Development of high-speed and high-efficiency monolithic silica capillary columns for high-performance liquid chromatography

• Project/Area Number: 20350036
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: TANAKA Nobuo Kyoto Institute of Technology, 法人本部, 学長特任補佐 (60127165)
• Co-Investigator(Kenkyū-buntansha): IKEGAMI Tohru 京都工芸繊維大学, 工芸科学研究科, 准教授 (20301252)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: HPLC / モノリスカラム / シリカ / キャピラリー / 高性能化 / 理論段数 / ピークキャパシティ / ナノHPLC / モノリス / プロテオーム解析 / 透過率 / プロテオーム / HILIC / 化学修飾 / キャピラリーカラム / 100万段

Research Abstract

Optimization of preparation conditions of monolithic silica capillary columns attained 10000 and 1000000 theoretical plates with a column dead time of 10s and 5000s, respectively, providing ultrahigh resolution of closely related substances.

Research Products

• [Journal Article] Monolithic silica rod columns for high-efficiency reversed-phase liquid chromatography. (2011)
  • Author(s): S.Miyazaki, M.Takahashi, M.Ohira, H.Terashima, K.Morisato, K.Nakanishi, T.Ikegami, K.Miyabe, N.Tanaka, J.Chromatogr.A
  • Journal Title: 1218 Pages: 1988-1994
  • Peer Reviewed
• [Journal Article] Monolithic silica rod columns for high-efficiency reversed-phase liquid chromatography. (2011)
  • Author(s): S.Miyazaki, 他
  • Journal Title: J.Chromatogr.A Volume: 1218 Pages: 1988-1994
  • Peer Reviewed
• [Journal Article] One-Dimensional Capillary Liquid Chromatographic Separation Coupled with Tandem Mass Spectrometry Unveils the Escherichia coli Proteome on a Microarray Scale. (2010)
  • Author(s): M.Iwasaki, S.Miwa, T.Ikegami, M.Tomita, N.Tanaka, Y.Ishihama, Anal.Chem.
  • Journal Title: 82 Pages: 2616-2620
  • Peer Reviewed
• [Journal Article] Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC. (2010)
  • Author(s): K.Okusa, Y.Suita, Y.Otsuka, M.Tahara, T.Ikegami, N.Tanaka, M.Ohira, M.Takahashi, J.Sep.Sci.
  • Journal Title: 33 Pages: 348-358
  • Peer Reviewed
• [Journal Article] The performance of hybrid monolithic silica capillary columns prepared by changing feed ratios of tetramethoxysilane and methyltrimethoxysilane (2010)
  • Author(s): T.Hara, S.Makino, Y.Watanabe, T.Ikegami, K.Cabrera, B.Smarsly, N.Tanaka, J.Chromatogr.A
  • Journal Title: 1217 Pages: 89-98
  • Peer Reviewed
• [Journal Article] One-dimensional capillary liquid chromatographic separation coupled with tandem mass spectrometry unveils the Escherichia coli proteome on amicroarray scale. (2010)
  • Author(s): M.Iwasaki, 他
  • Journal Title: Anal.Chem. Volume: 82 Pages: 2616-2620
  • Peer Reviewed
• [Journal Article] The performance of hybrid monolithic silica capillary columns prepared by changing feed ratios of tetramethoxysilane and methyltrimethoxysila (2010)
  • Author(s): Takeshi Hara
  • Journal Title: Journal of Chromatography A 1217 Pages: 89-98
  • Peer Reviewed
• [Journal Article] Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC (2010)
  • Author(s): Kensuke Okusa
  • Journal Title: J. Separation Science 33 Pages: 348-358
  • Peer Reviewed
• [Journal Article] One-Dimensional Capillary Liquid Chromatographic Separation Coupled with Tandem Mass Spectrometry Unveils the Escherichia coli Proteome on a Microarray Scale (2010)
  • Author(s): Mio Iwasaki
  • Journal Title: Analytical Chemistry 82 Pages: 2616-2620
  • Peer Reviewed
• [Journal Article] Improvement of separation efficiencies of anion-exchange chromatography using monolithic silica capillary columns modified with polyacrylates and polymethacrylates containing tertiary amino or quaternary ammonium groups. (2009)
  • Author(s): Y.Watanabe, T.Ikegami, K.Horie, T.Hara, J.Jaafar, N.Tanaka, J.Chromatogr.A
  • Journal Title: 1216 Pages: 7394-7401
  • Peer Reviewed
