Exciton-controlled imaging for effective spatiotemporal analysis of nucleic acid functions
| Project/Area Number |
20350083
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
OKAMOTO Akimitsu The Institute of Physical and Chemical Research, 岡本独立主幹研究ユニット, ユニットリーダー(独立主幹研究員) (60314233)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
|
|---|
| Keywords | 励起子相互作用 / 蛍光イメージング / 蛍光色素 / DNA / 核酸 / 蛍光 / 色素 / イメージング |
|---|
| Research Abstract |
A series of near-infrared fluorescent probes was designed based on the concept of emission control caused by interdye excitonic interaction. The fluorescent probes showed very weak emission in the unhybridized state, whereas they emitted near-infrared fluorescence after hybridization with the complementary nucleic acid. The hybridization-dependent switching of fluorescence emission made it possible to monitor mRNA in a human cell in the range of near-infrared wavelengths.
|
Report
(4 results)
Research Products
(60 results)
