムタンパク質の過酸化水素共存下における電子移動反応の観察 (2009)
  • Author(s): 綾戸勇輔、松田直樹
  • Organizer: 電気化学会秋季講演大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2009-09-11
• [Presentation] 分子ネットワークの電気伝導: Cytochrome c/DNA構造体 (2009)
  • Author(s): 瀬川裕司、綾戸勇輔、松田直樹、松本卓也、川合知二
  • Organizer: 応用物理学会第70回秋季講演大会
  • Place of Presentation: 富山大学
  • Year and Date: 2009-09-09
• [Presentation] In situ observation of activity of hemoglobin and cytochrome c on solid/liquid interfaces by slab optical waveguide spectroscopy (2009)
  • Author(s): 松田直樹
  • Organizer: ISAOP-9
  • Place of Presentation: Bunratty Castle Hotel(アイルランド、シャノン)
  • Year and Date: 2009-09-04
• [Presentation] In situ observation of activity of hemoglobin and cytochrome c on solid/liquid interfaces by slab optical waveguide spectroscopy (2009)
  • Author(s): 松田直樹
  • Organizer: 2009 Prague Meetings on Macromolecules
  • Place of Presentation: Institute of Macromolecular Chemistry(プラハ)
  • Year and Date: 2009-07-07
• [Presentation] 表面増強ラマン散乱:アニオンによる電子移動相互作用の活性化 (2009)
  • Author(s): 丸山芳弘, 二又政之
  • Organizer: 2009年春季第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学
  • Year and Date: 2009-03-30
• [Presentation] 固液界面のチップ増強ラマン散乱(TERS) (2009)
  • Author(s): 高橋賢, 二又政之
  • Organizer: 2009年春季第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学
  • Year and Date: 2009-03-30
• [Presentation] SERS化学的増強におけるアニオンの役割 (2009)
  • Author(s): 二又政之, 丸山芳弘
  • Organizer: 第7回プラズモニクス研究会
  • Place of Presentation: 徳島大学
  • Year and Date: 2009-03-06
• [Presentation] SERS化学的増強におけるアニオン効果 (2009)
  • Author(s): 丸山芳弘, 二又政之
  • Organizer: 文科省科研費特定領域「光-分子強結合場」第4回公開シンポジウム
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-01-24
• [Presentation] Comparison of activity of hemoglobin and cytochrome c on solid/liquid interfaces : in situ observation by slab optical waveguide spectroscopy (2009)
  • Author(s): Naoki Matsuda, Yusuke Ayato, Akiko Takatsu, Kenji Kato
  • Organizer: Ninth International Symposium on Biomimetic Materials Processing (BMMP-9)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-01-20
• [Presentation] In situ observation of solid/liquid interfaces by slab optical waveguide spectroscopy (2008)
  • Author(s): Naoki Matauda
  • Organizer: IUMRS-ICA2008
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-12-12
• [Presentation] SERS:化学的増強機構におけるアニオンによる活性化 (2008)
  • Author(s): 丸山芳弘, 二又政之
  • Organizer: 分光学会表面・界面高感度分光部会第1回シンポジウム
  • Place of Presentation: 産総研つくばセンター
  • Year and Date: 2008-12-05
• [Presentation] スラブ光導波路分光法を用いた固液界面における紫外可視吸収スペクトルのその場観察 (2008)
  • Author(s): 松田直樹
  • Organizer: 2008年10月度M&M研究会
  • Place of Presentation: 鳥栖メッセ
  • Year and Date: 2008-10-24
• [Presentation] スラブ光導波路分光法を用いた固液界面における吸収スペクトルのその場測定とセンサーへの応用 (2008)
  • Author(s): 松田直樹
  • Organizer: 第13回POC研究会
  • Place of Presentation: 日本ペイント本社
  • Year and Date: 2008-10-16
• [Presentation] In situ observation of absorption spectra of proteins adsorbed on solid/liquid interfaces by slab optical waveguide spectroscopy (2008)