• [Journal Article] Selectivity comparisons of monolithic silica capillary columns modified with poly(octadecyl methacrylate) and octadecyl moieties for halogenated compounds in reversed-phase liquid chromatography. (2009)
  • Author(s): W.Soonthorntantikul, N.Leepipatpiboon, T.Ikegami, N.Tanaka, T.Nhujak, J.Chromatogr.A
  • Journal Title: 1216 Pages: 5868-5874
  • Peer Reviewed
• [Journal Article] Field Enhanced Sample Injection for the CE Determination of Arsenic Compounds Using Successive Multiple Ionic Polymer Layer Coated Capillaries (2009)
  • Author(s): Jafariah Jaafar
  • Journal Title: Chromatographia 69 Pages: 1437-1441
  • Peer Reviewed
• [Journal Article] Selectivity comparisons of monolithic silica capillary columns modified with poly(octadecyl methacrylate) and octadecyl moieties for halogenated compounds in reversed-phase liquid chromatography (2009)
  • Author(s): Wasura Soonthorntantikul
  • Journal Title: Journal of Chromatography A 1216 Pages: 5868-5874
  • Peer Reviewed
• [Journal Article] Improvement of separation efficiencies of anion-exchange chromatography using monolithic silica capillary columns modified with polyacrylates and polymethacrylates containing tertiary amino or quaternary ammonium groups (2009)
  • Author(s): Yuta Watanabe
  • Journal Title: Journal of Chromatography A 1216 Pages: 7394-7401
  • Peer Reviewed
• [Journal Article] Field Enhanced Sample Injection for the Separation and Indirect Detection of Arsenic Compounds by Capillary Electrophoresis Using Successive Multiple Ionic Polymer Layer Coated Capillary (2009)
  • Author(s): Tohru Ikegami, et al.
  • Journal Title: Chromatographia (印刷中, 未定, 掲載决定済)
  • Peer Reviewed
• [Journal Article] Preparation of monolithic silica columns for high-performance liquid chromatography. (2008)
  • Author(s): O.Nunez, K.Nakanishi, N.Tanaka, J.Chromatogr.A
  • Journal Title: 1191 Pages: 231-252
  • Peer Reviewed
• [Journal Article] Separation efficiencies in hydrophilic interaction chromatography (2008)
  • Author(s): T.Ikegami, K.Tomomatsu, H.Takubo, K.Horie, N.Tanaka, J.Chromatogr.A
  • Journal Title: 1184 Pages: 474-503
  • Peer Reviewed
• [Journal Article] High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns. (2008)
  • Author(s): K.Miyamoto, T.Hara, H.Kobayashi, H.Morisaka, D.Tokuda, K.Horie, K.Koduki, S.Makino, O.Nunez, C.Yang, T.Kawabe, T.Ikegami, H.Takubo, Y.Ishihama, N.Tanaka, Anal.Chem.
  • Journal Title: 80 Pages: 8741-8750
  • Peer Reviewed
• [Journal Article] Anion exchange silica monolith for capillary liquid chromatography (2008)
  • Author(s): J.Jaafar , Y.Watanabe, T.Ikegami, K.Miyamoto, N.Tanaka, Anal.Bioanal.Chem
  • Journal Title: 391 Pages: 2551-2556
  • Peer Reviewed
• [Journal Article] Highly efficient analysis of underivatized carbohydrates using monolithic-silica-based capillary hydrophilic interaction (HILIC) HPLC. (2008)
  • Author(s): T.Ikegami, K.Horie, N.Saad, K.Hosoya, O.Fiehn, N.Tanaka, Anal.Bioanal.Chem.
  • Journal Title: 391 Pages: 2533-2542
  • Peer Reviewed
• [Journal Article] Highly Effective Separation and Highly Sensitive Detection of Underivatized Carbohydrates Using a Monolithic Silica Based Capillary Hydrophilic Interacti on (HILIC) HPLC-Electrospray Ionization-Ion Trap Mass Spectrometry. (2008)
  • Author(s): Tohru Ikegami, et al.
  • Journal Title: Anal. Bioanal. Chem. 391 Pages: 2533-2542
  • Peer Reviewed
• [Journal Article] Separation efficiencies in hydrophilic interaction chromatography (総説) (2008)
  • Author(s): T. Ikegami, N. Tanaka, et al.
  • Journal Title: Journal of Chromatography A 1184 Pages: 474-503
  • Peer Reviewed
• [Journal Article] Anion Exchange Silica Monolith for Capillary Liquid Chromatography. (2008)
  • Author(s): Jafariah Jaafar, et al.
