Structural regulation of amyloid forming proteins with pyrroloquinoline quinone

• Project/Area Number: 20360369
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: SODE Koji Tokyo University of Agriculture and Technology, 大学院・工学研究院, 教授 (10187883)
• Co-Investigator(Kenkyū-buntansha): IKEBUKURO Kazunori 東京農工大学, 大学院・工学研究院, 教授 (70251494) ; TSUGAWA Wakako 東京農工大学, 大学院・工学研究院, 准教授 (80376871) ; FERRI Stefano 東京農工大学, 大学院・工学研究院, 助教 (90334474)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 生物機能工学 / アミロイド / αシヌクレイン / Aβ / プリオン / 構造制御 / 蛋白質 / バイオテクノロジ / 生体分子 / PQQ / 細胞毒性 / 膜感受性蛍光色素 / バイオテクノロジー / ペプチド / 凝集線維化 / 神経変性疾患

Research Abstract

The development of small molecules inhibiting amyloid formation of various proteins, such as alpha synuclein, the causative protein of Parkinson's disease, were investigated. The partial peptides modified with pyrroloquinoline quinone (PQQ) were found to specifically inhibit the amyloid formation of parental protein, but not other amyloid forming proteins.

Research Products

• [Journal Article] Pyrroloquinoline Quinone Inhibits the Fibrillation of Amyloid Proteins (2010)
  • Author(s): J.Kim, M.Kobayashi, M.Fukuda, D.Ogasawara, N.Kobayashi, S.Han, C.Nakamura, M.Inada, C.Miyaura, K.Ikebukuro, K.Sode
  • Journal Title: Prion 4(1) Pages: 26-31
  • Peer Reviewed
• [Journal Article] The inhibitory effect of pyrroloquinoline quinine on the amyloid formation and cytotoxicity of truncated alpha-synuclein (2010)
  • Author(s): J.Kim, R.Harada, M.Kobayashi, N.Kobayahi, K.Sode
  • Journal Title: Molecular Neurodegeneration 20
  • Peer Reviewed
• [Journal Article] The inhibitory effect of pyrroloquinoline quinine on the amyloid formation and cytotoxicity of truncated alpha-synuclein (2010)
  • Author(s): J.Kim, R.Harada, M.Kobayashi, N.Kobayahi, K.Sode
  • Journal Title: Molecular Neurodegeneration Volume: 20 Pages: 20-20
  • Peer Reviewed
• [Journal Article] Pyrroloquinoline Quinone Inhibits the Fibrillation of Amyloid Proteins (2010)
  • Author(s): Jihoon Kim, Masaki Kobayashi, Makoto Fukuda, Daisuke Ogasawara, Natsuki Kobayashi, Sungwoong Han, Chikashi Nakamura, Masaki Inada, Chisato Miyaura, Kazunori Ikebukuro, Koji Sode
  • Journal Title: Prion 2010 Jan.4^<th> 4(1)(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] The inhibition of amyloid fibrillation using the proteolytic products of PQQ-modified α-synuclein (2009)
  • Author(s): N.Kobayashi, J.Kim, K.Ikebukuro, K.Sode
  • Journal Title: The Open Biotechnology J. 3 Pages: 40-45
  • Peer Reviewed
• [Journal Article] The Effect of Amino Acid Substitution in the Imperfect Repeat Sequences of α-Synuclein on Fibrillation (2009)
  • Author(s): R.Harada, N.Kobayashi, J.Kim, C.Nakamura, S.Han, K.Ikebukuro, K.Sode
  • Journal Title: Biochim Biophys Acta. 1792(10) Pages: 998-1003
  • Peer Reviewed
• [Journal Article] The Effect of Amino Acid Substitution in the Imperfect Repeat Sequences of α-Synuclein on Fibrillation (2009)
  • Author(s): Ryuichi Harada, Natsuki Kobayashi, Jihoon Kim, Chikashi Nakamura, Sungwoong Han, Kazunori Ikebukuro, Koji Sode
  • Journal Title: Biochim Biophys Acta. 1792(10) Pages: 998-1003
  • Peer Reviewed
• [Journal Article] The inhibition of amyloid fibrillation using the proteolytic products of PQQ-modified α-synuclein (2009)
