Densign and Development of Stress Responsive LIPOzyme Reactor Based on Re-functionalization of Junk Peptides
| Project/Area Number |
20360350
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Osaka University |
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Principal Investigator |
UMAKOSHI Hiroshi Osaka University, 大学院・基礎工学研究科, 准教授 (20311772)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOI Ryoichi 大阪大学, 名誉教授 (40029567)
SHIMANOUCHI Toshinori 大阪大学, 大学院・基礎工学研究科, 助教 (10335383)
SHIOMORI Koichiro 宮崎大学, 工学研究科, 准教授 (80235506)
NAKAMURA Hidemi 奈良工業高等専門学校, 物質化学工学科, 教授 (70198232)
MORITA Seiichi 和歌山工業高等専門学校, 物質工学科, 准教授 (70332054)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | メンブレン・ストレスバイオテクノロジー / LIPOzyme / 生体膜 / バイオリアクター / 人工酵素 / ペプチド / リフォールディング / ストレス応答 / LIPOzvme |
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| Research Abstract |
It is demonstrated that the function of LIPOzyme (Liposome+Enzyme) could be induced by the construction of catalytic sites on the liposome membrane as a model biomembrane. The LIPOzyme function could be classified by three categories : (i) electron transfer ; (ii) proton transfer ; (iii) nucleophilic/electrophilic reaction. The LIPOzyme function could be, herewith, controlled by the tuning of junk peptides, ligands or their metal complexes to achieve the desired function. Furthermore, the immobilization of LIPOzyme into the module was presented to develop the novel membrane module. This technique is a promising method to develop the reactor/separator based on the LIPOzyme function. Besides, the mapping of the physicochemical properties of junk peptides and the lipid composition could be performed to clarify the optimal condition for the desired functions of LIPOzyme, resulting in developing their database.
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Report
(4 results)
Research Products
(97 results)