  • Author(s): 松田直樹
  • Organizer: The 8th International Discussion & Conference on Nano Interface Controlled Electronic Devices : IDC-NICE 2008, POSTECH
  • Place of Presentation: 慶州、大韓民国
  • Year and Date: 2008-10-15
• [Presentation] In situ observation of absorption spectra of proteins adsorbed on solid/liquid interfaces by slab optical waveguide spectroscopy (2008)
  • Author(s): Naoki Matsuda
  • Organizer: The 8th International Discussion & Conference on Nano Interface Controlled Electronic Devices (IDC-NICE 2008)
  • Place of Presentation: POSTECH(大韓民国)
  • Year and Date: 2008-10-15
• [Presentation] Measurement of Potential-current relation with respect to the electrode arrangement in solution plasma (2008)
  • Author(s): Naoki Matsuda, Yusuke Ayato, Tatsuro Nakashima
  • Organizer: 9th Asia-Pacific Conference on Plasma Science and Technology (APCPST) and 21st Symposium on Plasma Science for Materials (SPSM)
  • Place of Presentation: 黄山国際ホテル(中華人民共和国)
  • Year and Date: 2008-10-10
• [Presentation] ITO電極/溶液界面のスラブ光導波路分光法によるその場観察 (2008)
  • Author(s): 松田直樹, 綾戸勇輔
  • Organizer: 電子情報通信学会08年ソサイエティ大会
  • Place of Presentation: 明治大学
  • Year and Date: 2008-09-18
• [Presentation] Anion-induced activation or quenching of SERS signal from R6G on immobilized Ag nanoparticles (2008)
  • Author(s): M. Futamata, Y. Maruyama
  • Organizer: ICORS XXI
  • Place of Presentation: Brunel Univ., London (英国)
  • Year and Date: 2008-08-18
• [Presentation] Fabrication of SERS substrates using EBL or nanosphere overlayer method (2008)
  • Author(s): M. Futamata, D-G. Kim, Y. Maruyama
  • Organizer: ICORS XXI
  • Place of Presentation: Brunel Univ., London (英国)
  • Year and Date: 2008-08-18
• [Presentation] In Situ Observation、Adsorption Process、Functions、Proteins、ITO Electrodes、Electrode/electrolyte Interfaces、Slab Optical Waveguide Spectroscopy (2008)
  • Author(s): 松田直樹
  • Organizer: 第5回国際及び第7回日中合同熱測定シンポジウム : CATS2008
  • Place of Presentation: 中国科学院大連化学物理研究所、大連、中華人民共和国(招待講演)
  • Year and Date: 2008-05-20
• [Presentation] In Situ Observation、Adsorption Process、Functions、Proteins、ITO Electrodes、Electrode/electrolyte Interfaces、Slab Optical Waveguide Spectroscopy (2008)
  • Author(s): Naoki Matsuda
  • Organizer: 第5回国際及び第7回日中合同熱測定シンポジウム(CATS2008)
  • Place of Presentation: 中国科学院大連化学物理研究所(中華人民共和国)
  • Year and Date: 2008-05-20
• [Presentation] スラブ光導波路分光法を用いた固液界面における吸収スペクトルの高感度その場測定 (2008)
  • Author(s): 松田直樹
  • Organizer: フォトニクス・フォーラム
  • Place of Presentation: 豊田中研(招待講演)
  • Year and Date: 2008-05-16
• [Presentation] スラブ光導波路分光法を用いた固液界面における吸収スペクトルの高感度その場測定 (2008)
  • Author(s): 松田直樹
  • Organizer: フォトニクス・フォーラム
  • Place of Presentation: 豊田中研
  • Year and Date: 2008-05-16
• [Presentation] アニオン添加による銀ナノ粒子表面とSERS活性の変化 (2008)
  • Author(s): 丸山芳弘, 二又政之
  • Organizer: ナノ学会第6回大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-05-08
• [Remarks] ホームページ等
  • URL: http://unit.aist.go.jp/msrc/ci/index.html