  • Journal Title: Anal. Bioanal. Chem. 391 Pages: 2551-2556
  • Peer Reviewed
• [Journal Article] Preparation of monolithic silica columns for high performance liquid chromatography. (総説) (2008)
  • Author(s): Oscar Nunez, et al.
  • Journal Title: Journal of Chromatography A 1191 Pages: 231-252
  • Peer Reviewed
• [Journal Article] High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns (2008)
  • Author(s): Nobuo Tanaka, et al.
  • Journal Title: Anal. Chem. 80 Pages: 8741-8750
  • Peer Reviewed
• [Presentation] High-efficiency monolithic silica columns for HPLC. (2011)
  • Author(s): 田中信男
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy.
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2011-03-15
• [Presentation] High-efficiency monolithic silica columns for HPLC (2011)
  • Author(s): Nobuo Tanaka
  • Organizer: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
  • Place of Presentation: USA, アトランタ国際会議場(招待講演)
  • Year and Date: 2011-03-15
• [Presentation] HILICカラムのテスト法開発 (2010)
  • Author(s): 池上亨
  • Organizer: 第21回クロマトグラフィー科学会議
  • Place of Presentation: 西宮市 武庫川女子大学
  • Year and Date: 2010-10-23
• [Presentation] モノリス型シリカカラムのクロマトグラフィー特性 (2010)
  • Author(s): 田中信男
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: Sendai
  • Year and Date: 2010-09-15
• [Presentation] モノリス型シリカカラムのクロマトグラフィー特性 (2010)
  • Author(s): 田中信男
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 仙台市 東北大学(招待講演)
  • Year and Date: 2010-09-15
• [Presentation] Monolithic silica columns for high speed and high-efficiency HPLC separations (2010)
  • Author(s): N.Tanaka
  • Organizer: 35th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: USA, ボストン国際会議場(招待講演)
  • Year and Date: 2010-06-21
• [Presentation] Monolithic silica columns for high-speed and high-efficiency HPLC Separations. (2010)
  • Author(s): 田中信男
  • Organizer: 35th International Symposium on High Performance Liquid Phase Separations and Related Techniques.
  • Place of Presentation: Boston, USA
  • Year and Date: 2010-06-20
• [Presentation] HPLCカラムの高性能化 (2009)
  • Author(s): 田中信男
  • Organizer: 第20回クロマトグラフィー科学会議
  • Place of Presentation: 東京
  • Year and Date: 2009-11-13
• [Presentation] HPLCカラムの高性能化 (2009)
  • Author(s): 田中信男
  • Organizer: 第20回クロマトグラフィー科学会議
  • Place of Presentation: Tokyo
  • Year and Date: 2009-11-12
• [Presentation] High-efficiency monolithic silica capillary columns for micro-HPLC. (2009)
  • Author(s): 田中信男
  • Organizer: 24th International Symposium on MicroScale Bioseparations.
  • Place of Presentation: Dalian, China.
  • Year and Date: 2009-10-22
• [Presentation] High-efficiency monolithic silica capillary columns for micro-HPLC (2009)
  • Author(s): 田中信男
  • Organizer: 24th International Symposium on MicroScale Bioseparations
  • Place of Presentation: Dalian, China
  • Year and Date: 2009-10-22
• [Presentation] モノリス型シリカキャピラリーカラムを用いる高性能マイクロHPLC (2009)
  • Author(s): 田中信男
  • Organizer: 第225回液体クロマトグラフィー研究懇談会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-13
• [Presentation] High-efficiency monolithic silica columns for HPLC. (2009)
  • Author(s): 田中信男
  • Organizer: 34th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2009-07-01
• [Presentation] High-efficiency monolithic silica columns for HPLC (2009)
  • Author(s): 田中信男
  • Organizer: 34th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2009-07-01
• [Presentation] Preparation and evaluation of monolithic silica columns for hydrophilic interaction (HILIC) chromatography. (2008)
  • Author(s): Tohru Ikegami, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Kyoto
  • Year and Date: 2008-12-04
• [Presentation] Study of peak capacity utilizing monolithic silica capillary columns in liquid chromatography - mass spectrometry. (2008)
  • Author(s): Hirotaka Takubo, Tohru Ikegami, Nobuo Tanaka, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Kyoto
  • Year and Date: 2008-12-03
• [Presentation] Development of high-efficiency ion-exchange monolithic silica columns. (2008)
  • Author(s): Yuta Watanabe, Tohru Ikegami, Nobuo Tanaka, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques.