  • Author(s): Natsuki, Kobayashi, Jihoon Kim, Kazunori, Ikebukuro, Koji Sode
  • Journal Title: The Open Biotechnology Journal (In press)
  • Peer Reviewed
• [Presentation] アミロイド形成蛋白質細胞毒性用蛍光バイオセンシングの開発 (2010)
  • Author(s): 金志勲, 戸田礼, 佐々木泰彦, 早出広司
  • Organizer: 電気化学会
  • Place of Presentation: 神奈川工科大学、厚木
  • Year and Date: 2010-09-02
• [Presentation] アミロイド形成蛋白質細胞毒性用蛍光バイオセンシングの開発 (2010)
  • Author(s): 金志勲, 戸田礼, 佐々木泰彦, 早出広司
  • Organizer: 電気化学会
  • Place of Presentation: 神奈川工科大学(厚木)
  • Year and Date: 2010-09-02
• [Presentation] The development of a novel theranostic platform for cytotoxicity evaluation of amyloid-forming neurodegenerative disease causative proteins (2010)
  • Author(s): J Kim, R Harada, N Kobayashi, K Ikebukuro, K Sode
  • Organizer: Biosensor 2010
  • Place of Presentation: Glasgo, UK
  • Year and Date: 2010-05-26
• [Presentation] The development of a novel theranostic platform for cytotoxicity evaluation of a myloid-forming neurodegenerative disease causative proteins (2010)
  • Author(s): J Kim, R Harada, N Kobayashi, KIkebukuro,K Sode
  • Organizer: Biosensor 2010
  • Place of Presentation: イギリス グラスゴー
  • Year and Date: 2010-05-26
• [Presentation] アミロイド蛋白質細胞毒性バイオセンシングのセラノスティクスへの応用 (2010)
  • Author(s): 金志勲、小林夏季、池袋一典、早出広司
  • Organizer: 日本化学会(CSJ Student Presentation Award 2010)
  • Place of Presentation: 近畿大学
• [Presentation] The prevention of α-synuclein amyloid formation using PQQ-modified partial peptide fragments : for target specific prevention of the amyloid formation (2009)
  • Author(s): N.Kobayashi, K.Sode
  • Organizer: 9th International Conference AD/PD 2009
  • Place of Presentation: Prague (Czech Republic)(Best Poster Award)
  • Year and Date: 2009-03-15
• [Presentation] The prevention of α-synuclein amyloid formation using PQQ-modified partial peptide fragments: for target specific prevention of the amyloid formation (2009)
  • Author(s): Natsuki Kobayashi
  • Organizer: 9th International Conference AD/PD
  • Place of Presentation: Prague (Czech Republic)
  • Year and Date: 2009-03-15
• [Presentation] 膜蛋白質感受性色素を用いたアミロイド形成蛋白質の新規細胞毒性評価用バイオセンシングシステムの開発 (2009)
  • Author(s): 金志勲、小林夏季、池袋一典、早出広司
  • Organizer: 蛋白質科学会
  • Place of Presentation: 熊本(ポスター賞)
• [Presentation] 蛍光偏向解消法を用いたαシヌクレインオリゴマーの検出 (2009)
  • Author(s): 原田龍一、小林夏季、金志勲、池袋一典、早出広司
  • Organizer: 蛋白質科学会
  • Place of Presentation: 熊本
• [Presentation] 切断型αシヌクレインの凝集・線維化におけるピロロキノリンキノン(PQQ)の影響 (2009)
  • Author(s): Kim Jihoon、小林雅樹、小林夏季、早出広司
  • Organizer: 蛋白質科学会
  • Place of Presentation: 熊本全日空ホテル(熊本県)
• [Presentation] 蛍光偏向解消法を用いたαシヌクレインオリゴマーの検出 (2009)
  • Author(s): 原田龍一、小林夏季、金志勲、池袋一典、早出広司
  • Organizer: 蛋白質科学会
  • Place of Presentation: 熊本全日空ホテル(熊本県)
• [Book] Nanomedicine and the nervous system.(Biomolecular engineering for the regulation of alpha-synuclein nanostructure～toward the development of alpha-synuclein targeting nanomedicine～)
  • Author(s): N.Kobayashi, J.Kim, K.Sode
  • Publisher: Science Publishers(in press)
• [Book] Nanomedicince and the nervous system.以下の章を分担Biomolecular engineering for the regulation of alpha-synuclein nanostructure～toward the development of alpha-synuclein targeting nanomedicine～
  • Author(s): Natsuki Kobayashi, Jihoon Kim, Koii Sode
  • Publisher: Science Publishers