  • Place of Presentation: Kyoto
  • Year and Date: 2008-12-03
• [Presentation] Preparation of monolithic silica capillary columns for chiral separation by on-column polymerization, and the evaluation of their separation efficiency. (2008)
  • Author(s): Tohru Ikegami, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques.
  • Place of Presentation: Kyoto
  • Year and Date: 2008-12-03
• [Presentation] Selectivity comparison of monolithic silica capillary columns modified with poly(octadecyl methacrylate) and octadecyl moiety for halogenated compounds in reversed-phase HPLC. (2008)
  • Author(s): Thumnoon NHUJAK, Tohru Ikegami, Nobuo Tanaka, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques.
  • Place of Presentation: Kyoto
  • Year and Date: 2008-12-03
• [Presentation] Two-dimensional capillary HPLC in a reversed-phase mode using two C18 monolithic silica columns modified with octadecyl moiety and poly(octadecyl methacrylate). (2008)
  • Author(s): Thumnoon NHUJAK, Tohru Ikegami, Nobuo Tanaka, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques.
  • Place of Presentation: Kyoto
  • Year and Date: 2008-12-03
• [Presentation] High efficiency monolithic silica capillary columns for micro-HPLC. (2008)
  • Author(s): 田中信男, 他
  • Organizer: Asia-Pacific International Symposium on Microscale Separations and Analysis
  • Place of Presentation: Kaoshung, ROC Taiwan.
  • Year and Date: 2008-11-03
• [Presentation] Two-dimensional capillary HPLC in a reversed-phase mode using two monolithic silica C18 columns modified with octadecylsilyl- (N,N- diethylamino)silane and poly(octadecyl methacrylate. (2008)
  • Author(s): Thumnoon NHUJAK, Tohru Ikegami, Nobuo Tanaka, 他.
  • Organizer: International Symposium on Chromatography
  • Place of Presentation: Munster, Germany.
  • Year and Date: 2008-09-22
• [Presentation] High performance monolithic silica columns for reversed-phase, HILIC, and ion-exchange mode HPLC. (2008)
  • Author(s): 田中信男, 他
  • Organizer: 19th International Symposium on Pharmaceutical and Biomedical Analysis (PBA2008)
  • Place of Presentation: Gdansk, Poland.
  • Year and Date: 2008-06-09
• [Presentation] High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns. (2008)
  • Author(s): 田中信男, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques.
  • Place of Presentation: Baltimore, USA.
  • Year and Date: 2008-05-12
• [Presentation] High-efficiency liquid chromatographic separation utilizing long monolithic silica capillary columns (2008)
  • Author(s): Nobuo Tanaka, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Baltimore, USA
• [Presentation] Two dimensional capillary HPLC in a reversed-phase mode using two monolithic silica C18 columns modified with octadecylsilyl-(N, N-diethylamino) silane and poly (octadecyl methacrylate) (2008)
  • Author(s): Thumnoon NHUJAK, 他
  • Organizer: International Symposium on Chromatography
  • Place of Presentation: Munster, Germany
• [Presentation] High efficiency monolithic silica capillary columns for micro-HPLC (2008)
  • Author(s): Nobuo Tanaka, 他
  • Organizer: Asia-Pacific International Symposium on Microscale Separations and Analysis
  • Place of Presentation: Kaoshung, ROC Taiwan
• [Presentation] Two-dimensional capillary HPLC in a reversed-phase mode using two C18 monolithic silica columns modified with octadecyl moiety and poly (octadecyl methacrylate) (2008)
  • Author(s): Thumnoon NHUJAK, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 京都
• [Presentation] Selectivity comparison of monolithic silica capillary columns modified with poly (octadecyl methacrylate) and octadecyl moiety for halogenated compounds in reversed-phase HPLC (2008)
  • Author(s): Thumnoon NHUJAK, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 京都
• [Presentation] Preparation and evaluation of monolithic silica columns for hydrophilic interaction (HILIC) chromatography (2008)
  • Author(s): Tohru Ikegami, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 京都
• [Presentation] Preparation of monolithic silica capillary columns for chiral separation by on-column polymerization, and the evaluation of their separation efficiency (2008)
  • Author(s): Tohru Ikegami, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 京都
• [Presentation] Development of high-efficiency ion-exchange monolithic silica columns (2008)
  • Author(s): Yuta Watanabe, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 京都
• [Presentation] Study of peak capacity utilizing monolithic silica capillary columns in liquid chromatography - mass spectrometry (2008)
  • Author(s): Hirotaka Takubo, 他
  • Organizer: International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 京都
• [Book] Monolithic Silicas in Separation Science(The basic idea and the drivers.) (2011)
  • Author(s): N.Tanaka, K.K.Unger, Ed.K.K.Unger, N.Tanaka, E.Machtejevas
  • Publisher: Wiley
• [Book] Monolithic Silicas in Separation Science(Preparation and properties of various types of monolithic silica stationary phases for reversed-phase, hydrophilic interaction, and ion-exchange chromatography based on polymer-coated materials.) (2011)
  • Author(s): O.Nunez, T.Ikegami, Ed.K.K.Unger, N.Tanaka, E.Machtejevas
  • Publisher: Wiley
• [Book] In "Monolithic Silicas in Separation Science"(High-speed and high-efficiency separations by utilizing monolithic silica capillary columns.)(E.Machtejeves, N.Tanaka, K.K.Unger, ed.) (2011)
  • Author(s): T.Hara, K.Miyamoto, S.Makino, S.Miwa, M.Ohira, N.Tanaka
  • Publisher: Wiley
• [Book] Monolithic Silicas in Separation Science (2011)
  • Author(s): K.K.Unger, N.Tanaka, E.Machtejevas編
  • Total Pages: 344
  • Publisher: Wiley-VCH
• [Book] The basic idea and the drivers. (2011)
  • Author(s): N.Tanaka, K.K.Unger
  • Publisher: In "Monolithic Silicas in Separation Science", K.K.Unger, N.Taka, E.Machtejevas ed.Wiley-VCH
• [Book] Preparation and properties of various types of monolithic silica stationary phases for reversed-phase, hydrophilic interaction, and ion-exchange chromatography based on polymer-coated materials. (2011)
  • Author(s): O.Nunez, T.Ikegami
  • Publisher: In "Monolithic Silicas in Separation Science", K.K.Unger, N.Tanaka, E.Machtejevas ed.Wiley-VCH
• [Book] High-speed and high-efficiency separations by utilizing monolithic silica capillary columns. (2011)
  • Author(s): T.Hara, 他
  • Publisher: In "Monolithic Silicas in Separation Science", K.K.Unger, N.Tanaka, E.Machtejevas ed.Wiley-VCH
• [Book] High-efficiency liquid chromatography separations achieved by monolithic silica columns. (2011)
  • Author(s): K.Miyamoto, 他
  • Publisher: In "Extreme Chromatography : Faster, Hotter, Smaller" W.C.Byrdwell, M.Holcapek, ed.AOCS Press.(印刷中)
• [Book] Monolithic silica capillary columns (2010)
  • Author(s): N.Tanaka
  • Publisher: Wiley(印刷中)
• [Book] Frontiers in Chemistry((In Japanese) High-speed and high-efficiency HPLC separations by using monolithic silica columns.)(T.Kitamori, Ed.) (2008)
  • Author(s): N.Tanaka
  • Publisher: Kagaku Dojin.
• [Book] Multidimensional Liquid Chromatography : Theory and Applications in Industrial Chemistry and the Life Sciences(Monolithic columns and their 2D-HPLC applications.)(S.Cohen and M.Schure Ed.)ISBN:978-0-471-73847-3 (2008)
  • Author(s): T.Ikegami, H.Aoki, H.Kimura, K.Hosoya, N.Tanaka
  • Publisher: John and Wiley and Sons.
• [Book] Multidimensional Liquid Chromatography : Theory and Applications in Industrial Chemistry and the Life Sciences(Monolithic columns and their 2D-HPLC applications.) (2008)
  • Author(s): T.Ikegami, N.Tanaka, et al.
  • Publisher: John and Wiley and Sons.
• [Book] Monolithic columns and their 2D-HPLC applications. In "Multidimensional Liquid Chromatography : Theory and Applications in Industrial Chemistry and the Life Sciences" (2008)
  • Author(s): T. Ikegami, N. Tanaka, et al
  • Publisher: John and Wiley and Sons
• [Book] In "Extreme Chromatography : Faster, Hotter, Smaller" (High-efficiency liquid chromatography separations achieved by monolithic silica columns.)(W.C.Byrdwell and M.Holcapek, ed.)
  • Author(s): K.Miyamoto, T.Ikegami, T.Hara, Y.Ishihama, N.Tanaka
  • Publisher: AOCS Press.In press.
• [Remarks] ホームページ等
  • URL: http://repository.lib.kit.ac.jp/dspace/handle/10212/1960
• [Remarks] 田中信男HPLC分離カラム開発と精密分離